Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   M2I94_RS25315 Genome accession   NZ_CP096950
Coordinates   5385801..5387021 (+) Length   406 a.a.
NCBI ID   WP_283859360.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY5532     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5380801..5392021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2I94_RS25285 (M2I94_25200) fliR 5380901..5381678 (+) 778 Protein_4999 flagellar biosynthetic protein FliR -
  M2I94_RS25290 (M2I94_25205) - 5381681..5381941 (+) 261 Protein_5000 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  M2I94_RS25295 (M2I94_25210) - 5382938..5383180 (+) 243 WP_023082027.1 hypothetical protein -
  M2I94_RS25305 (M2I94_25220) pilA 5383435..5383887 (-) 453 WP_114064701.1 pilin Machinery gene
  M2I94_RS25310 (M2I94_25225) pilB 5384119..5385797 (+) 1679 Protein_5003 type IV-A pilus assembly ATPase PilB -
  M2I94_RS25315 (M2I94_25230) pilC 5385801..5387021 (+) 1221 WP_283859360.1 type 4a pilus biogenesis protein PilC Machinery gene
  M2I94_RS25320 (M2I94_25235) pilD 5387025..5387897 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  M2I94_RS25325 (M2I94_25240) coaE 5387894..5388505 (+) 612 WP_010793792.1 dephospho-CoA kinase -
  M2I94_RS25330 (M2I94_25245) yacG 5388502..5388702 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  M2I94_RS25335 (M2I94_25250) - 5388739..5388948 (-) 210 WP_003094660.1 hypothetical protein -
  M2I94_RS25340 (M2I94_25255) - 5389054..5389743 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  M2I94_RS25345 (M2I94_25260) - 5389740..5390210 (-) 471 WP_010793791.1 hypothetical protein -
  M2I94_RS25350 (M2I94_25265) - 5390207..5390632 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  M2I94_RS25355 (M2I94_25270) - 5390766..5391395 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  M2I94_RS25360 (M2I94_25275) - 5391392..5391841 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44302.06 Da        Isoelectric Point: 9.8784

>NTDB_id=685571 M2I94_RS25315 WP_283859360.1 5385801..5387021(+) (pilC) [Pseudomonas aeruginosa strain NY5532]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNTLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGAIGFTFK
ELHKRSKKFRDALDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=685571 M2I94_RS25315 WP_283859360.1 5385801..5387021(+) (pilC) [Pseudomonas aeruginosa strain NY5532]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTCAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGTTGGTGGATGA
GATCAAGCAGGAAGTGTCCTCGGGCAACACCCTGGCCAACTCACTGAGAAAGAAACCACAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACCTACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTTCTGATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAGTTCCTACAGGAGTGGTGGCTTGCCGTCATCGTTGGTGTGGGGGCGATTGGCTTTACCTTCAAG
GAACTGCATAAACGTTCGAAAAAATTCCGCGACGCACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCCGCGGTCGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCAGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

76.79

99.754

0.766
  pilC Acinetobacter baumannii D1279779

61.139

99.507

0.608
  pilC Acinetobacter baylyi ADP1

60.837

100

0.608
  pilC Legionella pneumophila strain ERS1305867

54.545

97.537

0.532
  pilG Neisseria meningitidis 44/76-A

45.409

99.261

0.451
  pilG Neisseria gonorrhoeae MS11

45.161

99.261

0.448
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

41.562

97.783

0.406
  pilC Thermus thermophilus HB27

37.157

98.768

0.367