NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	M2I94_RS25305	Genome accession	NZ_CP096950
Coordinates	5383435..5383887 (-)	Length	150 a.a.
NCBI ID	WP_114064701.1	Uniprot ID	-
Organism	Pseudomonas aeruginosa strain NY5532
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5378435..5388887

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2I94_RS25270 (M2I94_25185)	-	5378890..5379552 (+)	663	WP_033992423.1	DUF3218 family protein	-
M2I94_RS25275 (M2I94_25190)	fliP	5379835..5380587 (+)	753	WP_023435066.1	flagellar type III secretion system pore protein FliP	-
M2I94_RS25280 (M2I94_25195)	fliQ	5380632..5380901 (+)	270	WP_003083053.1	flagellar biosynthesis protein FliQ	-
M2I94_RS25285 (M2I94_25200)	fliR	5380901..5381678 (+)	778	Protein_4999	flagellar biosynthetic protein FliR	-
M2I94_RS25290 (M2I94_25205)	-	5381681..5381941 (+)	261	Protein_5000	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
M2I94_RS25295 (M2I94_25210)	-	5382938..5383180 (+)	243	WP_023082027.1	hypothetical protein	-
M2I94_RS25305 (M2I94_25220)	pilA	5383435..5383887 (-)	453	WP_114064701.1	pilin	Machinery gene
M2I94_RS25310 (M2I94_25225)	pilB	5384119..5385797 (+)	1679	Protein_5003	type IV-A pilus assembly ATPase PilB	-
M2I94_RS25315 (M2I94_25230)	pilC	5385801..5387021 (+)	1221	WP_283859360.1	type 4a pilus biogenesis protein PilC	Machinery gene
M2I94_RS25320 (M2I94_25235)	pilD	5387025..5387897 (+)	873	WP_003125204.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
M2I94_RS25325 (M2I94_25240)	coaE	5387894..5388505 (+)	612	WP_010793792.1	dephospho-CoA kinase	-
M2I94_RS25330 (M2I94_25245)	yacG	5388502..5388702 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 150 a.a. Molecular weight: 15471.67 Da Isoelectric Point: 7.7827

                                >NTDB_id=685570 M2I94_RS25305 WP_114064701.1 5383435..5383887(-) (pilA) [Pseudomonas aeruginosa strain NY5532]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGENIKIGSQASTEDTTYV

GVDATANKLGTIAVTIGNNGAGTVTFTFAAGNSSPKNSGKIITLTRTDAGSWACTSTQDEMFIPKGCKAP

Nucleotide

Download Length: 453 bp

                                >NTDB_id=685570 M2I94_RS25305 WP_114064701.1 5383435..5383887(-) (pilA) [Pseudomonas aeruginosa strain NY5532]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT

TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCACTGAAGACCACTGTTG

AAGAGTCGCTGTCGCGTGGAATCGCTGGGGAAAATATTAAAATTGGTTCCCAAGCATCTACTGAAGATACTACCTATGTA

GGTGTTGATGCTACCGCAAATAAGCTTGGCACTATTGCTGTGACAATTGGGAACAATGGCGCAGGTACCGTGACTTTCAC

TTTTGCAGCCGGCAATTCCAGTCCGAAAAACTCCGGTAAAATTATCACCCTGACCCGGACTGATGCAGGGTCGTGGGCTT

GTACTTCTACCCAGGACGAAATGTTCATCCCTAAAGGTTGCAAAGCGCCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	68.456	99.333	0.68
pilA/pilAI	Pseudomonas stutzeri DSM 10701	47.333	100	0.473
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	43.671	100	0.46
pilA	Vibrio cholerae strain A1552	43.671	100	0.46
pilA	Vibrio cholerae C6706	43.671	100	0.46
pilA	Vibrio parahaemolyticus RIMD 2210633	46.259	98	0.453
pilA	Acinetobacter baumannii strain A118	44.295	99.333	0.44
pilA2	Legionella pneumophila strain ERS1305867	42.282	99.333	0.42
comP	Acinetobacter baylyi ADP1	40.909	100	0.42
pilA/pilAII	Pseudomonas stutzeri DSM 10701	43.357	95.333	0.413
pilA2	Legionella pneumophila str. Paris	41.611	99.333	0.413

Detailed information NTG0685570