Detailed information NTG0684356

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M1Q10_RS08415
• Genome accession: NZ_CP096813
• Coordinates: 1813391..1814539 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 8D
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1808391..1819539

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M1Q10_RS08390 (M1Q10_08390)	-	1809542..1809838 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
M1Q10_RS08395 (M1Q10_08395)	-	1809835..1810428 (-)	594	WP_003084544.1	YggT family protein	-
M1Q10_RS08400 (M1Q10_08400)	proC	1810439..1811260 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
M1Q10_RS08405 (M1Q10_08405)	-	1811272..1811964 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
M1Q10_RS08410 (M1Q10_08410)	pilT	1812179..1813213 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M1Q10_RS08415 (M1Q10_08415)	pilU	1813391..1814539 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
M1Q10_RS08420 (M1Q10_08420)	czcD	1814547..1815446 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
M1Q10_RS08425 (M1Q10_08425)	-	1815600..1816004 (+)	405	WP_003084560.1	NINE protein	-
M1Q10_RS08430 (M1Q10_08430)	-	1816247..1817620 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
M1Q10_RS08435 (M1Q10_08435)	-	1817617..1818801 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=684356 M1Q10_RS08415 WP_003100959.1 1813391..1814539(+) (pilU) [Pseudomonas aeruginosa strain 8D]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=684356 M1Q10_RS08415 WP_003100959.1 1813391..1814539(+) (pilU) [Pseudomonas aeruginosa strain 8D]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382