Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PALA39_RS27450 Genome accession   NZ_CP109920
Coordinates   5743012..5743476 (-) Length   154 a.a.
NCBI ID   WP_023090842.1    Uniprot ID   A3RJ48
Organism   Pseudomonas aeruginosa strain PALA39     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5738012..5748476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA39_RS27435 (PALA39_05422) nadC 5740512..5741360 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PALA39_RS27445 (PALA39_05424) - 5741541..5742854 (-) 1314 WP_023090841.1 O-antigen ligase family protein -
  PALA39_RS27450 (PALA39_05425) pilA 5743012..5743476 (-) 465 WP_023090842.1 pilin Machinery gene
  PALA39_RS27455 (PALA39_05426) pilB 5743707..5745407 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA39_RS27460 (PALA39_05427) pilC 5745411..5746628 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  PALA39_RS27465 (PALA39_05428) pilD 5746629..5747501 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA39_RS27470 (PALA39_05429) coaE 5747498..5748109 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  PALA39_RS27475 (PALA39_05430) yacG 5748106..5748306 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16023.30 Da        Isoelectric Point: 9.0066

>NTDB_id=681632 PALA39_RS27450 WP_023090842.1 5743012..5743476(-) (pilA) [Pseudomonas aeruginosa strain PALA39]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSSDTPGNNEYDLGFTS
STLLTGSGKGQIKIDKADTATPEISGTLGNSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKTNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=681632 PALA39_RS27450 WP_023090842.1 5743012..5743476(-) (pilA) [Pseudomonas aeruginosa strain PALA39]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAGCGATACCCCCGGAAACAATGAATATGATCTTGGCTTTACCAGC
TCTACTCTGCTTACTGGTAGCGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
TCTGGGCAACTCTTCTGGTAAGGGTATCGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAACCAACTACGCCCCGGCTAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3RJ48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

43.949

100

0.448
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.516

100

0.448
  pilA Pseudomonas aeruginosa PAK

41.139

100

0.422
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.447

98.701

0.409
  pilA Vibrio cholerae strain A1552

41.447

98.701

0.409
  pilA Vibrio cholerae C6706

41.447

98.701

0.409
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.94

96.753

0.396
  pilA Ralstonia pseudosolanacearum GMI1000

35.882

100

0.396