Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MTO69_RS01045 Genome accession   NZ_CP096199
Coordinates   222827..224146 (+) Length   439 a.a.
NCBI ID   WP_248330364.1    Uniprot ID   -
Organism   Vibrio sinaloensis strain YA2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 217827..229146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTO69_RS01025 (MTO69_01025) pilM 220442..221386 (+) 945 WP_248330356.1 pilus assembly protein PilM -
  MTO69_RS01030 (MTO69_01030) - 221374..221925 (+) 552 WP_248330358.1 PilN domain-containing protein -
  MTO69_RS01035 (MTO69_01035) pilO 221915..222445 (+) 531 WP_248330360.1 type 4a pilus biogenesis protein PilO -
  MTO69_RS01040 (MTO69_01040) - 222432..222830 (+) 399 WP_248330362.1 hypothetical protein -
  MTO69_RS01045 (MTO69_01045) pilQ 222827..224146 (+) 1320 WP_248330364.1 type IV pilus secretin PilQ Machinery gene
  MTO69_RS01050 (MTO69_01050) aroK 224305..224823 (+) 519 WP_248330366.1 shikimate kinase AroK -
  MTO69_RS01055 (MTO69_01055) aroB 224849..225937 (+) 1089 WP_248330368.1 3-dehydroquinate synthase -
  MTO69_RS01060 (MTO69_01060) - 226025..227512 (+) 1488 WP_248330370.1 AAA family ATPase -
  MTO69_RS01065 (MTO69_01065) - 227610..228437 (+) 828 WP_248330372.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 47369.17 Da        Isoelectric Point: 5.1082

>NTDB_id=680242 MTO69_RS01045 WP_248330364.1 222827..224146(+) (pilQ) [Vibrio sinaloensis strain YA2]
MSDWRKMAQVITTCSYLLLFNWMLLSTCFATEQGDSASLSHHRLSFNFQDISVRQALRIIADSQNINIAIAHSVEGQLTL
RLNQVTGQQALDTIVEMQGLQQQVKQGVVMISPKVPSAALGRDLTTSMIALQYADASELAQVLQGEQGVGLLSERGAVSV
DKRTNVILVRDLADNIAAAREMVAMLDVPVKQVQIEARIVTINQGNLEALGVRWGISHSGRHGQVGGTIEDLNLDSASID
QQLNVNLAAQAVNAASIAFQIAKLGSDTLLDLELSALQRESKAEVISSPSLMTTHKQAAFIEQGTEIPYLESSSSGATNV
AFKKAVLSLKVTPQITPNNQVILDLNVTQDRPGDVVNTGTGEAVAIDTQRIGTQVLVESGQTIVLGGIFQHSVLESVDKV
PLLGDIPLLGGLFRRTYQKIGKRELVVFVTPKVITEFSE

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=680242 MTO69_RS01045 WP_248330364.1 222827..224146(+) (pilQ) [Vibrio sinaloensis strain YA2]
ATGAGTGACTGGCGCAAAATGGCGCAAGTCATCACGACTTGTTCCTACCTACTTCTCTTCAATTGGATGTTGCTTTCTAC
TTGCTTCGCCACAGAGCAAGGCGACAGCGCGTCACTCTCTCACCATAGGTTGTCATTTAACTTCCAAGATATCTCTGTGC
GCCAAGCGCTGCGAATTATTGCCGATAGTCAAAATATCAATATTGCCATTGCTCACTCCGTTGAGGGCCAATTGACGTTG
CGTTTGAACCAAGTCACAGGGCAACAAGCACTTGATACCATTGTTGAGATGCAAGGCTTGCAGCAGCAGGTTAAGCAAGG
CGTAGTGATGATCTCACCCAAGGTGCCAAGCGCAGCATTAGGCCGGGATCTGACCACTTCGATGATAGCGCTGCAATATG
CTGACGCCAGCGAACTGGCCCAGGTGTTACAGGGAGAGCAAGGGGTTGGGTTGTTGTCTGAACGCGGTGCAGTGAGCGTG
GATAAGCGCACCAATGTGATTCTAGTTCGCGACTTGGCGGACAATATAGCCGCAGCGCGTGAGATGGTGGCGATGCTTGA
TGTGCCAGTAAAGCAGGTGCAAATAGAAGCGCGTATTGTCACCATTAATCAGGGCAACTTAGAGGCGCTAGGTGTGCGTT
GGGGGATCAGTCATTCAGGGCGCCATGGTCAGGTTGGCGGCACGATTGAAGACCTTAACCTTGATTCAGCCAGCATTGAT
CAGCAGTTAAACGTCAACCTCGCTGCCCAAGCGGTTAACGCAGCGTCGATAGCTTTTCAAATCGCCAAACTCGGGTCCGA
TACCTTACTCGATCTCGAACTGTCGGCGTTGCAGCGTGAATCTAAAGCAGAGGTCATTTCAAGTCCGAGCTTGATGACCA
CACACAAGCAAGCGGCCTTCATCGAGCAAGGAACGGAGATTCCTTATCTTGAGTCATCGTCGAGCGGAGCGACCAACGTC
GCCTTCAAAAAAGCCGTATTGAGCCTTAAAGTGACGCCGCAAATCACGCCTAACAACCAGGTCATTCTTGACCTCAATGT
TACCCAAGACCGGCCCGGAGATGTGGTCAATACCGGTACCGGAGAAGCGGTGGCGATCGATACACAACGAATCGGTACCC
AAGTGTTGGTCGAAAGTGGGCAAACCATAGTACTCGGAGGGATTTTCCAACACAGCGTACTTGAAAGTGTTGATAAAGTG
CCCTTACTCGGTGATATACCGTTGCTTGGTGGCTTATTTCGCCGAACTTACCAAAAAATTGGCAAGCGTGAGCTGGTGGT
TTTTGTTACACCGAAAGTCATCACTGAGTTTAGTGAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Bacterial type II and III secretion system protein (Secretin)

(276-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

60.68

93.85

0.569
  pilQ Vibrio cholerae strain A1552

60.68

93.85

0.569
  pilQ Vibrio campbellii strain DS40M4

58.571

95.672

0.56
  pilQ Legionella pneumophila strain Lp02

41.805

95.9

0.401
  pilQ Legionella pneumophila strain ERS1305867

41.805

95.9

0.401
  comQ Acinetobacter baylyi ADP1

37.389

100

0.385
  comE Haemophilus influenzae 86-028NP

38.128

99.772

0.38
  comE Haemophilus influenzae Rd KW20

37.9

99.772

0.378
  pilQ Acinetobacter baumannii D1279779

35.622

100

0.378
  pilQ Acinetobacter baumannii strain A118

35.622

100

0.378
  pilQ Pseudomonas aeruginosa PAK

36.674

100

0.367