Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M0D44_RS16660 Genome accession   NZ_CP096138
Coordinates   3908936..3909382 (+) Length   148 a.a.
NCBI ID   WP_274704645.1    Uniprot ID   -
Organism   Xanthomonas prunicola strain MAI5084     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3908376..3917388 3908936..3909382 within 0


Gene organization within MGE regions


Location: 3908376..3917388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0D44_RS16650 (M0D44_16630) - 3908376..3908816 (+) 441 WP_260812216.1 pilin -
  M0D44_RS16660 (M0D44_16640) pilA 3908936..3909382 (+) 447 WP_274704645.1 pilin Machinery gene
  M0D44_RS16665 (M0D44_16645) pilB 3909476..3911215 (+) 1740 WP_260812217.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M0D44_RS16670 (M0D44_16650) pilR 3911749..3913203 (-) 1455 WP_207759566.1 sigma-54 dependent transcriptional regulator Regulator
  M0D44_RS16675 (M0D44_16655) - 3913474..3915087 (-) 1614 WP_101361866.1 HAMP domain-containing sensor histidine kinase -
  M0D44_RS16680 (M0D44_16660) sucC 3915319..3916488 (+) 1170 WP_101361865.1 ADP-forming succinate--CoA ligase subunit beta -
  M0D44_RS16685 (M0D44_16665) sucD 3916513..3917388 (+) 876 WP_011038208.1 succinate--CoA ligase subunit alpha -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 14869.26 Da        Isoelectric Point: 9.1337

>NTDB_id=679605 M0D44_RS16660 WP_274704645.1 3908936..3909382(+) (pilA) [Xanthomonas prunicola strain MAI5084]
MNRIQQGFTLVELMIVVAIIAILAGIALPAYQDYVVRARVSEAMVLASGLRASVITNASEGAADLSKGATLTTPLDKSPN
VVSTSISSANGVITVVTTAKAGGGTIMLTPTGAAGSALVAGVSPAGNIFWACTATIKQKYLPSSCTGN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=679605 M0D44_RS16660 WP_274704645.1 3908936..3909382(+) (pilA) [Xanthomonas prunicola strain MAI5084]
ATGAATAGGATTCAACAGGGGTTTACTCTCGTAGAGTTGATGATTGTAGTTGCAATCATCGCTATTTTGGCCGGCATTGC
CCTTCCTGCATATCAGGACTACGTAGTAAGGGCTAGAGTTTCTGAGGCTATGGTTTTAGCAAGTGGTTTGCGTGCTTCTG
TGATAACCAATGCCTCTGAAGGGGCTGCGGATCTATCGAAAGGAGCTACGCTTACGACGCCCTTAGATAAATCTCCTAAT
GTGGTTTCCACGAGTATCAGCAGCGCTAACGGTGTCATAACTGTTGTAACGACAGCAAAAGCAGGGGGGGGCACGATAAT
GCTAACGCCCACCGGGGCAGCTGGTTCGGCCTTGGTTGCAGGGGTTTCTCCTGCTGGAAATATCTTTTGGGCCTGTACTG
CCACAATTAAGCAAAAGTATTTGCCGTCTTCATGTACTGGGAATTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(36-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

45

100

0.486
  pilA2 Legionella pneumophila str. Paris

48.951

96.622

0.473
  pilA2 Legionella pneumophila strain ERS1305867

48.951

96.622

0.473
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

33.168

100

0.453
  comP Acinetobacter baylyi ADP1

40.645

100

0.426
  pilA/pilA1 Eikenella corrodens VA1

34.969

100

0.385
  pilE Neisseria gonorrhoeae MS11

33.735

100

0.378