Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M0D44_RS16665 Genome accession   NZ_CP096138
Coordinates   3909476..3911215 (+) Length   579 a.a.
NCBI ID   WP_260812217.1    Uniprot ID   -
Organism   Xanthomonas prunicola strain MAI5084     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3908376..3917388 3909476..3911215 within 0


Gene organization within MGE regions


Location: 3908376..3917388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0D44_RS16650 (M0D44_16630) - 3908376..3908816 (+) 441 WP_260812216.1 pilin -
  M0D44_RS16660 (M0D44_16640) pilA 3908936..3909382 (+) 447 WP_274704645.1 pilin Machinery gene
  M0D44_RS16665 (M0D44_16645) pilB 3909476..3911215 (+) 1740 WP_260812217.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M0D44_RS16670 (M0D44_16650) pilR 3911749..3913203 (-) 1455 WP_207759566.1 sigma-54 dependent transcriptional regulator Regulator
  M0D44_RS16675 (M0D44_16655) - 3913474..3915087 (-) 1614 WP_101361866.1 HAMP domain-containing sensor histidine kinase -
  M0D44_RS16680 (M0D44_16660) sucC 3915319..3916488 (+) 1170 WP_101361865.1 ADP-forming succinate--CoA ligase subunit beta -
  M0D44_RS16685 (M0D44_16665) sucD 3916513..3917388 (+) 876 WP_011038208.1 succinate--CoA ligase subunit alpha -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62804.88 Da        Isoelectric Point: 5.6024

>NTDB_id=679606 M0D44_RS16665 WP_260812217.1 3909476..3911215(+) (pilB) [Xanthomonas prunicola strain MAI5084]
MNQVSSSANLVGITGIARRLVQDGALEEAAARIAMEQATAAKVPLPQWFSDKKLVSGAQLAAANAVEFGMPLMDVSAFDA
NQNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASFGSSL
GNDDDEMGDLEVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKA
PVKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQ
KLFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQD
PDIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKASL
PEHALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSAL
MKAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=679606 M0D44_RS16665 WP_260812217.1 3909476..3911215(+) (pilB) [Xanthomonas prunicola strain MAI5084]
GTGAACCAAGTATCAAGTAGCGCTAATTTGGTCGGCATCACAGGTATAGCCCGTCGGCTTGTCCAAGATGGGGCCTTAGA
AGAAGCTGCTGCTCGTATTGCGATGGAGCAGGCTACTGCAGCTAAAGTTCCGTTGCCACAATGGTTTTCTGATAAAAAAC
TAGTCTCCGGGGCTCAACTCGCTGCAGCCAACGCCGTTGAGTTCGGGATGCCGCTGATGGATGTGTCGGCGTTCGACGCC
AATCAGAACGCGGTCAAGCTGGTCAGTGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCG
GTTGTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGATATCAAGTTCCATACGAACCTGGTTGTCGAGC
CTATCCTGGTCGACGAGGACCAGATTCGTCGCACTTTGGAGCAGTGGCAGGCAAGTAACGCTTCGTTTGGTTCGTCGTTG
GGGAACGACGATGACGAGATGGGGGACCTGGAGGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGT
CGATGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCG
ACATCCATTTCGAGCCCTACGAAGACGATTACCGGGTGCGCTTGCGCATCGACGGCCTGCTGAAGAATGTGGCCAAGGCG
CCGGTGAAGCTGAGTCAGCGCATTGCCGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCA
GGACGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACCCTGTTCGGCG
AGAAGGTGGTGCTGCGTATTCTGGACGGCAGCGCAGCCAAGCTGGGCATCGAGAAGCTGGGCTATGAGGCGGACCAGCAG
AAGCTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCCGGCAAGACGGTGTC
GCTGTATACGGCCTTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACGGCCGAGGATCCGGTGGAAATCCGCCTGC
CCGGCGTCAATCAGGTGCAGCAGAACAACAAGCGCGGCATGACGTTTGCCGCGGCCTTGCGCTCGTTCCTGCGCCAGGAC
CCGGACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACAT
GGTGCTATCGACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCTTGATGAACATGGGCATTGCGCCGTACAACA
TCACTTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGTCGGTTGTGCAACAACTGCAAGCGCAAGGCGAGCCTG
CCCGAGCATGCGTTGCTGGCCGAAGGCTTCACGCCGGCGCAGATCGCTGCCGGTATCGAACTGTACGAGGCGGTCGGTTG
CGACGAGTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCG
TGCTGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAAAGTATCGGCATCCGTGATCTGCGCCAGTCGGCCTTG
ATGAAGGCCGCGCATGGGGTGACCAGCCTGGCGGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.634

98.1

0.546
  pilB Acinetobacter baumannii D1279779

55.282

98.1

0.542
  pilB Legionella pneumophila strain ERS1305867

52.373

98.273

0.515
  pilB Vibrio cholerae strain A1552

48.601

98.791

0.48
  pilF Neisseria gonorrhoeae MS11

48.768

98.1

0.478
  pilB Vibrio campbellii strain DS40M4

45.87

98.273

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

46.429

96.718

0.449