Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MYF86_RS07110 Genome accession   NZ_CP095841
Coordinates   1493792..1494901 (+) Length   369 a.a.
NCBI ID   WP_005340356.1    Uniprot ID   -
Organism   Aeromonas veronii strain AV040     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1488792..1499901
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYF86_RS07080 (MYF86_07080) - 1489694..1490113 (-) 420 WP_005340339.1 DUF4426 domain-containing protein -
  MYF86_RS07085 (MYF86_07085) yggU 1490157..1490459 (-) 303 WP_005340341.1 DUF167 family protein YggU -
  MYF86_RS07090 (MYF86_07090) - 1490459..1491010 (-) 552 WP_005340343.1 YggT family protein -
  MYF86_RS07095 (MYF86_07095) proC 1491022..1491846 (-) 825 WP_042053316.1 pyrroline-5-carboxylate reductase -
  MYF86_RS07100 (MYF86_07100) - 1491988..1492689 (-) 702 WP_058057968.1 YggS family pyridoxal phosphate-dependent enzyme -
  MYF86_RS07105 (MYF86_07105) pilT 1492729..1493763 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  MYF86_RS07110 (MYF86_07110) pilU 1493792..1494901 (+) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  MYF86_RS07115 (MYF86_07115) yaaA 1494949..1495722 (+) 774 WP_236397343.1 peroxide stress protein YaaA -
  MYF86_RS07120 (MYF86_07120) - 1495917..1496342 (+) 426 WP_247823898.1 hypothetical protein -
  MYF86_RS07125 (MYF86_07125) - 1496703..1497194 (-) 492 WP_021230946.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  MYF86_RS07130 (MYF86_07130) arfB 1497227..1497643 (-) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  MYF86_RS07135 (MYF86_07135) glnK 1497845..1498183 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  MYF86_RS07140 (MYF86_07140) - 1498442..1499449 (+) 1008 WP_247824166.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41076.12 Da        Isoelectric Point: 6.5043

>NTDB_id=678695 MYF86_RS07110 WP_005340356.1 1493792..1494901(+) (pilU) [Aeromonas veronii strain AV040]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=678695 MYF86_RS07110 WP_005340356.1 1493792..1494901(+) (pilU) [Aeromonas veronii strain AV040]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTTGTGTCGCTGGGGGGGGAGGCGCTCGACAAGAAGGGGGCACTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAAGCGCTTGGCCGT
TTTCGGGTCAGTGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACTACTCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCATTCGATGT
GGCGCTAAAAAGCTCGCTGCGTCAGGCACCGGACGTGATCCTGATCGGCGAGATCCGCAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGTTGGATCGTATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCTATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAGGCG
CTTTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAACGGCTCGGCACCGGTACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36