Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   LGT42_RS14420 Genome accession   NZ_CP095733
Coordinates   2984764..2986011 (+) Length   415 a.a.
NCBI ID   WP_226072920.1    Uniprot ID   -
Organism   Methylophaga pinxianii strain TMB1265     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2979764..2991011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGT42_RS14395 (LGT42_014395) - 2979930..2980640 (-) 711 WP_226072928.1 DUF484 family protein -
  LGT42_RS14400 (LGT42_014400) dapF 2980616..2981464 (-) 849 WP_319000263.1 diaminopimelate epimerase -
  LGT42_RS14405 (LGT42_014405) lysA 2981469..2982722 (-) 1254 WP_226072926.1 diaminopimelate decarboxylase -
  LGT42_RS14410 (LGT42_014410) - 2982727..2982855 (-) 129 WP_226072924.1 lipoprotein -
  LGT42_RS14415 (LGT42_014415) pilB 2983042..2984760 (+) 1719 WP_226072922.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LGT42_RS14420 (LGT42_014420) pilC 2984764..2986011 (+) 1248 WP_226072920.1 type II secretion system F family protein Machinery gene
  LGT42_RS14425 (LGT42_014425) pilD 2986016..2986885 (+) 870 WP_226072910.1 A24 family peptidase Machinery gene
  LGT42_RS14430 (LGT42_014430) coaE 2986887..2987495 (+) 609 WP_226072902.1 dephospho-CoA kinase -
  LGT42_RS14435 (LGT42_014435) zapD 2987539..2988309 (+) 771 WP_226072900.1 cell division protein ZapD -
  LGT42_RS14440 (LGT42_014440) - 2988312..2988497 (+) 186 WP_226073225.1 DNA gyrase inhibitor YacG -
  LGT42_RS14445 (LGT42_014445) mutT 2988494..2988895 (-) 402 WP_226072898.1 8-oxo-dGTP diphosphatase MutT -
  LGT42_RS14450 (LGT42_014450) - 2988899..2989084 (-) 186 WP_226072896.1 zinc-finger domain-containing protein -
  LGT42_RS14455 (LGT42_014455) nadA 2989103..2990215 (-) 1113 WP_226072895.1 quinolinate synthase NadA -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 45573.90 Da        Isoelectric Point: 9.8655

>NTDB_id=677802 LGT42_RS14420 WP_226072920.1 2984764..2986011(+) (pilC) [Methylophaga pinxianii strain TMB1265]
MATASKAKLKPKKAELPTLYIWQGTNKAGRKVKGEQAGESVQAVRADLRRQGITPGKVRRKPKDLFAPRKPKIKPVHIAI
FSRMLATMMSSGVPMMQSLQIIGEGHENASMQEMILSIKADVESGTSLAESLAKFPLHFDDLYISLINAGEQSGTLEALL
HEIATYQEKTEALKSKIKKALVYPTAIMVVAFIVTAILMIFVIPQFQALFTGFGADLPGLTKMVISISQVFQDYWWLIFG
TIIGSVMGFMAARRRSRKIQHFMDRMLLKLPVIGDVLTKGAIARFARTFSVMFKAGVPMVEAMTSVAGATGNIVYNEATL
EMRDDVATGTQLNKAMTDTELFPNMVIQMVSIGEESGSLDAMLAKVADFYEREVDDAVDNMTALMEPFIMAFLGVLIGTL
VIAMYLPIFKLGAVV

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=677802 LGT42_RS14420 WP_226072920.1 2984764..2986011(+) (pilC) [Methylophaga pinxianii strain TMB1265]
GTGGCCACAGCAAGTAAAGCGAAACTCAAACCTAAAAAAGCTGAATTACCCACACTTTACATATGGCAAGGCACCAATAA
AGCTGGCCGTAAGGTAAAAGGTGAGCAGGCTGGTGAGAGTGTTCAAGCTGTGCGAGCAGATTTACGTCGTCAAGGTATTA
CGCCGGGGAAAGTACGTCGTAAACCCAAAGATTTATTTGCCCCTCGTAAACCCAAGATAAAACCCGTCCATATCGCGATT
TTCAGTCGTATGTTGGCGACCATGATGTCTTCCGGTGTGCCGATGATGCAATCTCTGCAAATCATTGGTGAAGGCCATGA
AAACGCCAGTATGCAGGAAATGATTCTATCGATTAAAGCTGATGTGGAATCAGGTACCAGTCTGGCAGAATCGTTAGCCA
AATTCCCGTTACATTTTGATGATCTGTATATCAGCCTTATTAATGCCGGCGAACAATCCGGTACCTTGGAAGCACTGTTA
CACGAAATTGCTACCTATCAGGAAAAAACTGAAGCGCTGAAGTCCAAAATCAAAAAAGCACTGGTTTATCCTACTGCCAT
TATGGTGGTGGCTTTTATCGTCACCGCGATTTTAATGATATTTGTTATTCCCCAGTTTCAGGCGTTATTTACCGGCTTTG
GAGCGGACTTACCCGGCCTGACCAAAATGGTTATCAGTATTTCCCAGGTCTTTCAGGATTACTGGTGGTTAATTTTTGGC
ACGATAATCGGTTCAGTCATGGGGTTTATGGCTGCGCGCAGACGTTCACGCAAGATTCAACATTTTATGGATCGGATGCT
ATTAAAACTGCCGGTTATCGGTGACGTATTGACCAAAGGCGCGATTGCACGATTTGCCAGAACATTTTCGGTCATGTTTA
AAGCCGGTGTCCCCATGGTGGAAGCCATGACCTCAGTGGCTGGTGCCACTGGCAACATTGTCTATAACGAAGCCACACTT
GAAATGCGTGATGATGTGGCTACCGGTACCCAATTAAACAAAGCAATGACTGATACCGAGTTATTTCCAAATATGGTCAT
TCAGATGGTATCGATTGGTGAAGAATCCGGTTCGCTGGATGCGATGCTGGCAAAAGTAGCTGATTTCTATGAACGTGAAG
TGGATGATGCCGTAGACAACATGACAGCATTAATGGAGCCTTTTATTATGGCTTTCCTGGGAGTATTAATCGGTACGTTG
GTTATCGCCATGTACTTGCCGATATTTAAACTCGGCGCAGTCGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(81-204)

Type II secretion system (T2SS), protein F (T2SSF)

(285-407)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.455

97.349

0.53
  pilC Legionella pneumophila strain ERS1305867

55.076

94.94

0.523
  pilC Acinetobacter baylyi ADP1

50.37

97.59

0.492
  pilC Acinetobacter baumannii D1279779

49.383

97.59

0.482
  pilG Neisseria gonorrhoeae MS11

44.836

95.663

0.429
  pilG Neisseria meningitidis 44/76-A

44.584

95.663

0.427
  pilC Vibrio cholerae strain A1552

41.919

95.422

0.4
  pilC Vibrio campbellii strain DS40M4

39.899

95.422

0.381