Detailed information NTG0677803

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: LGT42_RS14425
• Genome accession: NZ_CP095733
• Coordinates: 2986016..2986885 (+)
• Length: 289 a.a.
• NCBI ID: WP_226072910.1
• Uniprot ID: -
• Organism: Methylophaga pinxianii strain TMB1265
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2981016..2991885

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LGT42_RS14405 (LGT42_014405)	lysA	2981469..2982722 (-)	1254	WP_226072926.1	diaminopimelate decarboxylase	-
LGT42_RS14410 (LGT42_014410)	-	2982727..2982855 (-)	129	WP_226072924.1	lipoprotein	-
LGT42_RS14415 (LGT42_014415)	pilB	2983042..2984760 (+)	1719	WP_226072922.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LGT42_RS14420 (LGT42_014420)	pilC	2984764..2986011 (+)	1248	WP_226072920.1	type II secretion system F family protein	Machinery gene
LGT42_RS14425 (LGT42_014425)	pilD	2986016..2986885 (+)	870	WP_226072910.1	A24 family peptidase	Machinery gene
LGT42_RS14430 (LGT42_014430)	coaE	2986887..2987495 (+)	609	WP_226072902.1	dephospho-CoA kinase	-
LGT42_RS14435 (LGT42_014435)	zapD	2987539..2988309 (+)	771	WP_226072900.1	cell division protein ZapD	-
LGT42_RS14440 (LGT42_014440)	-	2988312..2988497 (+)	186	WP_226073225.1	DNA gyrase inhibitor YacG	-
LGT42_RS14445 (LGT42_014445)	mutT	2988494..2988895 (-)	402	WP_226072898.1	8-oxo-dGTP diphosphatase MutT	-
LGT42_RS14450 (LGT42_014450)	-	2988899..2989084 (-)	186	WP_226072896.1	zinc-finger domain-containing protein	-
LGT42_RS14455 (LGT42_014455)	nadA	2989103..2990215 (-)	1113	WP_226072895.1	quinolinate synthase NadA	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31624.46 Da
• Isoelectric Point: 6.8233

>NTDB_id=677803 LGT42_RS14425 WP_226072910.1 2986016..2986885(+) (pilD) [Methylophaga pinxianii strain TMB1265]
MSLIYLLETSSAFFIASIFILGLLVGSFLNVVIHRLPLMMQREWAQQCNQLIGTAEPETDELTLSTPRSRCPHCGHAITA
LENIPVLSYLALKGRCRECKAPISKRYPLIELFSAIISAVIAWHFGFGVAVIGALLLSWALLALTFIDVDHQLLPDSITL
PLLWLGLIFNLFGTFTDINSAVTGAIAGYLALWLVYHAFRLATGKEGMGYGDFKLLAALGAWMGWQALPMIILLSSLVGA
LIGISLILLKQQHRDIPIPFGPYLAAAGWLALVWGESLTQGYLNWSGLS

Nucleotide

• Length: 870 bp

>NTDB_id=677803 LGT42_RS14425 WP_226072910.1 2986016..2986885(+) (pilD) [Methylophaga pinxianii strain TMB1265]
ATGTCGCTTATTTATCTGCTGGAAACATCCAGTGCTTTTTTTATTGCCAGTATTTTTATTCTGGGGTTACTGGTGGGCAG
TTTTCTCAATGTGGTGATTCACCGCCTGCCACTGATGATGCAACGCGAATGGGCTCAACAATGCAATCAGTTAATTGGAA
CCGCAGAACCCGAGACAGATGAACTGACATTAAGTACGCCTCGCTCGCGCTGTCCACATTGTGGTCATGCCATTACGGCT
CTGGAAAATATTCCGGTACTCAGCTATCTGGCGCTAAAAGGTCGTTGCCGTGAATGCAAAGCACCGATCTCCAAACGTTA
TCCTCTTATCGAACTATTCTCCGCGATAATATCGGCCGTTATCGCATGGCACTTTGGTTTCGGTGTGGCGGTTATAGGTG
CATTATTACTGAGCTGGGCTTTACTTGCTTTGACCTTTATTGATGTGGACCACCAGTTGTTACCAGATAGTATTACTCTG
CCCCTATTATGGTTAGGGCTGATCTTCAATCTGTTTGGTACATTTACCGATATCAACTCTGCTGTGACGGGGGCTATCGC
AGGTTATCTCGCGCTCTGGTTGGTGTATCATGCCTTTCGCCTTGCTACCGGAAAGGAAGGTATGGGTTATGGTGATTTCA
AACTTCTGGCAGCCTTGGGCGCCTGGATGGGATGGCAGGCACTTCCCATGATTATTTTGCTGTCATCTCTGGTCGGAGCA
CTGATTGGGATTAGTTTAATTTTGTTGAAACAACAGCACCGCGACATCCCCATTCCTTTTGGGCCATATCTGGCAGCCGC
TGGCTGGTTAGCACTGGTTTGGGGTGAAAGCCTGACTCAGGGATACCTCAATTGGAGTGGATTAAGCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	57.455	95.156	0.547
pilD	Vibrio campbellii strain DS40M4	52.727	95.156	0.502
pilD	Acinetobacter baumannii D1279779	51.601	97.232	0.502
pilD	Acinetobacter nosocomialis M2	51.246	97.232	0.498
pilD	Neisseria gonorrhoeae MS11	46.975	97.232	0.457