Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LGT42_RS14415 Genome accession   NZ_CP095733
Coordinates   2983042..2984760 (+) Length   572 a.a.
NCBI ID   WP_226072922.1    Uniprot ID   -
Organism   Methylophaga pinxianii strain TMB1265     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2978042..2989760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGT42_RS14385 (LGT42_014385) - 2978364..2978573 (-) 210 WP_247363586.1 hypothetical protein -
  LGT42_RS14390 (LGT42_014390) xerC 2979032..2979940 (-) 909 WP_226072930.1 tyrosine recombinase XerC -
  LGT42_RS14395 (LGT42_014395) - 2979930..2980640 (-) 711 WP_226072928.1 DUF484 family protein -
  LGT42_RS14400 (LGT42_014400) dapF 2980616..2981464 (-) 849 WP_319000263.1 diaminopimelate epimerase -
  LGT42_RS14405 (LGT42_014405) lysA 2981469..2982722 (-) 1254 WP_226072926.1 diaminopimelate decarboxylase -
  LGT42_RS14410 (LGT42_014410) - 2982727..2982855 (-) 129 WP_226072924.1 lipoprotein -
  LGT42_RS14415 (LGT42_014415) pilB 2983042..2984760 (+) 1719 WP_226072922.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LGT42_RS14420 (LGT42_014420) pilC 2984764..2986011 (+) 1248 WP_226072920.1 type II secretion system F family protein Machinery gene
  LGT42_RS14425 (LGT42_014425) pilD 2986016..2986885 (+) 870 WP_226072910.1 A24 family peptidase Machinery gene
  LGT42_RS14430 (LGT42_014430) coaE 2986887..2987495 (+) 609 WP_226072902.1 dephospho-CoA kinase -
  LGT42_RS14435 (LGT42_014435) zapD 2987539..2988309 (+) 771 WP_226072900.1 cell division protein ZapD -
  LGT42_RS14440 (LGT42_014440) - 2988312..2988497 (+) 186 WP_226073225.1 DNA gyrase inhibitor YacG -
  LGT42_RS14445 (LGT42_014445) mutT 2988494..2988895 (-) 402 WP_226072898.1 8-oxo-dGTP diphosphatase MutT -
  LGT42_RS14450 (LGT42_014450) - 2988899..2989084 (-) 186 WP_226072896.1 zinc-finger domain-containing protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62904.15 Da        Isoelectric Point: 5.2229

>NTDB_id=677801 LGT42_RS14415 WP_226072922.1 2983042..2984760(+) (pilB) [Methylophaga pinxianii strain TMB1265]
MANMTAPIRLSGLARRLVSEGLLSEDQAQAAQATAKQASQSFVTYLVNNKLLKSDVIGAIASQEFGVPLFDLDSMNMELA
PTSLFQEKLIRQHNALPLFQRGKRLYVAVSDPTNLQALDEFQFNTALNTYPVLVDEQKLSVTIEKALDNKNQVLDEFDDA
ELDDIDLGPDDDADRAAELSEKNIDDTPVVRFINGILTQAIKAGASDIHFEPYEKKYRVRVRIDGILHETKTAPVALGGR
IAARLKVLSRMNIAERRVPQDGRMRLKVSKSKAIDFRVSTCPTLFGEKIVLRILDPTSAQLGIDALGYEEDQKKIFLETM
HKPYGMVLVTGPTGSGKTVSLYTALNILNTDDRNISTAEDPAEINLPGINQVNVHPQIGLGFTECLRAFLRQDPDVIMVG
EIRDLETAEIAIKAAQTGHMVFSTLHTNDAPQTLTRLANMGVPAFNIASAVTLIIAQRLARRLCPHCKAVEPLPRETLIE
EGYTPEQIATDFKVYRAVGCEQCTEGYKGRVGIYQVMPVSDAMGRLIMEGGNSMQLADQAKAEGIADLRASGLKKVMAGL
TSLEEINRVIKE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=677801 LGT42_RS14415 WP_226072922.1 2983042..2984760(+) (pilB) [Methylophaga pinxianii strain TMB1265]
ATGGCAAATATGACAGCACCAATCAGATTAAGCGGTCTGGCGAGGCGCCTTGTGAGTGAAGGGTTGCTGAGTGAAGACCA
GGCCCAAGCGGCTCAAGCAACGGCTAAACAGGCTTCTCAATCCTTTGTAACCTATCTCGTAAACAATAAATTACTTAAGA
GTGATGTCATCGGTGCGATTGCCTCTCAGGAATTTGGCGTACCGCTGTTTGACCTCGACAGCATGAATATGGAACTGGCA
CCAACCAGTCTGTTTCAGGAAAAACTGATCCGACAACATAACGCCCTGCCATTATTTCAACGTGGCAAACGCCTGTATGT
GGCCGTATCCGATCCAACGAACTTGCAAGCGCTGGATGAGTTCCAGTTCAACACCGCACTAAATACCTACCCTGTTCTGG
TTGATGAACAAAAACTCTCCGTCACTATCGAAAAGGCTCTTGATAATAAAAATCAGGTATTGGATGAGTTCGACGATGCT
GAGCTGGACGATATTGATTTAGGGCCAGATGATGATGCGGATCGTGCCGCCGAGTTGTCTGAGAAAAATATTGATGATAC
GCCGGTTGTTCGGTTTATCAACGGCATCCTGACGCAGGCGATTAAAGCTGGTGCCTCGGATATTCACTTTGAACCCTATG
AGAAAAAATACCGTGTCAGGGTACGTATAGACGGTATTTTGCATGAAACTAAAACAGCTCCCGTGGCATTAGGTGGTCGG
ATTGCGGCCCGTTTGAAAGTTCTGTCTCGGATGAACATCGCAGAACGCCGGGTGCCTCAGGACGGCCGGATGCGGCTGAA
GGTGTCCAAATCCAAAGCTATCGATTTTCGTGTTAGTACCTGTCCGACGTTGTTCGGCGAAAAGATCGTCCTACGTATTC
TCGATCCCACCAGTGCCCAATTGGGTATTGATGCCTTGGGATATGAAGAAGATCAGAAAAAAATCTTCCTGGAAACCATG
CACAAACCTTATGGCATGGTGCTCGTCACGGGCCCGACGGGAAGTGGTAAAACGGTTTCGTTATATACCGCGTTGAATAT
TCTGAATACCGATGACCGCAATATTTCAACCGCAGAAGATCCGGCAGAGATCAATCTGCCAGGTATCAACCAGGTCAATG
TGCATCCACAAATTGGTTTGGGCTTTACTGAATGTTTAAGAGCCTTCTTACGTCAGGATCCTGACGTTATCATGGTCGGT
GAGATCCGCGATCTGGAAACGGCTGAAATTGCTATCAAAGCTGCGCAGACAGGACATATGGTGTTTTCAACATTACATAC
CAATGATGCTCCGCAAACGCTTACCCGTCTCGCAAATATGGGGGTTCCGGCCTTTAATATTGCTTCTGCTGTCACGTTGA
TTATTGCCCAGCGTCTGGCGCGTCGTTTGTGCCCACATTGCAAGGCCGTCGAGCCATTACCGAGGGAAACGCTGATTGAA
GAGGGTTACACTCCCGAACAAATTGCGACAGATTTCAAAGTTTACCGTGCTGTAGGTTGCGAACAATGTACCGAGGGGTA
TAAAGGTCGGGTAGGTATCTATCAGGTTATGCCCGTTTCCGATGCCATGGGACGTTTGATCATGGAAGGTGGAAACTCGA
TGCAATTAGCTGATCAGGCTAAAGCTGAAGGTATTGCCGATCTACGGGCTTCCGGTCTGAAAAAGGTAATGGCCGGTTTA
ACCAGTCTGGAAGAAATTAACCGCGTTATTAAGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.567

99.825

0.565
  pilB Acinetobacter baylyi ADP1

55.323

100

0.554
  pilB Legionella pneumophila strain ERS1305867

53.927

100

0.54
  pilB Vibrio parahaemolyticus RIMD 2210633

50.088

99.126

0.497
  pilB Vibrio cholerae strain A1552

49.472

99.301

0.491
  pilB Vibrio campbellii strain DS40M4

48.415

99.301

0.481
  pilF Neisseria gonorrhoeae MS11

48.566

97.552

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

36.851

100

0.397
  pilF Thermus thermophilus HB27

37.676

99.301

0.374