Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MRK30_RS01635 Genome accession   NZ_CP095357
Coordinates   341225..341662 (+) Length   145 a.a.
NCBI ID   WP_004789794.1    Uniprot ID   A0A009QRP9
Organism   Acinetobacter pittii strain 19MO01SH04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 336225..346662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRK30_RS01615 (MRK30_01620) - 337607..338494 (+) 888 WP_014207824.1 metal-dependent hydrolase -
  MRK30_RS01620 (MRK30_01625) - 338625..339215 (+) 591 WP_002117090.1 LemA family protein -
  MRK30_RS01625 (MRK30_01630) - 339240..340322 (+) 1083 WP_032063182.1 TPM domain-containing protein -
  MRK30_RS01630 (MRK30_01635) - 340316..340876 (+) 561 WP_032057259.1 TPM domain-containing protein -
  MRK30_RS01635 (MRK30_01640) pilA 341225..341662 (+) 438 WP_004789794.1 pilin Machinery gene
  MRK30_RS01640 (MRK30_01645) - 341720..343033 (+) 1314 WP_199953047.1 O-antigen ligase family protein -
  MRK30_RS01645 (MRK30_01650) - 343139..344767 (+) 1629 WP_017481721.1 O-antigen ligase family protein -
  MRK30_RS01650 (MRK30_01655) bfr 344810..345274 (-) 465 WP_005067113.1 bacterioferritin -
  MRK30_RS01655 (MRK30_01660) bfd 345518..345712 (-) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  MRK30_RS01660 (MRK30_01665) - 345815..345915 (+) 101 Protein_318 hypothetical protein -
  MRK30_RS01665 (MRK30_01670) - 345932..346315 (-) 384 WP_002116996.1 RidA family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14801.82 Da        Isoelectric Point: 8.1114

>NTDB_id=676466 MRK30_RS01635 WP_004789794.1 341225..341662(+) (pilA) [Acinetobacter pittii strain 19MO01SH04]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIAKSQVGTGLTDITAGKTNTETKLAEGLAANLSAASDVGLQSSSNAC
SSISVSVATTGLTTIECTLQGTSQINGKKISWERTADSATAGTTGTWRCKTDVATNLRPKSCGAS

Nucleotide


Download         Length: 438 bp        

>NTDB_id=676466 MRK30_RS01635 WP_004789794.1 341225..341662(+) (pilA) [Acinetobacter pittii strain 19MO01SH04]
ATGAATGCACAAAAAGGTTTTACATTAATTGAGCTTATGATCGTGGTTGCCATTATTGGTATTTTGGCAGCAATTGCGAT
TCCTGCATATCAAAACTATATTGCTAAGTCTCAAGTAGGAACAGGTTTAACCGATATTACAGCTGGTAAAACTAATACAG
AAACTAAATTAGCAGAAGGTTTAGCAGCTAATTTATCTGCAGCATCTGACGTAGGACTACAAAGCTCGAGTAATGCTTGT
TCTTCTATTTCAGTATCTGTTGCTACTACTGGATTGACAACAATTGAATGTACATTGCAAGGTACTTCACAAATCAACGG
TAAGAAAATTTCTTGGGAACGAACAGCTGATAGTGCTACAGCAGGCACTACGGGTACATGGCGTTGTAAAACTGATGTTG
CAACAAATTTACGTCCTAAATCATGTGGTGCTTCGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009QRP9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

50.34

100

0.51
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.497

100

0.421
  pilA Vibrio cholerae strain A1552

41.497

100

0.421
  pilA Vibrio cholerae C6706

41.497

100

0.421
  comP Acinetobacter baylyi ADP1

40.397

100

0.421
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.958

98.621

0.414
  pilA Pseudomonas aeruginosa PAK

38.961

100

0.414
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.549

97.931

0.407
  pilA2 Legionella pneumophila strain ERS1305867

38.667

100

0.4