Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MRO13_RS02400 Genome accession   NZ_CP095311
Coordinates   502485..504173 (-) Length   562 a.a.
NCBI ID   WP_350940065.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain 20CO01TI02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 497485..509173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRO13_RS02375 (MRO13_02375) yacG 498573..498770 (-) 198 WP_040903818.1 DNA gyrase inhibitor YacG -
  MRO13_RS02380 (MRO13_02380) zapD 498812..499552 (-) 741 WP_004394266.1 cell division protein ZapD -
  MRO13_RS02385 (MRO13_02385) coaE 499584..500192 (-) 609 WP_158138885.1 dephospho-CoA kinase -
  MRO13_RS02390 (MRO13_02390) pilD 500198..501064 (-) 867 WP_158138884.1 A24 family peptidase Machinery gene
  MRO13_RS02395 (MRO13_02395) pilC 501240..502466 (-) 1227 WP_154170154.1 type II secretion system F family protein Machinery gene
  MRO13_RS02400 (MRO13_02400) pilB 502485..504173 (-) 1689 WP_350940065.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MRO13_RS02405 (MRO13_02405) pilA 504185..504640 (-) 456 WP_350940067.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MRO13_RS02410 (MRO13_02410) nadC 504923..505813 (-) 891 WP_350940069.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MRO13_RS02415 (MRO13_02415) ampD 505922..506467 (+) 546 WP_154170162.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MRO13_RS02420 (MRO13_02420) fldB 506464..506982 (-) 519 WP_350940071.1 flavodoxin FldB -
  MRO13_RS02425 (MRO13_02425) xerD 507168..508022 (+) 855 WP_233268213.1 site-specific tyrosine recombinase XerD -
  MRO13_RS02430 (MRO13_02430) - 508038..508790 (+) 753 WP_170906300.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62036.12 Da        Isoelectric Point: 5.0074

>NTDB_id=676280 MRO13_RS02400 WP_350940065.1 502485..504173(-) (pilB) [Vibrio metschnikovii strain 20CO01TI02]
MLTNLIAILRQAQLLSLTQEKALVEHIQASGASAPEALLDLAIFDSQELSEHISQIFALPLQNLNDYAYPELCQQLGLRE
LIMRYQALPIENNGLMLTLAMADPTHLQAEEEFRFATGLAIEVVLADYKALQGAIRRLYGRSLAGHSAQGKEISQDELAS
LVDISDDEQQQIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESLYRVRLRCDGILVQTQQPASHLGRRLAARIKI
LAKLDIAERRLPQDGRIKLRLNATTAIDMRVSTLPTLWGEKIVLRLLDSSAASLDIDKLGYNEVQKQLYLKALQQPQGMI
LMTGPTGSGKTVSLYTGLSLLNREQVNISTAEDPVEINLPGINQVQVQPKIGFGFAKALRSFLRQDPDIVMVGEIRDVET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLTNMGIEPFNLASSLSLIIAQRLARRLCPNCKVAKQPNVTEQQQFDLSADD
ILYAADPNGCSECTKGYSGRVGIYEVMAFNSQLAEAIMQRASITHIEAIAKANGMQTLQESGIEKLQQGITSIDELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=676280 MRO13_RS02400 WP_350940065.1 502485..504173(-) (pilB) [Vibrio metschnikovii strain 20CO01TI02]
ATGCTCACCAATCTCATCGCGATTTTGCGTCAGGCGCAATTACTTAGCCTGACCCAAGAAAAAGCGCTGGTCGAACATAT
CCAAGCTTCTGGTGCCAGTGCGCCGGAAGCTTTGCTGGATTTGGCGATCTTCGATAGCCAAGAGCTGAGCGAACACATCA
GTCAGATATTCGCTTTACCGCTCCAAAATCTTAACGATTATGCCTACCCAGAGCTGTGCCAACAGCTCGGATTACGTGAG
TTAATCATGCGCTACCAAGCGCTACCGATTGAGAATAATGGTTTGATGCTCACCTTAGCCATGGCCGATCCTACTCATCT
GCAAGCAGAAGAAGAATTTCGCTTTGCCACCGGACTCGCGATTGAAGTGGTGTTGGCAGACTATAAAGCACTACAAGGGG
CTATTCGCCGCTTATACGGACGTTCATTAGCTGGGCATTCTGCGCAAGGCAAAGAGATCAGCCAAGACGAGCTAGCCAGC
TTAGTCGATATTTCTGATGATGAGCAGCAACAAATCGAGGATCTTAGCCAAGATGACTCGCCCGTTAGCCGCTTTATCAA
CCAAATTTTGCTTGATGCGGTGCGTAAAGGTGCGTCCGATATTCACTTTGAACCTTACGAGTCCCTTTATCGAGTTCGCT
TACGCTGCGATGGTATTTTGGTGCAAACCCAACAACCCGCCAGCCATTTAGGGCGGCGACTGGCAGCGCGGATTAAAATT
TTAGCGAAACTGGATATCGCCGAGCGCCGCTTGCCACAAGACGGGCGAATTAAATTGCGCCTCAATGCCACCACCGCGAT
TGATATGCGCGTTTCCACCTTACCCACCTTATGGGGAGAAAAAATTGTCCTACGCTTACTCGATAGCAGCGCCGCCAGTT
TAGATATCGATAAATTAGGTTATAACGAGGTGCAAAAGCAGCTCTATCTCAAGGCGCTTCAGCAGCCACAAGGGATGATT
TTAATGACCGGCCCGACAGGTAGCGGAAAAACCGTTTCCCTTTATACTGGGCTGAGTTTATTAAATCGTGAGCAGGTGAA
TATTTCTACCGCCGAAGATCCCGTCGAAATCAATCTCCCCGGCATCAATCAGGTACAAGTGCAACCTAAAATCGGTTTTG
GCTTTGCTAAGGCGCTGCGTTCTTTTTTACGCCAAGATCCGGACATTGTTATGGTCGGTGAAATCCGTGATGTCGAGACC
GCTGAAATTGCCGTTAAAGCTGCTCAAACCGGGCATTTAGTGCTCTCCACGTTACACACTAACTCAGCAGCAGAGACCGT
GATTCGCCTGACTAATATGGGCATTGAACCTTTTAATCTCGCTTCTTCATTAAGCTTAATCATCGCTCAGCGCTTAGCAC
GCCGTTTATGTCCGAACTGTAAAGTCGCCAAACAACCAAACGTAACGGAACAACAGCAGTTTGACTTATCAGCCGATGAC
ATTCTCTACGCGGCGGATCCGAACGGTTGCAGTGAATGCACCAAAGGCTACAGTGGCCGAGTCGGCATTTATGAAGTGAT
GGCGTTTAATAGCCAATTAGCTGAAGCGATCATGCAGCGCGCTTCCATTACTCATATCGAAGCCATTGCTAAAGCTAATG
GTATGCAAACTTTACAAGAATCTGGAATAGAAAAACTCCAACAGGGGATCACCAGCATTGACGAGCTGCAGCGTGTTCTT
TACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

79.181

100

0.792
  pilB Vibrio campbellii strain DS40M4

71.352

100

0.714
  pilB Vibrio parahaemolyticus RIMD 2210633

71.352

100

0.714
  pilB Legionella pneumophila strain ERS1305867

50.947

93.95

0.479
  pilB Acinetobacter baumannii D1279779

50.289

92.349

0.464
  pilF Neisseria gonorrhoeae MS11

44.504

100

0.447
  pilB Acinetobacter baylyi ADP1

48.643

91.815

0.447
  pilB/pilB1 Synechocystis sp. PCC 6803

38.063

100

0.406
  pilF Thermus thermophilus HB27

39.372

96.263

0.379