Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MRO13_RS02405 Genome accession   NZ_CP095311
Coordinates   504185..504640 (-) Length   151 a.a.
NCBI ID   WP_350940067.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain 20CO01TI02     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 499185..509640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRO13_RS02385 (MRO13_02385) coaE 499584..500192 (-) 609 WP_158138885.1 dephospho-CoA kinase -
  MRO13_RS02390 (MRO13_02390) pilD 500198..501064 (-) 867 WP_158138884.1 A24 family peptidase Machinery gene
  MRO13_RS02395 (MRO13_02395) pilC 501240..502466 (-) 1227 WP_154170154.1 type II secretion system F family protein Machinery gene
  MRO13_RS02400 (MRO13_02400) pilB 502485..504173 (-) 1689 WP_350940065.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MRO13_RS02405 (MRO13_02405) pilA 504185..504640 (-) 456 WP_350940067.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MRO13_RS02410 (MRO13_02410) nadC 504923..505813 (-) 891 WP_350940069.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MRO13_RS02415 (MRO13_02415) ampD 505922..506467 (+) 546 WP_154170162.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MRO13_RS02420 (MRO13_02420) fldB 506464..506982 (-) 519 WP_350940071.1 flavodoxin FldB -
  MRO13_RS02425 (MRO13_02425) xerD 507168..508022 (+) 855 WP_233268213.1 site-specific tyrosine recombinase XerD -
  MRO13_RS02430 (MRO13_02430) - 508038..508790 (+) 753 WP_170906300.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 16274.65 Da        Isoelectric Point: 5.7589

>NTDB_id=676281 MRO13_RS02405 WP_350940067.1 504185..504640(-) (pilA) [Vibrio metschnikovii strain 20CO01TI02]
MKTHYTKQQGFTLIELMIVVAIIGILSAFAVPAYQNYTKKATLAEFPKAMSAIKLAVELCAHENASDAASFATNCVSENN
GVQKEFTLNDIKISASGKDNAVTITAEADKAKGPIAKGEKYLMVAEYKPQGLEWSASCLTADGKEQETYCP

Nucleotide


Download         Length: 456 bp        

>NTDB_id=676281 MRO13_RS02405 WP_350940067.1 504185..504640(-) (pilA) [Vibrio metschnikovii strain 20CO01TI02]
ATGAAAACTCACTACACTAAACAACAAGGCTTTACTCTGATTGAACTGATGATCGTCGTGGCGATCATTGGAATATTATC
AGCTTTTGCTGTACCTGCTTATCAAAACTATACAAAGAAAGCTACACTAGCTGAATTTCCTAAAGCTATGTCAGCTATAA
AGTTAGCCGTTGAATTATGTGCTCACGAAAATGCATCTGATGCAGCAAGTTTTGCAACAAACTGTGTATCTGAAAACAAT
GGGGTTCAAAAAGAATTTACTCTAAACGACATCAAAATATCTGCATCAGGAAAAGATAATGCTGTCACAATTACAGCGGA
AGCAGATAAAGCTAAAGGTCCAATTGCCAAAGGAGAGAAATATTTAATGGTAGCGGAATATAAACCTCAAGGCTTAGAAT
GGTCGGCAAGTTGCTTAACAGCAGATGGTAAAGAACAAGAAACTTACTGCCCATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-30)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

55.844

100

0.57