Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   MUB28_RS08735 Genome accession   NZ_CP094945
Coordinates   1698501..1699442 (-) Length   313 a.a.
NCBI ID   WP_014727726.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain VHProbi R08     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1693501..1704442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUB28_RS08695 (MUB28_08695) - 1693577..1694770 (-) 1194 WP_014608740.1 acetate kinase -
  MUB28_RS08700 (MUB28_08700) comYH 1694826..1695782 (-) 957 WP_011226622.1 class I SAM-dependent methyltransferase Machinery gene
  MUB28_RS08705 (MUB28_08705) comGG 1695827..1696144 (-) 318 WP_002952035.1 competence type IV pilus minor pilin ComGG -
  MUB28_RS08710 (MUB28_08710) comYF 1696122..1696559 (-) 438 WP_014608742.1 competence type IV pilus minor pilin ComGF Machinery gene
  MUB28_RS08715 (MUB28_08715) comGE 1696543..1696836 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  MUB28_RS08720 (MUB28_08720) comYD 1696808..1697236 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  MUB28_RS08725 (MUB28_08725) comYC 1697196..1697522 (-) 327 WP_014727724.1 competence type IV pilus major pilin ComGC Machinery gene
  MUB28_RS08730 (MUB28_08730) comYB 1697519..1698619 (-) 1101 WP_116920398.1 competence type IV pilus assembly protein ComGB Machinery gene
  MUB28_RS08735 (MUB28_08735) comGA/cglA/cilD 1698501..1699442 (-) 942 WP_014727726.1 competence type IV pilus ATPase ComGA Machinery gene
  MUB28_RS08740 (MUB28_08740) - 1699523..1699885 (-) 363 WP_014608746.1 DUF1033 family protein -
  MUB28_RS08745 (MUB28_08745) rpoC 1700044..1703682 (-) 3639 WP_011681690.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35524.70 Da        Isoelectric Point: 6.6091

>NTDB_id=674436 MUB28_RS08735 WP_014727726.1 1698501..1699442(-) (comGA/cglA/cilD) [Streptococcus thermophilus strain VHProbi R08]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVARMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGISYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=674436 MUB28_RS08735 WP_014727726.1 1698501..1699442(-) (comGA/cglA/cilD) [Streptococcus thermophilus strain VHProbi R08]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTCTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGAGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCCGAACGTCGAGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTAATTTCGATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGTGTAACTGTTCTTTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGCGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTTCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.273

99.361

0.649
  comYA Streptococcus mutans UA159

64.537

100

0.645
  comYA Streptococcus mutans UA140

64.537

100

0.645
  comGA/cglA/cilD Streptococcus pneumoniae D39

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae R6

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

63.987

99.361

0.636
  comYA Streptococcus gordonii str. Challis substr. CH1

62.939

100

0.629
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.897

99.681

0.607
  comGA Lactococcus lactis subsp. cremoris KW2

50.804

99.361

0.505
  comGA Latilactobacillus sakei subsp. sakei 23K

38.158

97.125

0.371