Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   lgb_RS00725 Genome accession   NZ_CP094882
Coordinates   147590..148534 (+) Length   314 a.a.
NCBI ID   WP_019293012.1    Uniprot ID   -
Organism   Lactococcus petauri strain B1726     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 142590..153534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  lgb_RS00710 (lgb_00140) - 143851..145452 (-) 1602 WP_019293013.1 GH25 family lysozyme -
  lgb_RS00715 (lgb_00141) guaB 145621..147102 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  lgb_RS00720 (lgb_00142) rpsU 147228..147404 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  lgb_RS00725 (lgb_00143) comGA 147590..148534 (+) 945 WP_019293012.1 competence type IV pilus ATPase ComGA Machinery gene
  lgb_RS00730 (lgb_00144) comGB 148473..149492 (+) 1020 WP_256777882.1 competence type IV pilus assembly protein ComGB Machinery gene
  lgb_RS00735 (lgb_00145) comYC 149511..149816 (+) 306 WP_029345656.1 competence type IV pilus major pilin ComGC Machinery gene
  lgb_RS00740 comGD 149791..150207 (+) 417 WP_019293010.1 competence type IV pilus minor pilin ComGD Machinery gene
  lgb_RS00745 comGE 150239..150472 (+) 234 WP_019293009.1 competence type IV pilus minor pilin ComGE -
  lgb_RS00750 (lgb_00147) comGF 150459..150893 (+) 435 WP_029345654.1 competence type IV pilus minor pilin ComGF -
  lgb_RS00755 (lgb_00148) - 151125..151970 (+) 846 WP_019336001.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  lgb_RS00760 (lgb_00149) - 152041..152925 (-) 885 WP_019293007.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35700.02 Da        Isoelectric Point: 6.9700

>NTDB_id=673917 lgb_RS00725 WP_019293012.1 147590..148534(+) (comGA) [Lactococcus petauri strain B1726]
MIQVLAKNLLQKAVDQMVHDLYLVALEGKYSLYFRTTTERRFEGELTLEQGQALIAHMKFLSGMNLGESRRVQLGACTYI
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFNTIIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDSLIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLSDWELQSSLQRVIYQRLIAGKGLLVCEKEKFEEWKPDEWNDQIDQLVADGFISAVAAAREKIEFSKAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=673917 lgb_RS00725 WP_019293012.1 147590..148534(+) (comGA) [Lactococcus petauri strain B1726]
ATGATACAGGTTTTGGCAAAAAACCTTTTACAAAAAGCAGTAGATCAGATGGTGCACGATCTTTATTTAGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAACAACAGAAAGGAGGTTTGAAGGAGAACTCACCCTTGAGCAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGAGTACAGTTGGGGGCTTGCACTTATATC
TTAGATAAAGGTGAGCAGCGCTTACGTTTATCTACTGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATTCGACTCTT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATCTTTAATACTATTATTGGGGGAAGAGGCCTTTACCTTT
TTTCAGGTCCTGTAGGTTCAGGTAAGACCAGTCTTATGTACAAATTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTAGAAACTGAATTTTTGCAACTTCAAGTCAATAAAGTAATAGGGAATGATTATGA
TTCCTTGATTAAGCTTTCGCTGCGACACAGACCTGATTTACTTATTGTTGGTGAAATTAGGGATAGTCAAACGGCAAAAG
CTGTTCTTCGTGCAAGTTTGACTGGGTATACAGTATTCTCTACTGTTCATGCACGCTCAATATCAGGTGTAATTGCACGC
TTAAAAGAACTTGGATTAAGTGATTGGGAGCTACAATCCAGTCTTCAGCGGGTGATTTATCAACGATTAATTGCAGGAAA
GGGATTGCTTGTTTGTGAAAAGGAAAAGTTTGAAGAATGGAAACCAGACGAATGGAATGACCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

60.191

100

0.602
  comYA Streptococcus gordonii str. Challis substr. CH1

53.651

100

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.716

99.682

0.525
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.564

99.363

0.522
  comYA Streptococcus mutans UA159

50.949

100

0.513
  comYA Streptococcus mutans UA140

50.949

100

0.513