Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MRB47_RS16450 Genome accession   NZ_CP094346
Coordinates   3518702..3519919 (+) Length   405 a.a.
NCBI ID   WP_012655750.1    Uniprot ID   A0AAX1WYX1
Organism   Diaphorobacter sp. LI3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3513702..3524919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRB47_RS16425 (MRB47_16425) rpmA 3514871..3515128 (-) 258 WP_011804286.1 50S ribosomal protein L27 -
  MRB47_RS16430 (MRB47_16430) rplU 3515146..3515457 (-) 312 WP_012655753.1 50S ribosomal protein L21 -
  MRB47_RS16435 (MRB47_16435) - 3515627..3516610 (+) 984 WP_252101223.1 polyprenyl synthetase family protein -
  MRB47_RS16445 (MRB47_16445) pilB 3516948..3518681 (+) 1734 WP_252101224.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MRB47_RS16450 (MRB47_16450) pilC 3518702..3519919 (+) 1218 WP_012655750.1 type II secretion system F family protein Machinery gene
  MRB47_RS16455 (MRB47_16455) - 3519919..3520800 (+) 882 WP_012655749.1 prepilin peptidase -
  MRB47_RS16460 (MRB47_16460) coaE 3520832..3521425 (+) 594 WP_252101225.1 dephospho-CoA kinase -
  MRB47_RS16465 (MRB47_16465) zapD 3521516..3522271 (+) 756 WP_011804250.1 cell division protein ZapD -
  MRB47_RS16470 (MRB47_16470) - 3522278..3522508 (+) 231 WP_252101226.1 DNA gyrase inhibitor YacG -
  MRB47_RS16475 (MRB47_16475) - 3522577..3523023 (-) 447 WP_012655746.1 NUDIX domain-containing protein -
  MRB47_RS16480 (MRB47_16480) - 3523020..3523898 (-) 879 WP_011804247.1 ATP-binding protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44423.44 Da        Isoelectric Point: 9.5091

>NTDB_id=669155 MRB47_RS16450 WP_012655750.1 3518702..3519919(+) (pilC) [Diaphorobacter sp. LI3]
MATVASRGIKDFVFEWEGKDRSGKIVRGEVRAAGENQVKATLRRQGVLPTKIKKRRMSAGKKIKPKDIALFTRQMATMMK
AGVPLLQAFDIVGRGNTNPSVTKLLNDIRGDVETGTSLNAAFRKYPMYFDSLYCNLVEAGEAAGILEALLDRLALYMEKT
EAIKSKIRSALMYPISVIIVAFVVVTIIMIFVIPAFKEVFTSFGADLPAPTLFVMAISEFFVAYWWLIFGVIGGGFYFFM
QAWKRSEKMQRFMDRFLLKMPIFGALIDKSCVARWTRTLSTMFAAGVPLVEALDSVAGASGNSVYAIATEKIQQEVSTGT
SLTAAMGNANVFPSMVLQMCAIGEESGSIDHMLGKAADFYEAEVDEMVAGLSSLMEPIIIVFLGTLIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=669155 MRB47_RS16450 WP_012655750.1 3518702..3519919(+) (pilC) [Diaphorobacter sp. LI3]
ATGGCGACCGTTGCATCGAGGGGCATCAAGGACTTTGTCTTCGAGTGGGAGGGCAAGGATCGTAGCGGCAAGATCGTGCG
CGGCGAAGTACGCGCTGCGGGAGAGAACCAGGTCAAGGCGACGCTGCGGCGGCAGGGCGTTCTGCCAACCAAGATCAAGA
AGCGCCGGATGAGCGCGGGCAAAAAGATCAAGCCCAAAGACATCGCGCTGTTCACGCGCCAGATGGCCACGATGATGAAG
GCCGGTGTTCCACTGCTTCAGGCATTTGATATCGTGGGCCGGGGTAATACCAATCCCAGTGTGACCAAGCTGCTCAACGA
TATCCGCGGAGATGTGGAGACAGGAACGTCCCTGAATGCCGCATTCCGTAAATATCCGATGTACTTCGACAGCCTTTACT
GCAACCTCGTAGAGGCCGGCGAAGCCGCCGGTATTCTGGAAGCGTTGCTCGACCGGCTGGCCCTGTACATGGAGAAGACC
GAGGCCATCAAATCCAAGATCCGGTCGGCGCTGATGTATCCGATCTCGGTGATCATCGTTGCGTTTGTCGTGGTGACGAT
CATCATGATTTTCGTGATTCCCGCGTTCAAAGAGGTTTTCACTTCCTTCGGCGCCGACCTGCCCGCTCCTACCCTGTTCG
TGATGGCCATCAGCGAGTTCTTCGTGGCTTACTGGTGGCTGATATTCGGCGTCATCGGCGGGGGGTTCTACTTCTTCATG
CAGGCCTGGAAGCGCAGCGAAAAAATGCAGCGTTTCATGGACCGTTTCCTGTTGAAAATGCCCATCTTCGGTGCATTGAT
CGACAAGTCCTGCGTTGCCCGTTGGACCCGCACCCTGTCGACCATGTTCGCTGCCGGTGTGCCATTGGTGGAGGCACTGG
ACTCGGTGGCCGGTGCATCGGGCAACTCGGTCTACGCCATCGCGACGGAGAAGATTCAGCAGGAAGTCTCCACAGGTACC
AGCCTGACGGCCGCCATGGGGAATGCCAACGTATTCCCCTCCATGGTGCTGCAGATGTGTGCGATCGGCGAGGAATCCGG
CTCCATCGACCACATGCTGGGTAAGGCCGCCGACTTCTATGAAGCCGAAGTGGATGAAATGGTGGCCGGCCTGTCCAGCC
TGATGGAGCCCATCATCATCGTGTTCCTGGGCACGCTGATCGGTGGCATCGTGGTGTCGATGTACCTTCCGATCTTCAAG
CTCGGGCAAGTGGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.309

100

0.553
  pilG Neisseria gonorrhoeae MS11

53.535

97.778

0.523
  pilG Neisseria meningitidis 44/76-A

53.283

97.778

0.521
  pilC Acinetobacter baylyi ADP1

50.754

98.272

0.499
  pilC Legionella pneumophila strain ERS1305867

49.749

98.272

0.489
  pilC Acinetobacter baumannii D1279779

49.874

98.025

0.489
  pilC Vibrio cholerae strain A1552

40.404

97.778

0.395
  pilC Vibrio campbellii strain DS40M4

39.9

99.012

0.395
  pilC Thermus thermophilus HB27

37.845

98.519

0.373

Multiple sequence alignment