Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MRB47_RS16445 Genome accession   NZ_CP094346
Coordinates   3516948..3518681 (+) Length   577 a.a.
NCBI ID   WP_252101224.1    Uniprot ID   -
Organism   Diaphorobacter sp. LI3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3511948..3523681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRB47_RS16415 (MRB47_16415) proB 3512435..3513577 (-) 1143 WP_011804288.1 glutamate 5-kinase -
  MRB47_RS16420 (MRB47_16420) cgtA 3513651..3514724 (-) 1074 WP_012655754.1 Obg family GTPase CgtA -
  MRB47_RS16425 (MRB47_16425) rpmA 3514871..3515128 (-) 258 WP_011804286.1 50S ribosomal protein L27 -
  MRB47_RS16430 (MRB47_16430) rplU 3515146..3515457 (-) 312 WP_012655753.1 50S ribosomal protein L21 -
  MRB47_RS16435 (MRB47_16435) - 3515627..3516610 (+) 984 WP_252101223.1 polyprenyl synthetase family protein -
  MRB47_RS16445 (MRB47_16445) pilB 3516948..3518681 (+) 1734 WP_252101224.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MRB47_RS16450 (MRB47_16450) pilC 3518702..3519919 (+) 1218 WP_012655750.1 type II secretion system F family protein Machinery gene
  MRB47_RS16455 (MRB47_16455) - 3519919..3520800 (+) 882 WP_012655749.1 prepilin peptidase -
  MRB47_RS16460 (MRB47_16460) coaE 3520832..3521425 (+) 594 WP_252101225.1 dephospho-CoA kinase -
  MRB47_RS16465 (MRB47_16465) zapD 3521516..3522271 (+) 756 WP_011804250.1 cell division protein ZapD -
  MRB47_RS16470 (MRB47_16470) - 3522278..3522508 (+) 231 WP_252101226.1 DNA gyrase inhibitor YacG -
  MRB47_RS16475 (MRB47_16475) - 3522577..3523023 (-) 447 WP_012655746.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63649.01 Da        Isoelectric Point: 5.3559

>NTDB_id=669154 MRB47_RS16445 WP_252101224.1 3516948..3518681(+) (pilB) [Diaphorobacter sp. LI3]
MAAIDTQKEAQPIALPGLARALMSVGKLDQKAAEEIYKKSQNSRSSFIAELTESKIITPSDLAHTVSTVFGAPLLDVDAI
DRQRIPKDLLDVKLCQSYKVIALSKRNNRLIVATADPTFQEAAEKIKFTTQMGVDWIITEYDKLLRLIEASTKSGTETLD
GINSSNDFEFGDIPVDEAPDEDADNPAEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHHYRVRFRIDGELREIASPPI
AIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
LLQAINRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDASTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPNCKAPVDIPH
ETLLEAGYRDEEIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPVSEEIQRIILRDGSALEIAEQARLEGVRSLRESGLHK
AKMGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=669154 MRB47_RS16445 WP_252101224.1 3516948..3518681(+) (pilB) [Diaphorobacter sp. LI3]
ATGGCTGCTATCGACACACAGAAAGAAGCTCAACCCATCGCCTTACCCGGACTGGCGCGCGCGTTGATGTCTGTGGGCAA
ATTGGATCAAAAAGCGGCGGAAGAGATTTACAAAAAGTCACAAAATAGCCGCAGTAGCTTTATTGCAGAGCTCACGGAAT
CGAAAATCATCACGCCAAGTGATCTGGCCCATACGGTATCAACCGTATTTGGTGCCCCCTTGCTAGATGTCGATGCGATC
GACCGGCAGCGCATCCCCAAAGACCTGTTGGACGTCAAACTGTGCCAGTCCTACAAAGTCATCGCTCTCAGTAAGCGCAA
TAACCGGCTCATCGTCGCCACCGCGGATCCGACATTTCAAGAGGCCGCAGAAAAGATCAAGTTCACCACACAAATGGGGG
TGGACTGGATCATCACGGAATATGACAAGCTGCTCCGCTTGATTGAGGCGAGCACGAAGAGCGGTACAGAAACGCTCGAC
GGCATTAACAGTAGTAATGATTTTGAGTTCGGCGACATTCCCGTTGATGAAGCTCCGGACGAGGATGCCGACAATCCCGC
GGAAGTCGAGGATGCTCCCGTTGTCAAATTTCTGCACAAGATGCTGTTGGATGCTTTCAATATGCGTGCATCCGATCTGC
ATTTTGAGCCCTACGAGCACCATTACCGTGTCCGCTTCCGCATAGATGGCGAATTGCGCGAAATCGCATCGCCGCCTATT
GCCATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCCCGCCTGGATATTTCGGAAAAACGCGTGCCCCAGGATGG
CCGCATGAAGCTCAAGGTTGGGCCGGACCGGGTTATCGATTTCCGCGTCAGCACCCTGCCTACGCTCTTTGGCGAGAAGA
TCGTGATCCGTATTCTCGATCCGAGCAGCGCCAAGCTGGGTATTGACGCACTGGGATATGAGCCCGAGGAAAAGGAGCGC
TTGCTGCAGGCCATCAACCGCCCTTATGGAATGATCTTAGTGACCGGGCCCACGGGCTCCGGGAAGACCGTATCGCTCTA
CACATGTCTCAATCTGTTGAACAAGCCCGGCGTCAATATTGCGACCGCTGAGGACCCTTCGGAAATCAACCTCCCCGGTG
TAAACCAAGTCAACGTCAACGAGAAAGCCGGCCTCACGTTTGCCGTCGCGCTTAAGTCATTCCTTCGCCAAGATCCGGAC
ATCATCATGGTGGGTGAAATCCGGGATCTGGAAACGGCAGACATTTCAATCAAAGCCGCGCAGACGGGTCACTTGGTGCT
CTCTACCCTGCACACCAATGACGCTTCCACGACATTGACGCGCATGCGCAATATGGGCATCGCGCCCTTCAATATCGCAT
CCAGCGTGATTCTTATTACGGCGCAGCGACTGGCGCGTCGCTTGTGCCCCAATTGCAAGGCGCCCGTGGACATTCCGCAT
GAGACGCTGCTGGAGGCCGGCTACCGGGACGAGGAGATCGACGGCTCATGGGTTACCTACCGACCGGTGGGCTGCTCCGC
CTGCAATAACGGATACAAGGGGCGCGTGGGCATTTACCAGGTCATGCCGGTGTCCGAAGAAATCCAGCGCATCATCCTGC
GCGATGGAAGCGCACTGGAAATTGCCGAACAGGCACGCCTGGAAGGCGTCCGTTCTCTGCGTGAATCCGGGCTTCACAAG
GCAAAAATGGGGTTGACCTCGTTGGAAGAGGTCTTGGCTGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.181

99.48

0.539
  pilB Acinetobacter baumannii D1279779

53.546

97.747

0.523
  pilF Neisseria gonorrhoeae MS11

52.212

97.92

0.511
  pilB Legionella pneumophila strain ERS1305867

49.912

97.92

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

45.536

97.054

0.442
  pilB Vibrio campbellii strain DS40M4

45.276

97.227

0.44
  pilB Vibrio cholerae strain A1552

46.692

91.681

0.428
  pilF Thermus thermophilus HB27

39.726

100

0.402
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.449

92.548

0.374

Multiple sequence alignment