Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MRP56_RS03170 Genome accession   NZ_CP094267
Coordinates   665718..666827 (-) Length   369 a.a.
NCBI ID   WP_017411211.1    Uniprot ID   A0A1C2L453
Organism   Aeromonas hydrophila strain A008N2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 660718..671827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRP56_RS03150 brnQ 661176..662483 (-) 1308 WP_073352588.1 branched-chain amino acid transport system II carrier protein -
  MRP56_RS03155 - 662674..663384 (-) 711 WP_073351735.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  MRP56_RS03160 srmB 663513..664736 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  MRP56_RS03165 yaaA 664913..665686 (-) 774 WP_073351733.1 peroxide stress protein YaaA -
  MRP56_RS03170 pilU 665718..666827 (-) 1110 WP_017411211.1 type IVa pilus ATPase TapU Machinery gene
  MRP56_RS03175 pilT 666856..667890 (-) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  MRP56_RS03180 - 667930..668631 (+) 702 WP_073351730.1 YggS family pyridoxal phosphate-dependent enzyme -
  MRP56_RS03185 proC 668690..669514 (+) 825 WP_073351728.1 pyrroline-5-carboxylate reductase -
  MRP56_RS03190 - 669555..670106 (+) 552 WP_016351969.1 YggT family protein -
  MRP56_RS03195 yggU 670106..670405 (+) 300 WP_011707385.1 DUF167 family protein YggU -
  MRP56_RS03200 - 670424..670843 (+) 420 WP_011707384.1 DUF4426 domain-containing protein -
  MRP56_RS03205 - 670900..671211 (-) 312 WP_042064670.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41035.17 Da        Isoelectric Point: 6.5035

>NTDB_id=668485 MRP56_RS03170 WP_017411211.1 665718..666827(-) (pilU) [Aeromonas hydrophila strain A008N2]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=668485 MRP56_RS03170 WP_017411211.1 665718..666827(-) (pilU) [Aeromonas hydrophila strain A008N2]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGTAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCATCTGCAGTCGCTGGCAGAAGCCCCCCTCGACAAGGCCAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAGCGCTACCTGCACACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGTATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAATTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTAGGCATAGACACAGCGTCATTCGACGT
GGCGCTGAAGAGTTCGCTGCGCCAGGCGCCAGATGTGATCCTGATCGGCGAAATTCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGACGCCGTCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCG
TCCGCAAAGGGGAGATGCATCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C2L453

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36

Multiple sequence alignment