Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   MPM64_RS01500 Genome accession   NZ_CP093957
Coordinates   328031..328489 (-) Length   152 a.a.
NCBI ID   WP_002365911.1    Uniprot ID   Q8VT46
Organism   Enterococcus faecalis strain NY13321     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 276158..329779 328031..328489 within 0


Gene organization within MGE regions


Location: 276158..329779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPM64_RS15870 - 276446..276574 (+) 129 WP_002361208.1 hypothetical protein -
  MPM64_RS01250 (MPM64_01250) - 277841..278383 (-) 543 WP_002392646.1 ABC transporter ATP-binding protein -
  MPM64_RS01255 (MPM64_01255) - 278408..280315 (-) 1908 WP_002392645.1 FtsX-like permease family protein -
  MPM64_RS01260 (MPM64_01260) - 281107..281268 (-) 162 WP_002366123.1 hypothetical protein -
  MPM64_RS01265 (MPM64_01265) - 281487..282665 (+) 1179 WP_002328152.1 IS256-like element ISEfm2 family transposase -
  MPM64_RS01270 (MPM64_01270) xylB 282946..284436 (-) 1491 WP_002363087.1 xylulokinase -
  MPM64_RS01275 (MPM64_01275) xylA 284498..285805 (-) 1308 WP_002358555.1 xylose isomerase -
  MPM64_RS01280 (MPM64_01280) - 285910..286317 (-) 408 WP_002358554.1 PTS sugar transporter subunit IIA -
  MPM64_RS01285 (MPM64_01285) - 286329..286811 (-) 483 WP_002358553.1 PTS sugar transporter subunit IIB -
  MPM64_RS01290 (MPM64_01290) - 286836..287648 (-) 813 WP_002358552.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
  MPM64_RS01295 (MPM64_01295) - 287641..288426 (-) 786 WP_002358551.1 PTS sugar transporter subunit IIC -
  MPM64_RS01300 (MPM64_01300) - 288426..290501 (-) 2076 WP_002382850.1 TIM-barrel domain-containing protein -
  MPM64_RS01305 (MPM64_01305) - 290859..292025 (+) 1167 WP_002358549.1 ROK family transcriptional regulator -
  MPM64_RS15875 - 292266..292397 (+) 132 WP_002358548.1 hypothetical protein -
  MPM64_RS01310 (MPM64_01310) - 292533..292808 (+) 276 WP_002367939.1 transposase -
  MPM64_RS01315 (MPM64_01315) - 292911..293225 (+) 315 Protein_266 DDE-type integrase/transposase/recombinase -
  MPM64_RS15940 - 293762..294093 (+) 332 Protein_267 PTS glucose transporter subunit IIA -
  MPM64_RS01320 (MPM64_01320) - 294294..295427 (-) 1134 WP_002377959.1 NAD(P)/FAD-dependent oxidoreductase -
  MPM64_RS01325 (MPM64_01325) - 295530..296600 (-) 1071 WP_010710139.1 ammonium transporter -
  MPM64_RS01330 (MPM64_01330) - 296794..297197 (-) 404 Protein_270 NusG domain II-containing protein -
  MPM64_RS01335 (MPM64_01335) - 297249..297626 (-) 378 WP_002382844.1 hypothetical protein -
  MPM64_RS01340 (MPM64_01340) rpmF 297648..297821 (-) 174 WP_002358539.1 50S ribosomal protein L32 -
  MPM64_RS01345 (MPM64_01345) - 298168..299346 (-) 1179 WP_002328152.1 IS256-like element ISEfm2 family transposase -
  MPM64_RS01350 (MPM64_01350) - 299493..299732 (+) 240 Protein_274 YibE/F family protein -
  MPM64_RS01355 (MPM64_01355) - 299836..301008 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  MPM64_RS01360 (MPM64_01360) aph(2'')-Ia 301138..302577 (-) 1440 WP_001028144.1 aminoglycoside O-phosphotransferase APH(2'')-Ia -
  MPM64_RS01365 (MPM64_01365) - 302578..302970 (-) 393 WP_000393259.1 GNAT family N-acetyltransferase -
  MPM64_RS01370 (MPM64_01370) - 303027..304199 (-) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  MPM64_RS01375 (MPM64_01375) - 304296..305108 (-) 813 WP_226013816.1 cysteine peptidase family C39 domain-containing protein -
  MPM64_RS01380 (MPM64_01380) - 305120..308101 (-) 2982 WP_002370929.1 type 2 lanthipeptide synthetase LanM family protein -
  MPM64_RS01385 (MPM64_01385) cylL-S 308162..308353 (-) 192 WP_002358181.1 lanthipeptide cytolysin subunit CylL-S -
  MPM64_RS01390 (MPM64_01390) cylL-L 308387..308593 (-) 207 WP_002358485.1 lanthipeptide cytolysin subunit CylL-L -
  MPM64_RS01395 (MPM64_01395) cylR2 308997..309197 (+) 201 WP_002370931.1 cytolysin regulator CylR2 -
  MPM64_RS01400 (MPM64_01400) - 309535..309849 (-) 315 WP_002403043.1 hypothetical protein -
  MPM64_RS01405 (MPM64_01405) - 310022..310666 (-) 645 WP_002377952.1 IS6 family transposase -
  MPM64_RS01410 (MPM64_01410) bsh 310890..311864 (+) 975 WP_002355428.1 choloylglycine hydrolase -
  MPM64_RS01415 (MPM64_01415) - 312533..313141 (-) 609 Protein_287 IS6 family transposase -
  MPM64_RS01420 (MPM64_01420) - 313173..313358 (-) 186 WP_002358660.1 hypothetical protein -
  MPM64_RS01425 (MPM64_01425) - 313426..314320 (-) 895 Protein_289 IS256 family transposase -
  MPM64_RS01430 (MPM64_01430) - 314441..315085 (+) 645 WP_002377952.1 IS6 family transposase -
  MPM64_RS01435 (MPM64_01435) - 315276..315551 (-) 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin -
  MPM64_RS01440 (MPM64_01440) - 315544..315810 (-) 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
  MPM64_RS01445 (MPM64_01445) - 315921..316505 (-) 585 WP_226013793.1 thermonuclease family protein -
  MPM64_RS01450 (MPM64_01450) - 316529..316945 (-) 417 WP_010710134.1 single-stranded DNA-binding protein -
  MPM64_RS01455 (MPM64_01455) - 317021..317182 (-) 162 WP_226013796.1 DUF3850 domain-containing protein -
  MPM64_RS01460 (MPM64_01460) - 317283..317783 (-) 501 WP_002360775.1 DnaJ domain-containing protein -
  MPM64_RS01465 (MPM64_01465) - 317816..318082 (-) 267 WP_002377947.1 hypothetical protein -
  MPM64_RS01470 (MPM64_01470) - 318674..319162 (-) 489 WP_010710133.1 hypothetical protein -
  MPM64_RS01475 (MPM64_01475) - 319168..319953 (-) 786 WP_002360778.1 replication-relaxation family protein -
  MPM64_RS01480 (MPM64_01480) - 320476..322734 (-) 2259 WP_086321360.1 TraM recognition domain-containing protein -
  MPM64_RS01485 (MPM64_01485) - 322721..325066 (-) 2346 WP_142431121.1 hypothetical protein -
  MPM64_RS01490 (MPM64_01490) - 325079..325477 (-) 399 WP_002370287.1 DUF4923 family protein -
  MPM64_RS01495 (MPM64_01495) - 325434..327926 (-) 2493 WP_002377939.1 ATP-binding protein -
  MPM64_RS01500 (MPM64_01500) ssb 328031..328489 (-) 459 WP_002365911.1 single-stranded DNA-binding protein Machinery gene
  MPM64_RS01505 (MPM64_01505) - 328584..329066 (-) 483 WP_002377938.1 hypothetical protein -
  MPM64_RS01510 (MPM64_01510) - 329098..329487 (-) 390 WP_002360791.1 TcpE family conjugal transfer membrane protein -
  MPM64_RS01515 (MPM64_01515) - 329487..329762 (-) 276 WP_002360793.1 hypothetical protein -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 17179.08 Da        Isoelectric Point: 4.6511

>NTDB_id=665886 MPM64_RS01500 WP_002365911.1 328031..328489(-) (ssb) [Enterococcus faecalis strain NY13321]
MINNVTLVGRLTKDPDLRYTQSGTAVGQFTLAINRNFTNANNEREADFINCVIWRKAAESLANYATKGTLIGLTGRIQTR
NYENQQGQRIYVTEVVTESFQLLESREVNEQRKEQATGKATFDKQSMDKPDPLDPFSPENSIVDISDNDLPF

Nucleotide


Download         Length: 459 bp        

>NTDB_id=665886 MPM64_RS01500 WP_002365911.1 328031..328489(-) (ssb) [Enterococcus faecalis strain NY13321]
TTGATTAATAACGTTACATTAGTTGGACGATTAACCAAAGACCCAGATTTAAGGTATACGCAAAGTGGAACAGCCGTAGG
TCAATTTACGTTGGCCATTAATCGCAACTTTACCAATGCTAACAATGAAAGGGAAGCAGATTTTATCAACTGTGTTATTT
GGCGGAAAGCTGCAGAGTCATTAGCAAATTATGCAACAAAAGGGACTCTGATCGGTTTAACTGGTCGCATTCAAACAAGA
AACTATGAGAATCAACAAGGCCAGCGTATTTATGTAACTGAGGTTGTCACAGAAAGCTTCCAACTATTAGAATCAAGAGA
AGTAAACGAGCAACGAAAAGAACAGGCTACAGGTAAAGCTACGTTTGATAAACAGTCAATGGATAAACCTGATCCTCTGG
ATCCATTTTCGCCAGAAAATAGCATAGTGGATATTTCTGATAATGACCTGCCGTTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VT46

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

56.471

100

0.632
  ssbA Bacillus subtilis subsp. subtilis str. 168

47.283

100

0.572
  ssbB Bacillus subtilis subsp. subtilis str. 168

56.604

69.737

0.395
  ssbB/cilA Streptococcus mitis NCTC 12261

50

76.316

0.382
  ssbB Streptococcus sobrinus strain NIDR 6715-7

53.774

69.737

0.375
  ssbB/cilA Streptococcus pneumoniae TIGR4

49.138

76.316

0.375
  ssbB/cilA Streptococcus pneumoniae Rx1

48.276

76.316

0.368
  ssbB/cilA Streptococcus pneumoniae D39

48.276

76.316

0.368
  ssbB/cilA Streptococcus pneumoniae R6

48.276

76.316

0.368
  ssbB/cilA Streptococcus mitis SK321

48.276

76.316

0.368