Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MN178_RS25995 Genome accession   NZ_CP093356
Coordinates   5533348..5534568 (+) Length   406 a.a.
NCBI ID   WP_016253888.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain E125     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5528348..5539568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN178_RS25975 (MN178_25975) nadC 5529927..5530775 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MN178_RS25985 (MN178_25985) pilA 5530963..5531415 (-) 453 WP_024947914.1 pilin Machinery gene
  MN178_RS25990 (MN178_25990) pilB 5531644..5533344 (+) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MN178_RS25995 (MN178_25995) pilC 5533348..5534568 (+) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  MN178_RS26000 (MN178_26000) pilD 5534572..5535444 (+) 873 WP_023087804.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  MN178_RS26005 (MN178_26005) coaE 5535441..5536052 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  MN178_RS26010 (MN178_26010) yacG 5536049..5536249 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  MN178_RS26015 (MN178_26015) - 5536286..5536495 (-) 210 WP_003094660.1 hypothetical protein -
  MN178_RS26020 (MN178_26020) - 5536601..5537290 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  MN178_RS26025 (MN178_26025) - 5537287..5537757 (-) 471 WP_003103866.1 hypothetical protein -
  MN178_RS26030 (MN178_26030) - 5537754..5538179 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  MN178_RS26035 (MN178_26035) - 5538312..5538941 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  MN178_RS26040 (MN178_26040) - 5538938..5539387 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44549.37 Da        Isoelectric Point: 9.6983

>NTDB_id=663932 MN178_RS25995 WP_016253888.1 5533348..5534568(+) (pilC) [Pseudomonas aeruginosa strain E125]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFEGFGAELPAFTQMIVNLSEFMQEWWFFIILAIAIFGFAFK
ELHKRSQKFRDTLDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=663932 MN178_RS25995 WP_016253888.1 5533348..5534568(+) (pilC) [Pseudomonas aeruginosa strain E125]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAAGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTGAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAGGGCTTCGACAACCCCAACATGCGCAAGCTTGTGGATGA
AATCAAACAGGAAGTTTCCTCAGGTAACAGCCTAGCCAACTCCTTGAGAAAAAAGCCCCAGTATTTTGACGAGCTTTATT
GCAACCTGGTAGATGCAGGGGAACAGTCTGGCGCCTTGGAAAACCTTCTCGATCGGGTGGCAACCTATAAAGAAAAGACG
GAATCACTGAAAGCCAAGATCAAAAAGGCGATGACCTATCCCATTGCCGTCATCATTGTCGCACTGATTGTATCTGCGAT
CCTCCTGATTAAAGTGGTTCCACAATTTCAGTCGGTCTTTGAAGGTTTCGGCGCGGAACTTCCCGCCTTTACCCAGATGA
TTGTCAATCTATCGGAGTTCATGCAGGAGTGGTGGTTCTTCATCATACTGGCGATAGCGATATTTGGCTTTGCATTCAAA
GAATTGCATAAACGCTCACAAAAATTCCGTGACACACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCTGCGGTCGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCGGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

76.543

99.754

0.764
  pilC Acinetobacter baumannii D1279779

61.386

99.507

0.611
  pilC Acinetobacter baylyi ADP1

60.837

100

0.608
  pilC Legionella pneumophila strain ERS1305867

55.051

97.537

0.537
  pilG Neisseria gonorrhoeae MS11

46.173

99.754

0.461
  pilG Neisseria meningitidis 44/76-A

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

42.065

97.783

0.411
  pilC Thermus thermophilus HB27

36.908

98.768

0.365