Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MN178_RS25990 Genome accession   NZ_CP093356
Coordinates   5531644..5533344 (+) Length   566 a.a.
NCBI ID   WP_009875880.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain E125     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5526644..5538344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN178_RS25965 (MN178_25965) ampD 5526669..5527235 (-) 567 WP_023084738.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MN178_RS25970 (MN178_25970) - 5527386..5529641 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  MN178_RS25975 (MN178_25975) nadC 5529927..5530775 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MN178_RS25985 (MN178_25985) pilA 5530963..5531415 (-) 453 WP_024947914.1 pilin Machinery gene
  MN178_RS25990 (MN178_25990) pilB 5531644..5533344 (+) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MN178_RS25995 (MN178_25995) pilC 5533348..5534568 (+) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  MN178_RS26000 (MN178_26000) pilD 5534572..5535444 (+) 873 WP_023087804.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  MN178_RS26005 (MN178_26005) coaE 5535441..5536052 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  MN178_RS26010 (MN178_26010) yacG 5536049..5536249 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  MN178_RS26015 (MN178_26015) - 5536286..5536495 (-) 210 WP_003094660.1 hypothetical protein -
  MN178_RS26020 (MN178_26020) - 5536601..5537290 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  MN178_RS26025 (MN178_26025) - 5537287..5537757 (-) 471 WP_003103866.1 hypothetical protein -
  MN178_RS26030 (MN178_26030) - 5537754..5538179 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62383.27 Da        Isoelectric Point: 5.5590

>NTDB_id=663931 MN178_RS25990 WP_009875880.1 5531644..5533344(+) (pilB) [Pseudomonas aeruginosa strain E125]
MNDSIQLSGLSRQLVQANLLDEKTALQAQTQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDRESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDAANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFENATDGLAGLDDVDLE
GLDVGVKETSGQEDTGAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=663931 MN178_RS25990 WP_009875880.1 5531644..5533344(+) (pilB) [Pseudomonas aeruginosa strain E125]
ATGAACGACAGCATCCAACTGAGCGGTCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCCTGCA
GGCCCAGACGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGACAGGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAGCACCGGGTCATTCCCCTGTGGCGACGGGGCAACAAGTTGTTCGTAGGCATCTC
CGACGCCGCCAACCACCAGGCCATCAACGACGTCCAGTTCAGCACTGGCCTGACGACCGAGGCCATTCTGGTCGAGGACG
ACAAGCTCGGCCTTGCCATCGACAAGCTATTCGAGAATGCAACCGACGGCTTGGCAGGTCTCGATGACGTGGACCTGGAA
GGACTGGATGTAGGCGTCAAGGAAACGTCCGGACAAGAAGATACTGGCGCGGAGGCAGACGACGCTCCCGTCGTCCGCTT
CGTCAACAAGATGCTGCTGGACGCTATCAAAGGCGGGTCTTCCGACTTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTTCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTGGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTGTACACCGGTCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTACCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAGCACGACGTACCGAAGGAAACCCTGCTTCACGAAGGTTTCCCAGAA
GAGTTGATCGGCACCTTCAAGCTGTATTCACCAGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.719

100

0.581
  pilB Acinetobacter baylyi ADP1

57.371

99.47

0.571
  pilB Legionella pneumophila strain ERS1305867

54.77

100

0.548
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

50.444

99.47

0.502
  pilB Vibrio cholerae strain A1552

50

100

0.5
  pilB Vibrio campbellii strain DS40M4

49.113

99.647

0.489
  pilF Thermus thermophilus HB27

39.855

97.527

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.417

93.11

0.376