Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MNO08_RS03880 Genome accession   NZ_CP093333
Coordinates   863997..865205 (-) Length   402 a.a.
NCBI ID   WP_047541163.1    Uniprot ID   -
Organism   Pseudomonas simiae strain H4-B59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 858997..870205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNO08_RS03840 (MNO08_03840) - 859915..860088 (-) 174 WP_010213243.1 DUF3094 family protein -
  MNO08_RS03845 (MNO08_03845) - 860143..860772 (-) 630 WP_010213242.1 DUF1780 domain-containing protein -
  MNO08_RS03850 (MNO08_03850) - 860876..861343 (+) 468 WP_010213240.1 hypothetical protein -
  MNO08_RS03855 (MNO08_03855) - 861340..862029 (+) 690 WP_010213238.1 energy-coupling factor ABC transporter permease -
  MNO08_RS03860 (MNO08_03860) - 862105..862320 (+) 216 WP_003171680.1 hypothetical protein -
  MNO08_RS03865 (MNO08_03865) yacG 862317..862517 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  MNO08_RS03870 (MNO08_03870) coaE 862514..863137 (-) 624 WP_010213235.1 dephospho-CoA kinase -
  MNO08_RS03875 (MNO08_03875) pilD 863134..864000 (-) 867 WP_047541162.1 A24 family peptidase Machinery gene
  MNO08_RS03880 (MNO08_03880) pilC 863997..865205 (-) 1209 WP_047541163.1 type II secretion system F family protein Machinery gene
  MNO08_RS03885 (MNO08_03885) - 865399..865821 (+) 423 WP_187517330.1 pilin -
  MNO08_RS03890 (MNO08_03890) - 865857..867257 (+) 1401 WP_187517331.1 O-antigen ligase family protein -
  MNO08_RS03900 (MNO08_03900) - 867524..867829 (+) 306 WP_010213230.1 DUF6388 family protein -
  MNO08_RS03905 (MNO08_03905) nadC 867877..868725 (-) 849 WP_010213228.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43476.92 Da        Isoelectric Point: 10.1005

>NTDB_id=663710 MNO08_RS03880 WP_047541163.1 863997..865205(-) (pilC) [Pseudomonas simiae strain H4-B59]
MDNASTLYAWEGINRKGRRVSGQTTGHNPALVKAQLRKQGICPGRVRKKSPVLSSLAPSITSTDITLFTRQLATLLKAGI
PLLQAFDIIAEGFDNRALRERVQGLKQAIEAGTSLAEALRKQPRCFDELYCNLVAAGEQAGALETLLERVAIHREKSEQL
KTRIKKAMTYPITVLVVASLVTGVLLVHVVPQFQNLFAGVDGKLPGFTLQVIGLSEFMQKAWWAVALGFLAGGLGLREAY
RRLADFRLWLDSCLLKAPVAGKLLSKSAVARFARTLSTTFAAGVPLLQALDSVAGAAGSGPFRRAIEQMRRDVATGMPLN
QSMLTSGLFPGMAIQMTAIGEESGTLDRMLEKVANHYEADVDNLVDHLTHLMEPLIMVVLGGIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=663710 MNO08_RS03880 WP_047541163.1 863997..865205(-) (pilC) [Pseudomonas simiae strain H4-B59]
ATGGACAACGCTTCAACGCTCTACGCCTGGGAAGGCATCAACCGCAAAGGACGCAGGGTGTCCGGGCAAACCACCGGACA
CAACCCTGCCCTGGTGAAGGCACAATTGCGAAAACAGGGCATCTGCCCGGGGCGTGTGCGCAAAAAATCCCCAGTGCTGT
CGAGCCTGGCACCCTCGATCACATCAACCGACATCACCCTGTTCACTCGCCAACTGGCCACCCTGCTGAAGGCCGGTATT
CCGCTGCTGCAGGCCTTCGACATCATCGCTGAGGGCTTCGACAACCGCGCCTTGCGTGAGCGTGTGCAAGGGTTGAAACA
AGCCATCGAAGCCGGCACCAGCCTTGCCGAGGCGCTGCGCAAGCAGCCGCGTTGTTTCGACGAGCTGTACTGCAACCTGG
TGGCCGCCGGCGAACAGGCCGGAGCACTTGAAACCTTGCTGGAGCGCGTGGCGATCCACCGGGAAAAGAGCGAGCAGCTA
AAGACGAGGATCAAAAAAGCCATGACCTATCCGATCACCGTGCTGGTGGTCGCCAGCCTCGTCACCGGCGTGCTGCTGGT
CCACGTCGTGCCACAGTTCCAGAACCTGTTTGCCGGGGTCGACGGCAAGCTGCCGGGTTTCACCCTGCAGGTCATCGGCC
TTTCCGAGTTCATGCAGAAAGCCTGGTGGGCCGTGGCGCTGGGCTTTCTTGCGGGAGGCCTGGGGCTGCGCGAAGCGTAT
CGGCGCTTGGCGGATTTCCGTCTATGGCTGGACAGCTGTTTGTTGAAAGCGCCCGTGGCAGGCAAACTGCTGAGCAAATC
CGCCGTGGCCCGCTTCGCCCGCACACTGTCCACCACGTTTGCCGCCGGCGTTCCGTTACTGCAGGCACTGGATTCAGTGG
CCGGTGCCGCCGGTTCCGGGCCATTCAGACGGGCAATCGAACAGATGCGACGCGATGTAGCGACTGGCATGCCATTGAAC
CAATCCATGCTCACCAGCGGCCTGTTCCCCGGCATGGCGATCCAGATGACAGCCATCGGCGAAGAGTCGGGCACACTGGA
CCGCATGCTGGAAAAGGTCGCCAACCATTACGAGGCCGATGTGGACAACCTGGTCGATCACCTCACCCACCTCATGGAGC
CCCTGATCATGGTGGTCCTGGGAGGCATTGTCGGCGCATTGGTGATCGCCATGTACCTGCCAGTCTTCCAGTTGGGGAGC
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.848

98.507

0.59
  pilC Acinetobacter baumannii D1279779

53.418

98.259

0.525
  pilC Acinetobacter baylyi ADP1

51.768

98.507

0.51
  pilC Legionella pneumophila strain ERS1305867

50.253

98.507

0.495
  pilC Vibrio cholerae strain A1552

41.414

98.507

0.408
  pilG Neisseria gonorrhoeae MS11

41.927

95.522

0.4
  pilC Vibrio campbellii strain DS40M4

40.355

98.01

0.396
  pilG Neisseria meningitidis 44/76-A

41.146

95.522

0.393
  pilC Thermus thermophilus HB27

36.341

99.254

0.361