Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MN210_RS00800 Genome accession   NZ_CP093310
Coordinates   186604..187866 (-) Length   420 a.a.
NCBI ID   WP_338412388.1    Uniprot ID   A0AAU6PV60
Organism   Psychrobacter raelei strain PraFG1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 181604..192866
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN210_RS00775 (MN210_00985) - 181626..181958 (+) 333 WP_011959442.1 pyrimidine/purine nucleoside phosphorylase -
  MN210_RS00780 (MN210_00990) rlmB 182161..182946 (+) 786 WP_338412386.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MN210_RS00785 (MN210_00995) coaE 183050..183757 (-) 708 WP_083757352.1 dephospho-CoA kinase -
  MN210_RS00790 (MN210_01000) - 184297..185217 (-) 921 WP_338412387.1 A24 family peptidase -
  MN210_RS00795 (MN210_01005) pilC 185301..186461 (-) 1161 WP_338412855.1 type II secretion system F family protein Machinery gene
  MN210_RS00800 (MN210_01010) pilB 186604..187866 (-) 1263 WP_338412388.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  MN210_RS00805 (MN210_01015) tpiA 188202..188999 (+) 798 WP_201545875.1 triose-phosphate isomerase -
  MN210_RS00810 (MN210_01020) secG 189165..189482 (+) 318 WP_011959449.1 preprotein translocase subunit SecG -
  MN210_RS00825 (MN210_01035) rimP 190439..190939 (+) 501 WP_241878916.1 ribosome maturation factor RimP -
  MN210_RS00830 (MN210_01040) nusA 191231..192718 (+) 1488 WP_011959451.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45771.24 Da        Isoelectric Point: 6.8578

>NTDB_id=663444 MN210_RS00800 WP_338412388.1 186604..187866(-) (pilB) [Psychrobacter raelei strain PraFG1]
MSRPPSLNQLAAAPSATLAQAEHGAHQPHDLQQDPVVQLVQDLLCQAVDKGASDLHFEPYEHTFRVRFRIDGVMQQVATP
ALELQAKICARLKIMAQMDISERRMPQDGRIRLQLNGMPTLNFRANALPTLFGEKIVLRVINPAASIAGIDSLGLEPEQK
QQFLQALQQPQGILLITGPTGSGKTLSLYTGLDILNTVATNICTVEDPVEINLTGINQVTVNPKVGLSFAVALKSFLRQD
PDVLMVGEIRDFETADTAIKAAQTGHLVLSTLHTNSAAETLTRLHHMGVAYFNIATAISLVVAQRLARCLCRHCKIPCVV
PKVSLIEIGFSAEELAQPEHIIYKAVGCDKCCEGYKGRIGVFEVMPMNTALAQMIMQGANAIEIKAAATEQGFFDLRRAA
ILKVLQGILSIEEMTRMTAQ

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=663444 MN210_RS00800 WP_338412388.1 186604..187866(-) (pilB) [Psychrobacter raelei strain PraFG1]
ATGTCCCGGCCCCCTTCGCTTAATCAACTAGCTGCCGCTCCCTCTGCGACTCTAGCACAGGCAGAGCATGGCGCGCATCA
GCCTCATGATTTACAGCAAGATCCGGTAGTTCAGCTGGTACAGGATTTATTGTGTCAGGCAGTAGATAAAGGCGCCTCTG
ACCTACACTTTGAGCCCTATGAGCATACGTTTCGGGTACGCTTTCGTATCGATGGCGTCATGCAGCAAGTGGCCACACCA
GCGCTTGAGCTGCAAGCTAAGATATGTGCCCGCCTAAAGATTATGGCACAAATGGACATATCTGAGCGGCGTATGCCTCA
AGATGGCCGTATTAGGCTGCAACTAAATGGGATGCCGACACTAAACTTTCGTGCCAATGCTCTACCCACTTTGTTCGGTG
AAAAAATTGTGCTTCGGGTTATTAATCCAGCCGCCTCTATCGCTGGTATTGATAGCCTCGGGCTTGAGCCTGAGCAAAAA
CAGCAGTTTTTACAAGCGCTACAGCAGCCGCAAGGTATCTTATTAATTACCGGCCCCACAGGCTCTGGCAAAACCTTATC
GCTCTATACCGGTCTAGATATTTTAAACACGGTGGCCACCAATATCTGCACTGTAGAAGATCCGGTTGAAATTAATTTAA
CAGGTATCAATCAGGTCACCGTCAATCCTAAAGTCGGACTGAGCTTTGCGGTGGCCTTAAAGTCATTTTTGCGCCAAGAC
CCCGATGTATTAATGGTGGGTGAGATACGTGATTTTGAAACCGCGGATACTGCCATTAAAGCGGCACAAACTGGCCATTT
GGTGCTCTCTACCCTGCACACCAACTCAGCTGCTGAAACCTTAACTCGCCTGCATCATATGGGCGTAGCTTACTTTAATA
TTGCTACCGCCATTAGTCTGGTAGTGGCGCAGCGTCTGGCAAGATGCTTGTGCCGCCACTGCAAAATCCCTTGTGTGGTT
CCTAAGGTAAGTCTCATTGAGATTGGCTTTAGCGCCGAAGAGTTGGCACAGCCTGAGCATATCATCTATAAGGCGGTGGG
CTGTGATAAGTGCTGTGAGGGTTACAAAGGGCGCATCGGTGTGTTTGAGGTGATGCCCATGAACACGGCGCTTGCACAAA
TGATTATGCAAGGTGCTAATGCCATTGAGATAAAAGCGGCTGCCACCGAGCAAGGCTTTTTTGACTTAAGGCGGGCCGCT
ATTTTGAAAGTGCTTCAAGGCATCCTTAGCATTGAAGAAATGACCCGCATGACGGCCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-308)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

62.435

91.905

0.574
  pilB Acinetobacter baumannii D1279779

60.881

91.905

0.56
  pilB Vibrio cholerae strain A1552

55.216

93.571

0.517
  pilF Neisseria gonorrhoeae MS11

54.987

93.095

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

53.061

93.333

0.495
  pilB Legionella pneumophila strain ERS1305867

53.766

91.667

0.493
  pilB Vibrio campbellii strain DS40M4

52.284

93.81

0.49
  pilB/pilB1 Synechocystis sp. PCC 6803

43.659

97.619

0.426
  pilF Thermus thermophilus HB27

42.68

95.952

0.41
  pilB Haemophilus influenzae Rd KW20

40.338

98.571

0.398
  pilB Haemophilus influenzae 86-028NP

40.097

98.571

0.395
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.969

91.905

0.386