Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MOO40_RS03420 Genome accession   NZ_CP093308
Coordinates   702469..703578 (-) Length   369 a.a.
NCBI ID   WP_017411211.1    Uniprot ID   A0A1C2L453
Organism   Aeromonas hydrophila strain AC185     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 697469..708578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MOO40_RS03400 (MOO40_03405) brnQ 697927..699234 (-) 1308 WP_011707394.1 branched-chain amino acid transport system II carrier protein -
  MOO40_RS03405 (MOO40_03410) - 699425..700135 (-) 711 WP_219280850.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  MOO40_RS03410 (MOO40_03415) srmB 700264..701487 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  MOO40_RS03415 (MOO40_03420) yaaA 701664..702437 (-) 774 WP_241871763.1 peroxide stress protein YaaA -
  MOO40_RS03420 (MOO40_03425) pilU 702469..703578 (-) 1110 WP_017411211.1 type IVa pilus ATPase TapU Machinery gene
  MOO40_RS03425 (MOO40_03430) pilT 703607..704641 (-) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  MOO40_RS03430 (MOO40_03435) - 704681..705382 (+) 702 WP_029304817.1 YggS family pyridoxal phosphate-dependent enzyme -
  MOO40_RS03435 (MOO40_03440) proC 705441..706265 (+) 825 WP_241871764.1 pyrroline-5-carboxylate reductase -
  MOO40_RS03440 (MOO40_03445) - 706306..706857 (+) 552 WP_016351969.1 YggT family protein -
  MOO40_RS03445 (MOO40_03450) yggU 706857..707156 (+) 300 WP_045525470.1 DUF167 family protein YggU -
  MOO40_RS03450 (MOO40_03455) - 707175..707594 (+) 420 WP_045525468.1 DUF4426 domain-containing protein -
  MOO40_RS03455 (MOO40_03460) - 707649..707960 (-) 312 WP_024945836.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41035.17 Da        Isoelectric Point: 6.5035

>NTDB_id=663395 MOO40_RS03420 WP_017411211.1 702469..703578(-) (pilU) [Aeromonas hydrophila strain AC185]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=663395 MOO40_RS03420 WP_017411211.1 702469..703578(-) (pilU) [Aeromonas hydrophila strain AC185]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCATCTGCAGTCGCTGGCAGAAGCGCCCCTCGACAAGGCAAGGGCGCTGGCACTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAGCGCTACCTGCACACCAAGGAGGCCAACTACGCCATTCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATTGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
AGCGCTGAAGAGTTCGCTGCGCCAGGCCCCTGACGTGATCCTGATCGGTGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACATCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGACGCCGTCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCG
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
TTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C2L453

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36