Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MLC69_RS04170 Genome accession   NZ_CP092830
Coordinates   920386..921594 (-) Length   402 a.a.
NCBI ID   WP_078046826.1    Uniprot ID   -
Organism   Pseudomonas azotoformans strain ZY-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 915386..926594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MLC69_RS04130 (MLC69_04130) - 916303..916476 (-) 174 WP_016976295.1 DUF3094 family protein -
  MLC69_RS04135 (MLC69_04135) - 916531..917160 (-) 630 WP_010213242.1 DUF1780 domain-containing protein -
  MLC69_RS04140 (MLC69_04140) - 917264..917731 (+) 468 WP_078046822.1 FAD/FMN-containing dehydrogenase -
  MLC69_RS04145 (MLC69_04145) - 917728..918417 (+) 690 WP_078046823.1 energy-coupling factor ABC transporter permease -
  MLC69_RS04150 (MLC69_04150) - 918494..918709 (+) 216 WP_003171680.1 hypothetical protein -
  MLC69_RS04155 (MLC69_04155) yacG 918706..918906 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  MLC69_RS04160 (MLC69_04160) coaE 918903..919526 (-) 624 WP_078046824.1 dephospho-CoA kinase -
  MLC69_RS04165 (MLC69_04165) pilD 919523..920389 (-) 867 WP_078046825.1 A24 family peptidase Machinery gene
  MLC69_RS04170 (MLC69_04170) pilC 920386..921594 (-) 1209 WP_078046826.1 type II secretion system F family protein Machinery gene
  MLC69_RS04175 (MLC69_04175) - 921797..922210 (+) 414 WP_078050867.1 pilin -
  MLC69_RS04180 (MLC69_04180) - 922246..923646 (+) 1401 WP_078046827.1 O-antigen ligase family protein -
  MLC69_RS04190 (MLC69_04190) - 923928..924233 (+) 306 WP_078046828.1 DUF6388 family protein -
  MLC69_RS04195 (MLC69_04195) nadC 924277..925125 (-) 849 WP_078046829.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43301.79 Da        Isoelectric Point: 10.0815

>NTDB_id=660628 MLC69_RS04170 WP_078046826.1 920386..921594(-) (pilC) [Pseudomonas azotoformans strain ZY-1]
MDNASTLYAWEGTNRKGRRVSGRTTGHNPAWVKAQLRKQGISPGHVRQKSPMLPSLAPSIISTDITLLTRQLATLLKAGI
PLLQAFDIIGEGFDNRAVRELVQGLKQAIAAGTSLAEALRKQPRYFDALYCNLVAAGEQAGALETLLERVAIHREKSEQL
KARIKKAMTYPITVLVVASLVTGVLLIHVVPQFQNLFAGVDGTLPGFTLQVIALSEFVQKAWWVLALGVLAGSMGLRHAY
RTLPGFCLWLDRCLLKAPLAGKLLSKSAVARFARTLSTTFAAGVPLLQALDSVAGAAGSGPFRQAIEHMRRDVATGMPLN
QSMLASGLFPGMAIQMTAIGEESGTLDRMLEKVANHYEADVDNLVDNLTHLMEPLIMVVLGGIVGALVIAMYLPVFQMGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=660628 MLC69_RS04170 WP_078046826.1 920386..921594(-) (pilC) [Pseudomonas azotoformans strain ZY-1]
ATGGACAACGCTTCAACGCTCTACGCCTGGGAAGGCACCAACCGCAAAGGACGCAGGGTGTCCGGGCGAACCACTGGGCA
CAACCCTGCCTGGGTGAAGGCCCAATTGCGCAAACAGGGCATCAGCCCAGGCCATGTGCGCCAGAAATCTCCAATGCTGC
CGAGCCTGGCACCCTCGATAATATCGACGGACATCACCCTGCTTACTCGCCAACTGGCCACCCTGCTGAAGGCCGGTATT
CCTCTATTGCAGGCCTTCGATATCATCGGTGAGGGCTTCGACAACCGCGCCGTGCGTGAGCTTGTGCAAGGGTTGAAACA
GGCGATCGCCGCCGGCACCAGCCTTGCCGAGGCGCTGCGCAAACAGCCTCGTTATTTCGACGCGCTGTACTGCAACCTGG
TGGCCGCCGGCGAACAGGCCGGAGCACTTGAAACCCTGCTGGAGCGCGTGGCGATCCACCGGGAAAAGAGCGAGCAGCTA
AAGGCCAGGATCAAAAAAGCCATGACCTATCCGATCACCGTGCTGGTGGTCGCCAGCCTGGTCACTGGGGTGCTGTTGAT
CCACGTCGTGCCGCAGTTCCAGAATCTGTTTGCGGGGGTGGACGGCACGCTGCCCGGCTTCACCTTGCAGGTCATCGCCC
TTTCCGAGTTCGTGCAGAAAGCGTGGTGGGTGCTGGCGCTGGGCGTTCTTGCGGGAAGCATGGGGCTGCGCCATGCCTAT
CGGACGCTCCCTGGATTTTGTCTGTGGTTGGACCGCTGTTTGTTGAAAGCGCCCCTGGCAGGCAAACTGCTGAGCAAATC
CGCCGTGGCCCGCTTTGCCCGCACACTGTCCACCACGTTTGCCGCCGGCGTTCCGTTACTGCAGGCGCTGGACTCGGTGG
CCGGTGCCGCCGGTTCCGGGCCATTCAGACAGGCAATCGAACATATGCGACGCGATGTAGCGACTGGCATGCCATTGAAT
CAATCCATGCTCGCCAGCGGCCTGTTCCCCGGCATGGCGATCCAGATGACGGCCATCGGCGAAGAGTCGGGCACGCTGGA
CCGCATGCTGGAAAAGGTTGCCAACCATTACGAGGCCGATGTGGACAACCTGGTCGATAACCTCACGCACCTCATGGAGC
CCCTGATCATGGTGGTGCTGGGCGGCATTGTCGGCGCATTGGTGATCGCCATGTACCTGCCGGTCTTCCAGATGGGGAGC
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.596

98.507

0.587
  pilC Acinetobacter baumannii D1279779

51.392

98.259

0.505
  pilC Acinetobacter baylyi ADP1

50.758

98.507

0.5
  pilC Legionella pneumophila strain ERS1305867

48.485

98.507

0.478
  pilC Vibrio cholerae strain A1552

41.414

98.507

0.408
  pilG Neisseria gonorrhoeae MS11

40.704

99.005

0.403
  pilG Neisseria meningitidis 44/76-A

40.302

98.756

0.398
  pilC Vibrio campbellii strain DS40M4

39.086

98.01

0.383
  pilC Thermus thermophilus HB27

37.093

99.254

0.368