Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MLC69_RS02170 Genome accession   NZ_CP092830
Coordinates   476282..477607 (+) Length   441 a.a.
NCBI ID   WP_078046507.1    Uniprot ID   -
Organism   Pseudomonas azotoformans strain ZY-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 471282..482607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MLC69_RS02150 (MLC69_02150) - 471360..473822 (-) 2463 WP_078046503.1 penicillin-binding protein 1A -
  MLC69_RS02155 (MLC69_02155) pilM 474040..474693 (+) 654 WP_078046504.1 type IV pilus assembly protein PilM -
  MLC69_RS02160 (MLC69_02160) - 474690..475244 (+) 555 WP_078046505.1 PilN domain-containing protein -
  MLC69_RS02165 (MLC69_02165) - 475241..476212 (+) 972 WP_078046506.1 pilus assembly protein PilP -
  MLC69_RS02170 (MLC69_02170) pilQ 476282..477607 (+) 1326 WP_078046507.1 type IV pilus secretin PilQ family protein Machinery gene
  MLC69_RS02175 (MLC69_02175) aroK 477612..478130 (+) 519 WP_003187914.1 shikimate kinase AroK -
  MLC69_RS02180 (MLC69_02180) aroB 478256..479356 (+) 1101 WP_078046508.1 3-dehydroquinate synthase -
  MLC69_RS02185 (MLC69_02185) - 479367..480926 (+) 1560 WP_078046509.1 AAA family ATPase -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47789.63 Da        Isoelectric Point: 4.8179

>NTDB_id=660626 MLC69_RS02170 WP_078046507.1 476282..477607(+) (pilQ) [Pseudomonas azotoformans strain ZY-1]
MAAALPNRVDLIQLPPPGNTASSAYTGDRLNLNFQSIELRTALQQIADVAGLNLVASDDVQGSITLRLKDVPWDQALDLV
LQAKGLDKRVKAGVLLVAPAEELAARELLTLESRKQMAELAPLRRELLQVNYAKAADLAKLFQSVTGLEGVPDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGARWGGRLNRGNWGAGGIAKPPAEGAQPSE
NPPSSPFVDLGSLTGTSGLGIAFITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESSSSGATSV
SFKEASLSLEVTPQITPDDRVIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=660626 MLC69_RS02170 WP_078046507.1 476282..477607(+) (pilQ) [Pseudomonas azotoformans strain ZY-1]
ATGGCTGCTGCTCTACCTAATCGCGTAGACCTGATCCAGTTGCCACCGCCTGGCAATACCGCGTCGAGCGCCTACACGGG
TGACAGGCTCAACCTCAACTTCCAAAGCATCGAGCTGCGTACGGCGTTGCAACAAATCGCCGATGTGGCGGGCCTCAACC
TGGTGGCGAGCGACGATGTGCAGGGTTCAATTACCCTGCGCCTCAAGGATGTGCCCTGGGATCAGGCGCTGGACCTGGTG
CTGCAGGCCAAGGGCCTGGATAAGCGGGTGAAGGCCGGCGTGTTACTGGTCGCGCCCGCCGAAGAGTTGGCTGCACGTGA
GCTGCTGACCCTGGAGTCGCGCAAGCAAATGGCCGAGCTGGCGCCATTGCGCCGTGAACTCTTGCAAGTCAATTACGCCA
AGGCGGCAGACCTGGCCAAGCTGTTCCAGTCGGTGACGGGCCTTGAAGGCGTGCCCGACGAGCGTGGGTCGGTGGCGGTC
GATGATCGCACCAATAACATCATCGCTTACCAGACCGGGGAGCGGCTGGAGGAGCTGCGGCGGATCGTGGCGCAACTGGA
CATCCCCGTGCGACAGGTGATGATCGAGGCGCGCATTGTCGAGGCCAATGTCGATTACGACAAAAGCCTGGGCGCGCGCT
GGGGCGGACGACTCAATCGCGGCAACTGGGGCGCCGGGGGGATTGCGAAGCCACCGGCCGAAGGTGCGCAGCCTTCGGAA
AACCCGCCCAGCTCACCCTTTGTTGACTTGGGCTCGCTCACCGGCACCTCGGGCCTGGGCATCGCCTTTATCACCGACAA
CCTGCTGCTGGACCTGGAGCTCACCGCCATGGAGAAAACCGGCAACGGGGAAATTGTCTCGCAACCCAAGGTGGTGACTT
CCGACAAGGAAACTGCGCGCATCCTCAAGGGCACTGAAATCCCCTATCAGGAATCCAGCTCCAGCGGCGCCACGTCGGTG
TCGTTCAAGGAAGCCTCGTTGTCGCTGGAGGTGACCCCGCAGATCACCCCCGACGACCGGGTGATCATGGAAGTCAAAGT
CACCAAGGACGAGCCCGATTACCTGAACAAGCTCAATGACGTGCCACCGATCAAGAAAAACGAAGTGAACGCCAAGGTGC
TGGTCAAGGATGGCGAGACCATCGTCATCGGCGGCGTTTTCTCTAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(53-99)

Bacterial type II and III secretion system protein (Secretin)

(276-432)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.754

99.546

0.714
  comQ Acinetobacter baylyi ADP1

42

100

0.429
  pilQ Acinetobacter baumannii D1279779

40.517

100

0.426
  pilQ Acinetobacter baumannii strain A118

40.517

100

0.426
  pilQ Legionella pneumophila strain Lp02

44.181

95.465

0.422
  pilQ Vibrio cholerae strain A1552

44.391

95.011

0.422
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

44.391

95.011

0.422
  pilQ Vibrio campbellii strain DS40M4

43.735

95.918

0.419
  pilQ Legionella pneumophila strain ERS1305867

43.705

95.465

0.417
  pilQ Neisseria gonorrhoeae strain FA1090

36.384

99.093

0.361