Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MJ904_RS11575 Genome accession   NZ_CP092780
Coordinates   2536693..2537883 (+) Length   396 a.a.
NCBI ID   WP_050409374.1    Uniprot ID   A0A0K1JZF0
Organism   Massilia sp. MB5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2531693..2542883
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ904_RS11550 (MJ904_11550) proC 2532363..2533200 (+) 838 Protein_2280 pyrroline-5-carboxylate reductase -
  MJ904_RS11555 (MJ904_11555) - 2533365..2534180 (+) 816 WP_240737495.1 transporter substrate-binding domain-containing protein -
  MJ904_RS11560 (MJ904_11560) - 2534265..2534636 (-) 372 WP_082219531.1 YqjD family protein -
  MJ904_RS11565 (MJ904_11565) ubiA 2534720..2535574 (-) 855 WP_240737852.1 4-hydroxybenzoate octaprenyltransferase -
  MJ904_RS11570 (MJ904_11570) - 2535648..2536589 (-) 942 WP_050409375.1 hydrogen peroxide-inducible genes activator -
  MJ904_RS11575 (MJ904_11575) pilU 2536693..2537883 (+) 1191 WP_050409374.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MJ904_RS11580 (MJ904_11580) rfaE2 2537896..2538378 (+) 483 WP_176349188.1 D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase -
  MJ904_RS11585 (MJ904_11585) trmL 2538395..2538865 (-) 471 WP_050409372.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  MJ904_RS11590 (MJ904_11590) - 2538875..2539711 (-) 837 WP_240737496.1 ComF family protein -
  MJ904_RS11595 (MJ904_11595) - 2539770..2540695 (+) 926 Protein_2289 methyltransferase domain-containing protein -
  MJ904_RS11600 (MJ904_11600) coxB 2541068..2542296 (+) 1229 Protein_2290 cytochrome c oxidase subunit II -

Sequence

Protein


Download         Length: 396 a.a.        Molecular weight: 43923.06 Da        Isoelectric Point: 6.1525

>NTDB_id=660281 MJ904_RS11575 WP_050409374.1 2536693..2537883(+) (pilU) [Massilia sp. MB5]
MSSIFGHEEAQAYMHKLLKAMHQVGGSDLFISADFPPSIKSQGAMKALSQQRLSGEVTRALASAIMNEKQRQEFEAELEC
NFAISLPDVCRFRVNVFVQQQNVAMVIRTIASEIPNFDKLDLPEVLKDVVMTKRGLVLVVGGTGSGKSTTLAAMIDYRNG
HSAGHIITVEDPVEYVHKNKGCLVTHREVGVDTHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTMDRIINFFPEERRNQLLMDLSSNLRAIVSQRLIRTEDGKGRKAAIEILLNTPTIAEMIFKGSFQTIKEIMHKS
RELGMCTFDQALYELYNKGYISYEEALRNADSANGLRLQIKLRGDRKEPGGAAAAGGAAELSMQMDEEDEEEPGSH

Nucleotide


Download         Length: 1191 bp        

>NTDB_id=660281 MJ904_RS11575 WP_050409374.1 2536693..2537883(+) (pilU) [Massilia sp. MB5]
ATGTCGTCGATTTTTGGCCACGAAGAAGCACAGGCATATATGCACAAGCTGCTAAAAGCCATGCACCAGGTGGGCGGTTC
GGACTTGTTCATCTCGGCCGACTTCCCGCCCAGCATCAAATCCCAGGGCGCGATGAAGGCGCTCAGCCAGCAGCGCTTGA
GCGGCGAGGTGACGCGCGCCCTGGCCTCCGCCATCATGAACGAGAAGCAGCGCCAGGAGTTCGAGGCTGAGCTCGAATGC
AACTTCGCCATCTCGCTGCCGGACGTGTGCCGCTTTCGCGTCAACGTCTTCGTGCAGCAGCAGAATGTGGCCATGGTGAT
CCGCACCATCGCCTCCGAAATCCCGAACTTCGACAAGCTCGACCTACCCGAGGTGCTGAAAGACGTGGTGATGACCAAGC
GCGGCCTGGTGCTGGTGGTGGGCGGCACCGGTTCCGGCAAATCGACCACCTTGGCGGCCATGATCGACTACCGCAACGGC
CACTCGGCCGGCCACATCATCACGGTGGAAGACCCGGTCGAATACGTGCACAAGAACAAGGGCTGCCTGGTGACGCACCG
CGAAGTGGGCGTGGACACGCACAGCTGGCACAACGCGCTGAAGAACACCTTGCGCCAGGCGCCGGATGTGATCCTGATCG
GCGAAATCCGCGATACCGAAACCATGGAGCATGCCATCGCCTTCGCCGAAACCGGCCACCTCTGCCTGGGCACCCTGCAC
GCGAACAACGCCAACCAGACCATGGACCGCATCATCAACTTCTTTCCGGAAGAGCGGCGCAACCAGCTGCTGATGGACCT
GTCCTCGAACCTGCGCGCCATCGTCTCGCAGCGCCTGATCCGCACCGAGGACGGCAAAGGGCGCAAGGCCGCCATTGAAA
TCTTGCTGAACACGCCGACGATTGCCGAGATGATTTTCAAGGGCAGCTTCCAGACCATCAAGGAAATCATGCACAAATCG
CGCGAGCTGGGCATGTGCACTTTCGACCAGGCGCTGTACGAACTTTATAATAAGGGCTACATCTCATACGAAGAAGCGCT
GCGCAATGCCGACTCGGCCAATGGCCTGCGTCTGCAGATCAAGCTGCGCGGCGACCGCAAGGAGCCAGGCGGCGCTGCCG
CTGCCGGCGGCGCGGCCGAACTGAGCATGCAGATGGACGAGGAAGACGAAGAAGAACCCGGCTCACACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K1JZF0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.728

95.707

0.543
  pilU Acinetobacter baylyi ADP1

54.52

89.394

0.487
  pilU Vibrio cholerae strain A1552

52.125

89.141

0.465
  pilT Pseudomonas aeruginosa PAK

44.478

84.596

0.376
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.713

84.343

0.369
  pilT Acinetobacter baylyi ADP1

43.807

83.586

0.366
  pilT Legionella pneumophila strain Lp02

45.171

81.061

0.366
  pilT Legionella pneumophila strain ERS1305867

45.171

81.061

0.366
  pilT Pseudomonas stutzeri DSM 10701

42.985

84.596

0.364