Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MKR81_RS02860 Genome accession   NZ_CP092682
Coordinates   582450..582863 (-) Length   137 a.a.
NCBI ID   WP_045395349.1    Uniprot ID   -
Organism   Vibrio campbellii strain IFL1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 577450..587863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKR81_RS02835 (MKR81_02835) coaE 577520..578134 (-) 615 WP_012128880.1 dephospho-CoA kinase -
  MKR81_RS02840 (MKR81_02840) pilD 578135..579004 (-) 870 WP_240305019.1 A24 family peptidase Machinery gene
  MKR81_RS02845 (MKR81_02845) pilC 579077..580300 (-) 1224 WP_240305020.1 type II secretion system F family protein Machinery gene
  MKR81_RS02850 (MKR81_02850) pilB 580335..582020 (-) 1686 WP_240305021.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MKR81_RS02855 (MKR81_02855) - 582022..582450 (-) 429 WP_240305022.1 pilin -
  MKR81_RS02860 (MKR81_02860) pilA 582450..582863 (-) 414 WP_045395349.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MKR81_RS02865 (MKR81_02865) nadC 583129..584016 (-) 888 WP_012128875.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MKR81_RS02870 (MKR81_02870) ampD 584109..584660 (+) 552 WP_038891990.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MKR81_RS02875 (MKR81_02875) pdhR 585065..585832 (+) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14059.15 Da        Isoelectric Point: 8.4859

>NTDB_id=659666 MKR81_RS02860 WP_045395349.1 582450..582863(-) (pilA) [Vibrio campbellii strain IFL1]
MKTNKQKKQQGFTLIELMIVVAIIGVLAAIAIPAYKDYVVKGEASSALATLKALQAPAELQYQQDGALTTLAALGTTSGA
NSLGTLSVDSAVSAGLAFQFNSGTLNSTTMKLTRSATGWACSRTGATLTAIDLDGCN

Nucleotide


Download         Length: 414 bp        

>NTDB_id=659666 MKR81_RS02860 WP_045395349.1 582450..582863(-) (pilA) [Vibrio campbellii strain IFL1]
ATGAAAACGAATAAACAGAAAAAGCAGCAAGGTTTTACGCTGATTGAATTAATGATTGTCGTGGCGATTATTGGTGTTTT
GGCAGCAATAGCAATTCCTGCTTATAAAGATTACGTTGTAAAAGGTGAGGCATCTTCAGCTCTTGCAACGTTAAAAGCAT
TACAAGCTCCAGCAGAACTTCAGTATCAACAAGATGGAGCTCTTACAACGCTAGCAGCTCTTGGGACGACGTCAGGAGCA
AACTCTCTAGGCACTTTATCTGTCGATAGTGCGGTGTCAGCAGGTTTAGCTTTCCAATTCAATAGTGGTACGTTGAACTC
AACCACAATGAAGTTGACACGTTCCGCAACCGGTTGGGCATGTTCTCGTACTGGTGCAACATTAACAGCGATCGACCTCG
ATGGATGTAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

54.861

100

0.577
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.597

100

0.431
  pilA Vibrio cholerae strain A1552

39.597

100

0.431
  pilA Vibrio cholerae C6706

39.597

100

0.431
  pilA Pseudomonas aeruginosa PAK

39.259

98.54

0.387
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.394

96.35

0.38