Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MKR81_RS02850 Genome accession   NZ_CP092682
Coordinates   580335..582020 (-) Length   561 a.a.
NCBI ID   WP_240305021.1    Uniprot ID   -
Organism   Vibrio campbellii strain IFL1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 575335..587020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKR81_RS02820 (MKR81_02820) rplS 575674..576027 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  MKR81_RS02825 (MKR81_02825) yacG 576495..576695 (-) 201 WP_012128882.1 DNA gyrase inhibitor YacG -
  MKR81_RS02830 (MKR81_02830) zapD 576752..577492 (-) 741 WP_012128881.1 cell division protein ZapD -
  MKR81_RS02835 (MKR81_02835) coaE 577520..578134 (-) 615 WP_012128880.1 dephospho-CoA kinase -
  MKR81_RS02840 (MKR81_02840) pilD 578135..579004 (-) 870 WP_240305019.1 A24 family peptidase Machinery gene
  MKR81_RS02845 (MKR81_02845) pilC 579077..580300 (-) 1224 WP_240305020.1 type II secretion system F family protein Machinery gene
  MKR81_RS02850 (MKR81_02850) pilB 580335..582020 (-) 1686 WP_240305021.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MKR81_RS02855 (MKR81_02855) - 582022..582450 (-) 429 WP_240305022.1 pilin -
  MKR81_RS02860 (MKR81_02860) pilA 582450..582863 (-) 414 WP_045395349.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MKR81_RS02865 (MKR81_02865) nadC 583129..584016 (-) 888 WP_012128875.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MKR81_RS02870 (MKR81_02870) ampD 584109..584660 (+) 552 WP_038891990.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MKR81_RS02875 (MKR81_02875) pdhR 585065..585832 (+) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62051.85 Da        Isoelectric Point: 5.1024

>NTDB_id=659665 MKR81_RS02850 WP_240305021.1 580335..582020(-) (pilB) [Vibrio campbellii strain IFL1]
MITNLSTVLRQAGILTFSQEELLLEQAKASGISMPEALLSSGFFQSHELTEHLSSVFGLSCTSLNQYEYASLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRSLGQEKSGLKEINQDELAG
LVDIGEDEIESIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDSQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCRQPQEPNSQLQHIGITSTEQ
IFQANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGASVHELEKLAIANGMQTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=659665 MKR81_RS02850 WP_240305021.1 580335..582020(-) (pilB) [Vibrio campbellii strain IFL1]
GTGATAACAAACCTCTCAACAGTTCTTCGCCAAGCAGGGATACTCACCTTTTCCCAAGAAGAACTGTTGCTTGAGCAAGC
TAAAGCTTCTGGCATTTCGATGCCGGAGGCTTTACTCAGTTCTGGCTTTTTCCAATCTCATGAATTAACAGAACACCTAA
GCTCTGTATTCGGGCTTTCTTGCACTTCTCTTAACCAATACGAATACGCTTCGCTATGCCAAACGCTTGGCTTACGCGAT
CTCATCACTCGCCATAACGCTTTGCCGCTTAACCGCACCTCTTCAACTTTGATTCTTGCTGTTGCCGACCCAACAAACCT
TCAAGCCGAAGATGACTTTCGTTTTGCGACAGGTTTGCAAGTCGAACTTGTCCTCGCTGACTTTCGTGAGCTAACAGCAG
CTATTCGGCGTTTATATGGCCGATCACTTGGGCAAGAAAAATCCGGTCTTAAAGAAATCAACCAAGATGAACTGGCGGGT
TTGGTTGATATTGGTGAGGATGAAATTGAGAGCATCGAAGATCTCAGCCAAGACGAATCGCCGGTCAGTCGCTATATCAA
CCAAATTTTACTCGATGCTGTCCGTAAAGGGGCTTCCGATATTCACTTCGAGCCTTACGAAAATATGTACCGCGTGCGCT
TGCGTTGCGATGGCATTTTAATTGAAATTCAGCAGCCTCCGAGCCATTTAAGCCGTCGCCTCTCCGCTCGCATAAAAATC
TTATCTAAGCTTGATATCGCCGAGCGCCGTTTACCACAGGATGGACGCATCAAGCTCAAATTAAACCAAGACACTGCAAT
TGATATGCGTGTCTCCACACTACCAACTCTTTTTGGGGAAAAGATTGTTCTGCGTTTACTCGACAGCAGTTCTGCAGCAT
TAGATATCAATAAGCTCGGTTATAGCGACTCACAAAAACAACTTTATTTGGATGCCCTGCGCCGTCCGCAAGGTATGATC
CTGATGACAGGCCCAACAGGTAGCGGTAAAACGGTCTCGCTCTACACTGGATTGAGCATTCTGAATAAACCAGAGATCAA
TATCTCAACCGCTGAAGATCCCGTTGAGATTAACCTGCCCGGCATTAACCAAGTTCAAGTTCAGCCTAAGATCGGTTTTG
GTTTTGCCGAAGCGCTCCGCTCTTTCTTACGTCAAGACCCTGATGTAGTCATGGTGGGCGAGATCCGAGATCTCGACACA
GCAGAAATCGCGATCAAAGCATCACAGACTGGTCACTTAGTTCTCTCCACTTTGCACACGAACTCAGCTGCCGAGACTGT
CATTCGCTTGTCTAATATGGGCGTAGAAAGCTTTAATCTCGCCTCCTCTCTCAGTTTGATCATCGCCCAACGTTTGGCTA
GAAAACTTTGTCCATATTGTAGACAACCTCAAGAGCCAAACAGCCAACTTCAACATATTGGAATCACTAGTACCGAACAG
ATCTTTCAAGCTAATCCCGACGGTTGTAACGAGTGTACCCATGGTTATTCAGGGCGAACCGGCATTTACGAAGTGATGAA
GTTTGATGAATCCCTTTCAGAAGCACTCATCAAAGGCGCCTCGGTACACGAATTAGAAAAACTCGCCATTGCCAATGGTA
TGCAAACCCTACAAATGTCTGGCATCGAAAAACTCAAACAAGGCATTACCAGCTTCAGCGAATTGCAGCGCGTTCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

98.574

100

0.986
  pilB Vibrio parahaemolyticus RIMD 2210633

90.196

100

0.902
  pilB Vibrio cholerae strain A1552

73.488

100

0.736
  pilB Acinetobacter baumannii D1279779

51.252

92.513

0.474
  pilB Acinetobacter baylyi ADP1

48.893

96.613

0.472
  pilB Legionella pneumophila strain ERS1305867

48.524

96.613

0.469
  pilF Neisseria gonorrhoeae MS11

43.617

100

0.439
  pilB/pilB1 Synechocystis sp. PCC 6803

36.7

100

0.389
  pilF Thermus thermophilus HB27

37.011

100

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.185

100

0.362