Detailed information NTG0659284

Bioinformatically predicted

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: MKF35_RS12415
• Genome accession: NZ_CP092629
• Coordinates: 2684237..2685454 (-)
• Length: 405 a.a.
• NCBI ID: WP_003107299.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain HU20
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2679237..2690454

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MKF35_RS12370	-	2679421..2679870 (-)	450	WP_003161439.1	MOSC domain-containing protein	-
MKF35_RS12375	-	2679867..2680496 (-)	630	WP_003094668.1	DUF1780 domain-containing protein	-
MKF35_RS12380	-	2680629..2681054 (+)	426	WP_003116252.1	GNAT family N-acetyltransferase	-
MKF35_RS12385	-	2681051..2681521 (+)	471	WP_003094664.1	hypothetical protein	-
MKF35_RS12390	-	2681518..2682207 (+)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
MKF35_RS12395	-	2682313..2682522 (+)	210	WP_003094660.1	hypothetical protein	-
MKF35_RS12400	yacG	2682559..2682759 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
MKF35_RS12405	coaE	2682756..2683367 (-)	612	WP_047105677.1	dephospho-CoA kinase	-
MKF35_RS12410	pilD	2683364..2684236 (-)	873	WP_003107301.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
MKF35_RS12415	pilC	2684237..2685454 (-)	1218	WP_003107299.1	type II secretion system F family protein	Machinery gene
MKF35_RS12420	pilB	2685458..2687158 (-)	1701	WP_003122078.1	type IV-A pilus assembly ATPase PilB	Machinery gene
MKF35_RS12425	-	2687389..2687853 (+)	465	WP_016264268.1	pilin	-
MKF35_RS12430	-	2687935..2689314 (+)	1380	WP_229027906.1	O-antigen ligase family protein	-
MKF35_RS12440	nadC	2689494..2690342 (-)	849	WP_003161760.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 405 a.a.
• Molecular weight: 44001.86 Da
• Isoelectric Point: 9.8103

>NTDB_id=659284 MKF35_RS12415 WP_003107299.1 2684237..2685454(-) (pilC) [Pseudomonas aeruginosa strain HU20]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTMMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide

• Length: 1218 bp

>NTDB_id=659284 MKF35_RS12415 WP_003107299.1 2684237..2685454(-) (pilC) [Pseudomonas aeruginosa strain HU20]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAGAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TCTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCGCTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCGATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCAGCCTTCACCATGATGG
TCATCAACATTTCCAACGTACTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCTATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTGTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAGTCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (276-397)

• Type II secretion system (T2SS), protein F (T2SSF) (71-194)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilC	Pseudomonas stutzeri DSM 10701	78.765	100	0.788
pilC	Acinetobacter baylyi ADP1	61.029	100	0.615
pilC	Acinetobacter baumannii D1279779	60.396	99.753	0.602
pilC	Legionella pneumophila strain ERS1305867	54.293	97.778	0.531
pilG	Neisseria gonorrhoeae MS11	46.04	99.753	0.459
pilG	Neisseria meningitidis 44/76-A	45.522	99.259	0.452
pilC	Vibrio cholerae strain A1552	41.481	100	0.415
pilC	Vibrio campbellii strain DS40M4	40.648	99.012	0.402
pilC	Thermus thermophilus HB27	38.155	99.012	0.378