Detailed information NTG0659283

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MKF35_RS12410
• Genome accession: NZ_CP092629
• Coordinates: 2683364..2684236 (-)
• Length: 290 a.a.
• NCBI ID: WP_003107301.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain HU20
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2678364..2689236

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MKF35_RS12365	-	2679225..2679395 (-)	171	WP_003094672.1	DUF3094 family protein	-
MKF35_RS12370	-	2679421..2679870 (-)	450	WP_003161439.1	MOSC domain-containing protein	-
MKF35_RS12375	-	2679867..2680496 (-)	630	WP_003094668.1	DUF1780 domain-containing protein	-
MKF35_RS12380	-	2680629..2681054 (+)	426	WP_003116252.1	GNAT family N-acetyltransferase	-
MKF35_RS12385	-	2681051..2681521 (+)	471	WP_003094664.1	hypothetical protein	-
MKF35_RS12390	-	2681518..2682207 (+)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
MKF35_RS12395	-	2682313..2682522 (+)	210	WP_003094660.1	hypothetical protein	-
MKF35_RS12400	yacG	2682559..2682759 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
MKF35_RS12405	coaE	2682756..2683367 (-)	612	WP_047105677.1	dephospho-CoA kinase	-
MKF35_RS12410	pilD	2683364..2684236 (-)	873	WP_003107301.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
MKF35_RS12415	pilC	2684237..2685454 (-)	1218	WP_003107299.1	type II secretion system F family protein	Machinery gene
MKF35_RS12420	pilB	2685458..2687158 (-)	1701	WP_003122078.1	type IV-A pilus assembly ATPase PilB	Machinery gene
MKF35_RS12425	-	2687389..2687853 (+)	465	WP_016264268.1	pilin	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31873.90 Da
• Isoelectric Point: 7.7068

>NTDB_id=659283 MKF35_RS12410 WP_003107301.1 2683364..2684236(-) (pilD) [Pseudomonas aeruginosa strain HU20]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=659283 MKF35_RS12410 WP_003107301.1 2683364..2684236(-) (pilD) [Pseudomonas aeruginosa strain HU20]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCTTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.921	95.862	0.469