Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MIA32_RS03240 Genome accession   NZ_CP092356
Coordinates   689736..690845 (-) Length   369 a.a.
NCBI ID   WP_016351972.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain CCL1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 684736..695845
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIA32_RS03220 (MIA32_03220) brnQ 685193..686500 (-) 1308 WP_043119734.1 branched-chain amino acid transport system II carrier protein -
  MIA32_RS03225 (MIA32_03225) - 686692..687402 (-) 711 WP_016351975.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  MIA32_RS03230 (MIA32_03230) srmB 687531..688754 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  MIA32_RS03235 (MIA32_03235) yaaA 688931..689704 (-) 774 WP_016351973.1 peroxide stress protein YaaA -
  MIA32_RS03240 (MIA32_03240) pilU 689736..690845 (-) 1110 WP_016351972.1 type IVa pilus ATPase TapU Machinery gene
  MIA32_RS03245 (MIA32_03245) pilT 690874..691908 (-) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  MIA32_RS03250 (MIA32_03250) - 691948..692649 (+) 702 WP_016351971.1 YggS family pyridoxal phosphate-dependent enzyme -
  MIA32_RS03255 (MIA32_03255) proC 692708..693532 (+) 825 WP_016351970.1 pyrroline-5-carboxylate reductase -
  MIA32_RS03260 (MIA32_03260) - 693573..694124 (+) 552 WP_016351969.1 YggT family protein -
  MIA32_RS03265 (MIA32_03265) yggU 694124..694423 (+) 300 WP_011707385.1 DUF167 family protein YggU -
  MIA32_RS03270 (MIA32_03270) - 694442..694861 (+) 420 WP_011707384.1 DUF4426 domain-containing protein -
  MIA32_RS03275 (MIA32_03275) - 694916..695227 (-) 312 WP_016351968.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41054.21 Da        Isoelectric Point: 6.5657

>NTDB_id=656642 MIA32_RS03240 WP_016351972.1 689736..690845(-) (pilU) [Aeromonas hydrophila strain CCL1]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=656642 MIA32_RS03240 WP_016351972.1 689736..690845(-) (pilU) [Aeromonas hydrophila strain CCL1]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGTAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCACTGGCAGAAGCGCCCCTCGACAAGGCAAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAGCGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATTGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGTAGCCTGATCACCCAGCGCGAGGTAGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAGAGTTCGTTGCGCCAGGCCCCTGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAAACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAATCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGACGCCGTCGTTGTGCGGCCTTCGAGGTACTGCTCAACACCCCTCTCATCACCGATATCG
TCCGCAAGGGAGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36