Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LN244_RS07325 Genome accession   NZ_CP092123
Coordinates   1597487..1598554 (-) Length   355 a.a.
NCBI ID   WP_239042188.1    Uniprot ID   -
Organism   Marinobacterium sediminicola strain 1.7287     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1592487..1603554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LN244_RS07295 (LN244_07295) - 1593344..1593556 (+) 213 WP_239042182.1 hypothetical protein -
  LN244_RS07300 (LN244_07300) - 1593602..1594630 (+) 1029 WP_239042183.1 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase -
  LN244_RS07305 (LN244_07305) - 1594630..1595094 (+) 465 WP_239042184.1 histidine phosphatase family protein -
  LN244_RS07310 (LN244_07310) - 1595107..1595955 (+) 849 WP_239042185.1 alpha/beta fold hydrolase -
  LN244_RS07315 (LN244_07315) - 1595975..1596823 (+) 849 WP_239042186.1 DUF4892 domain-containing protein -
  LN244_RS07320 (LN244_07320) folE 1596852..1597394 (-) 543 WP_239042187.1 GTP cyclohydrolase I FolE -
  LN244_RS07325 (LN244_07325) pilU 1597487..1598554 (-) 1068 WP_239042188.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LN244_RS07330 (LN244_07330) - 1598630..1599202 (-) 573 WP_239042189.1 Smr/MutS family protein -
  LN244_RS07335 (LN244_07335) prmB 1599270..1600187 (+) 918 WP_239042190.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  LN244_RS07340 (LN244_07340) aroC 1600241..1601338 (+) 1098 WP_239042191.1 chorismate synthase -
  LN244_RS07345 (LN244_07345) speD 1601617..1602114 (+) 498 WP_239042192.1 adenosylmethionine decarboxylase -
  LN244_RS07350 (LN244_07350) speE 1602092..1602949 (+) 858 WP_239042193.1 polyamine aminopropyltransferase -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 39656.31 Da        Isoelectric Point: 7.0550

>NTDB_id=656036 LN244_RS07325 WP_239042188.1 1597487..1598554(-) (pilU) [Marinobacterium sediminicola strain 1.7287]
MLDLHKHLQLMSKTGASDLFLSAESPIMIKTEGVIKPVDDNRLSAESITELARSLMNEPQVRQFQEELEMNFAYDLPDTG
RFRVNVYRQRGNVAMVIRMIRHSIPSIESLNLPEALKQLAMLRSGLVLIVGATGSGKSTSMASMIEYRNQHASGHILTIE
DPIEFLHSNKKSLINQREVGVDTLSYANALKNALREAPDVIVIGEIRDAVTTEHAVRYSETGHLCLSTLHANNSVQAIEH
IANFFHDTDKHKVNNDLALNLKAIVSLRLIRDTHGHLIPATEVLINTPYIAKLIARGEYSRIREAIDQSSNSAAHTFDSN
LLNLYREGRITLEEALRNADSRNNVALRLKLMAGQ

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=656036 LN244_RS07325 WP_239042188.1 1597487..1598554(-) (pilU) [Marinobacterium sediminicola strain 1.7287]
ATGCTGGATCTGCATAAACACCTCCAACTGATGTCCAAAACCGGCGCTTCCGACCTCTTTCTGAGCGCCGAATCCCCCAT
AATGATCAAGACAGAAGGCGTCATCAAACCGGTTGATGACAACCGCCTGAGTGCAGAAAGCATCACCGAACTGGCCCGTT
CACTGATGAACGAACCTCAGGTTCGGCAGTTTCAGGAAGAACTTGAAATGAACTTCGCTTACGACCTACCGGATACCGGT
CGCTTCAGGGTTAACGTGTACCGCCAGCGCGGTAATGTTGCGATGGTTATTCGCATGATCCGCCACAGCATTCCAAGTAT
TGAGTCGCTCAACCTGCCCGAAGCTCTCAAACAGTTAGCTATGCTGAGAAGTGGCCTGGTCCTGATTGTCGGTGCCACAG
GGTCAGGCAAGTCAACCTCCATGGCCTCCATGATCGAATATCGCAATCAGCATGCCAGCGGCCACATACTAACCATCGAA
GATCCGATCGAGTTCTTGCACAGCAACAAAAAGTCCCTGATTAATCAGCGTGAGGTGGGTGTAGACACCCTCTCATATGC
CAACGCGTTGAAGAATGCACTGCGTGAGGCACCGGACGTCATTGTGATCGGAGAAATCCGTGATGCGGTGACTACCGAGC
ATGCGGTTCGTTACTCTGAAACAGGGCACCTGTGCCTGTCCACACTCCATGCCAACAACAGTGTTCAAGCGATAGAACAC
ATTGCAAATTTCTTCCATGACACCGACAAGCACAAAGTGAACAATGATCTCGCGCTCAACCTCAAGGCAATAGTATCTCT
GCGCCTGATCCGGGATACTCATGGCCACTTGATTCCTGCCACAGAGGTTTTGATCAATACTCCATATATCGCCAAGCTCA
TCGCTCGTGGTGAATATTCACGGATACGGGAAGCCATTGACCAATCAAGCAACAGTGCGGCACACACATTCGACAGCAAT
CTGCTTAACCTCTATCGTGAAGGCCGCATCACACTGGAAGAAGCATTGCGAAACGCCGATTCTCGCAACAATGTGGCACT
GAGACTCAAACTGATGGCGGGGCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.142

98.873

0.496
  pilU Acinetobacter baylyi ADP1

47.025

99.437

0.468
  pilU Vibrio cholerae strain A1552

44.915

99.718

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.765

95.775

0.4
  pilT Neisseria meningitidis 8013

39.003

96.056

0.375
  pilT Neisseria gonorrhoeae MS11

39.003

96.056

0.375
  pilT Pseudomonas aeruginosa PAK

38.192

96.62

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.039

93.803

0.366
  pilT Vibrio cholerae strain A1552

39.039

93.803

0.366