Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L3V47_RS02915 Genome accession   NZ_CP091480
Coordinates   604830..605939 (-) Length   369 a.a.
NCBI ID   WP_087757149.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain ASscau0313     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 599830..610939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3V47_RS02890 (L3V47_02885) - 599969..600199 (+) 231 WP_192896245.1 hypothetical protein -
  L3V47_RS02895 (L3V47_02890) brnQ 600275..601588 (-) 1314 WP_277735641.1 branched-chain amino acid transport system II carrier protein -
  L3V47_RS02900 (L3V47_02895) - 601774..602484 (-) 711 WP_096118856.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  L3V47_RS02905 (L3V47_02900) srmB 602613..603836 (+) 1224 WP_005320462.1 ATP-dependent RNA helicase SrmB -
  L3V47_RS02910 (L3V47_02905) yaaA 604025..604798 (-) 774 WP_125608785.1 peroxide stress protein YaaA -
  L3V47_RS02915 (L3V47_02910) pilU 604830..605939 (-) 1110 WP_087757149.1 type IVa pilus ATPase TapU Machinery gene
  L3V47_RS02920 (L3V47_02915) pilT 605975..607009 (-) 1035 WP_005320466.1 type IVa pilus ATPase TapT Machinery gene
  L3V47_RS02925 (L3V47_02920) - 607049..607750 (+) 702 WP_059169416.1 YggS family pyridoxal phosphate-dependent enzyme -
  L3V47_RS02930 (L3V47_02925) proC 607841..608665 (+) 825 WP_087757150.1 pyrroline-5-carboxylate reductase -
  L3V47_RS02935 (L3V47_02930) - 608694..609245 (+) 552 WP_005320477.1 YggT family protein -
  L3V47_RS02940 (L3V47_02935) yggU 609245..609544 (+) 300 WP_005320479.1 DUF167 family protein YggU -
  L3V47_RS02945 (L3V47_02940) - 609563..609982 (+) 420 WP_277735642.1 DUF4426 domain-containing protein -
  L3V47_RS02950 (L3V47_02945) - 610051..610350 (-) 300 WP_011899099.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41059.10 Da        Isoelectric Point: 6.3717

>NTDB_id=651619 L3V47_RS02915 WP_087757149.1 604830..605939(-) (pilU) [Aeromonas salmonicida strain ASscau0313]
MNLDALLAELVARKGSDLFITVGTPPTLKINGHLFSLGSEALDKQSALTLVRETLSPEHFERYIRTKEGNYAIHREALGR
FRVSAFWQQELPGMVVRRIETRIPTFDELTLPPILQEVAMAKRGLVLFVGATGTGKSTTQAAMIGYRNQNADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQLLFDLSFNLRAIVAQQLVPSLDGRRRFAAFEVLLNTPLITDIIRKGEVHRLKEVMTKSTELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQMGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=651619 L3V47_RS02915 WP_087757149.1 604830..605939(-) (pilU) [Aeromonas salmonicida strain ASscau0313]
ATGAATCTGGATGCCCTGTTGGCCGAATTGGTCGCACGCAAAGGCTCGGATCTGTTCATCACGGTTGGTACGCCCCCCAC
CCTCAAGATCAACGGGCATCTGTTCTCCCTCGGCAGCGAAGCGCTCGATAAGCAGAGCGCGCTGACCCTGGTCAGGGAGA
CGCTCAGCCCTGAGCATTTCGAGCGCTACATCCGCACCAAGGAGGGCAACTACGCCATCCACCGCGAGGCACTGGGACGC
TTTCGGGTGAGTGCCTTCTGGCAGCAGGAGTTGCCGGGCATGGTGGTACGCCGTATCGAGACCCGCATTCCAACCTTCGA
CGAACTGACCCTGCCGCCCATCTTGCAAGAGGTGGCCATGGCCAAACGCGGCCTGGTGCTGTTCGTCGGTGCCACCGGAA
CGGGCAAGTCCACCACTCAGGCAGCCATGATCGGCTATCGAAACCAGAATGCGGATGGCCATATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGGGAAGTGGGTATAGATACGGAATCATTCGATGT
GGCGCTGAAGAGCTCCCTGCGCCAGGCACCTGACGTTATCCTCATCGGCGAGATCCGAAGCCAGGAAACCATGGAGTTCG
CACTGCAATTTGCCGAGACCGGCCACCTTTGCCTCGCGACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAGGAGAAACACCGTCAGTTACTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
ACTGGTGCCAAGCCTGGATGGTCGCCGCCGCTTCGCGGCCTTTGAAGTGCTGCTCAACACGCCGCTCATCACGGATATCA
TCCGCAAAGGCGAGGTTCATCGTCTCAAGGAGGTGATGACCAAGTCCACCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTACCCTGTTTTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCCGGCCGTGAGCAGATGGGTGCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.239

99.729

0.591
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

39.942

92.954

0.371
  pilT Legionella pneumophila strain ERS1305867

39.942

92.954

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36