Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   SPYJRS4_RS00655 Genome accession   NZ_AP012335
Coordinates   104099..105037 (+) Length   312 a.a.
NCBI ID   WP_002986557.1    Uniprot ID   -
Organism   Streptococcus pyogenes JRS4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99099..110037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYJRS4_RS00645 (SPYJRS4_0091) rpoC 99848..103489 (+) 3642 WP_011184087.1 DNA-directed RNA polymerase subunit beta' -
  SPYJRS4_RS00650 (SPYJRS4_0092) - 103641..104006 (+) 366 WP_002986560.1 DUF1033 family protein -
  SPYJRS4_RS00655 (SPYJRS4_0093) comYA 104099..105037 (+) 939 WP_002986557.1 competence type IV pilus ATPase ComGA Machinery gene
  SPYJRS4_RS00660 (SPYJRS4_0094) comYB 104973..106007 (+) 1035 WP_011184088.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPYJRS4_RS00665 (SPYJRS4_0095) comYC 106009..106335 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  SPYJRS4_RS00670 (SPYJRS4_0096) comGD 106310..106738 (+) 429 WP_011184089.1 competence type IV pilus minor pilin ComGD -
  SPYJRS4_RS00675 (SPYJRS4_0097) comGE 106695..106979 (+) 285 WP_027968839.1 competence type IV pilus minor pilin ComGE -
  SPYJRS4_RS00680 (SPYJRS4_0098) comYF 106972..107406 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPYJRS4_RS00685 (SPYJRS4_0099) comGG 107390..107716 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  SPYJRS4_RS00690 (SPYJRS4_0100) comYH 107814..108767 (+) 954 WP_021340467.1 class I SAM-dependent methyltransferase Machinery gene
  SPYJRS4_RS00695 (SPYJRS4_0101) - 108826..110022 (+) 1197 WP_002986533.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35087.35 Da        Isoelectric Point: 8.5799

>NTDB_id=65156 SPYJRS4_RS00655 WP_002986557.1 104099..105037(+) (comYA) [Streptococcus pyogenes JRS4]
MVQVLAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGVTKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=65156 SPYJRS4_RS00655 WP_002986557.1 104099..105037(+) (comYA) [Streptococcus pyogenes JRS4]
ATGGTACAAGTATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAGGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAATTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAGATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTGTAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

61.736

99.679

0.615
  comYA Streptococcus mutans UA140

61.736

99.679

0.615
  comYA Streptococcus gordonii str. Challis substr. CH1

60.772

99.679

0.606
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.645

99.359

0.603
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.486

99.679

0.593
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.842

99.679

0.587
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

39.655

92.949

0.369

Multiple sequence alignment