Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   TBH_RS02975 Genome accession   NZ_AP012273
Coordinates   607238..608485 (-) Length   415 a.a.
NCBI ID   WP_041065303.1    Uniprot ID   A0A7U6GH26
Organism   Thiolapillus brandeum strain Hiromi     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 602238..613485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TBH_RS02945 (TBH_C0569) argJ 602499..603701 (+) 1203 WP_041065285.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  TBH_RS02950 (TBH_C0570) - 603685..604644 (+) 960 WP_041065288.1 Nudix family hydrolase -
  TBH_RS02955 (TBH_C0571) - 604641..604823 (-) 183 WP_041065291.1 DNA gyrase inhibitor YacG -
  TBH_RS02960 (TBH_C0572) zapD 604828..605595 (-) 768 WP_052469822.1 cell division protein ZapD -
  TBH_RS02965 (TBH_C0573) coaE 605740..606354 (-) 615 WP_041065297.1 dephospho-CoA kinase -
  TBH_RS02970 (TBH_C0574) - 606366..607238 (-) 873 WP_041065300.1 prepilin peptidase -
  TBH_RS02975 (TBH_C0575) pilC 607238..608485 (-) 1248 WP_041065303.1 type II secretion system F family protein Machinery gene
  TBH_RS02980 (TBH_C0576) pilB 608491..610206 (-) 1716 WP_041065306.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TBH_RS02985 (TBH_C0577) - 610523..611410 (+) 888 WP_041065309.1 glycosyltransferase family 2 protein -
  TBH_RS02990 (TBH_C0578) - 611490..612731 (+) 1242 WP_041065312.1 oligosaccharide flippase family protein -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 45469.60 Da        Isoelectric Point: 10.0483

>NTDB_id=65074 TBH_RS02975 WP_041065303.1 607238..608485(-) (pilC) [Thiolapillus brandeum strain Hiromi]
MATRTVAKKAVKKPKAEKTYEYAWTGTDRRGKKIKGKARSTNEALVRADLRRQGVTPIKVQKQITISFGKPKITTGDIAV
FSRQLATMMKAGVPLVQALDIVGKGHNNPTMQEMILSIKSDIEGGTSLTNALRKHPIQFDDLFCNLVEAGEQAGVLETLL
DRIATYKEKTESIKKKIKKALIYPAAVLSVGIIVTAIILIFVVPVFEELFESFGAELPAFTQMVVSMSRFIRDNWVFIVL
GLAAVIIIISNLWKRSPKFREIIDRGMLSAPVFGPIMHKSALARFARTTSTMFAAGVPMVEALDSVAGATGSVVYGNAVR
EMREDISTGQSLRLAMEQQRLFPHMVKQMVAIGEESGSLDEMLAKVADFYEEEVDNAVDSLSSLMEPLIMVILGTLVGGL
VVAMYLPIFKLGAAI

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=65074 TBH_RS02975 WP_041065303.1 607238..608485(-) (pilC) [Thiolapillus brandeum strain Hiromi]
ATGGCGACGAGAACAGTAGCAAAAAAAGCCGTAAAAAAACCAAAGGCGGAAAAAACCTACGAATATGCCTGGACCGGAAC
GGACAGAAGGGGCAAGAAAATCAAGGGCAAAGCGCGCTCAACCAACGAAGCCCTCGTTCGCGCAGATCTGCGCCGCCAAG
GTGTTACCCCGATCAAGGTTCAAAAGCAGATCACCATTTCCTTCGGCAAACCCAAAATCACCACGGGAGATATTGCGGTA
TTCTCCCGCCAGTTGGCGACCATGATGAAAGCGGGTGTCCCGCTGGTGCAGGCCCTGGATATCGTGGGAAAAGGCCATAA
CAATCCAACCATGCAGGAAATGATTCTATCCATCAAATCGGATATCGAGGGAGGCACATCGCTTACCAACGCCCTGCGCA
AGCACCCCATACAGTTCGATGATCTGTTTTGCAACCTGGTGGAAGCCGGTGAACAGGCGGGTGTATTGGAAACCCTTTTG
GATCGGATAGCCACTTATAAGGAAAAAACCGAATCCATCAAGAAAAAAATCAAGAAAGCACTGATTTACCCGGCAGCGGT
ACTGAGTGTCGGCATTATCGTAACCGCCATTATTCTCATATTTGTTGTTCCCGTGTTCGAAGAATTGTTCGAGAGTTTTG
GCGCCGAGCTGCCTGCATTCACCCAGATGGTTGTTAGCATGTCCCGTTTCATTCGAGACAACTGGGTCTTTATCGTTCTG
GGCCTGGCGGCAGTGATTATCATCATATCCAACCTATGGAAACGTTCTCCAAAATTCCGTGAAATTATCGACCGGGGCAT
GCTTTCAGCACCGGTTTTCGGCCCCATCATGCACAAATCCGCCCTGGCTCGATTCGCCCGAACCACATCCACCATGTTTG
CCGCCGGCGTGCCCATGGTGGAAGCACTGGATTCCGTAGCTGGCGCCACGGGTAGCGTGGTTTATGGCAACGCCGTCAGG
GAAATGCGTGAAGACATTTCAACTGGCCAATCCTTGCGCTTGGCCATGGAACAACAGCGTCTGTTTCCACACATGGTCAA
ACAGATGGTAGCCATCGGCGAAGAATCCGGCTCTCTGGATGAGATGCTGGCCAAGGTCGCCGACTTCTACGAAGAGGAGG
TAGACAACGCCGTTGACTCTTTGAGCAGTCTCATGGAACCTCTCATCATGGTTATCCTGGGTACCCTGGTCGGTGGACTG
GTCGTAGCCATGTACCTGCCTATCTTCAAGCTGGGCGCTGCCATCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(81-204)

Type II secretion system (T2SS), protein F (T2SSF)

(285-407)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U6GH26

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baumannii D1279779

55.501

98.554

0.547
  pilC Legionella pneumophila strain ERS1305867

56.313

95.422

0.537
  pilC Pseudomonas stutzeri DSM 10701

54.975

96.867

0.533
  pilC Acinetobacter baylyi ADP1

54.408

95.663

0.52
  pilG Neisseria gonorrhoeae MS11

49.482

93.012

0.46
  pilG Neisseria meningitidis 44/76-A

49.223

93.012

0.458
  pilC Vibrio campbellii strain DS40M4

42.537

96.867

0.412
  pilC Vibrio cholerae strain A1552

42.494

94.699

0.402
  pilC Thermus thermophilus HB27

38.557

96.867

0.373

Multiple sequence alignment