Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   TBH_RS02980 Genome accession   NZ_AP012273
Coordinates   608491..610206 (-) Length   571 a.a.
NCBI ID   WP_041065306.1    Uniprot ID   A0A7U6JGI8
Organism   Thiolapillus brandeum strain Hiromi     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 603491..615206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TBH_RS02950 (TBH_C0570) - 603685..604644 (+) 960 WP_041065288.1 Nudix family hydrolase -
  TBH_RS02955 (TBH_C0571) - 604641..604823 (-) 183 WP_041065291.1 DNA gyrase inhibitor YacG -
  TBH_RS02960 (TBH_C0572) zapD 604828..605595 (-) 768 WP_052469822.1 cell division protein ZapD -
  TBH_RS02965 (TBH_C0573) coaE 605740..606354 (-) 615 WP_041065297.1 dephospho-CoA kinase -
  TBH_RS02970 (TBH_C0574) - 606366..607238 (-) 873 WP_041065300.1 prepilin peptidase -
  TBH_RS02975 (TBH_C0575) pilC 607238..608485 (-) 1248 WP_041065303.1 type II secretion system F family protein Machinery gene
  TBH_RS02980 (TBH_C0576) pilB 608491..610206 (-) 1716 WP_041065306.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TBH_RS02985 (TBH_C0577) - 610523..611410 (+) 888 WP_041065309.1 glycosyltransferase family 2 protein -
  TBH_RS02990 (TBH_C0578) - 611490..612731 (+) 1242 WP_041065312.1 oligosaccharide flippase family protein -
  TBH_RS02995 (TBH_C0579) - 612826..613926 (+) 1101 WP_041065315.1 glycosyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62821.80 Da        Isoelectric Point: 4.7730

>NTDB_id=65075 TBH_RS02980 WP_041065306.1 608491..610206(-) (pilB) [Thiolapillus brandeum strain Hiromi]
MNEDSNKNLKGFSRYLVNAGLLELESALEAEKKAKDDDVPLITHVVRTGLVPSRKIALLAHQEFGLPLLDLDALSKDSLA
VNEVEPQLFNKHHVLPLVRRGNRLFLAASDPTNQEAIKEIQFSTGLAIEIILVEEEKLASALEDASKSQEAAMGAMLEEN
LENIDFGLDGEGDIDEEGQAEIDEAPVVRFVNKILLDAINSGVSDIHFEPYEKSYRIRYRQDGLLFEKASPPVNIAQRLS
SRLKVMARLNIAERRVPQDGRIKLKVSKHQAIDFRVNTCPTVYGEKVVLRILDASAATLGVDALGFEEDQKELFLKAIKK
PYGMILVTGPTGSGKTVSLYTALNILNSPEVNISTAEDPVEIQVEGINQVNINTASGLHFADALRAFLRQDPDIIMVGEI
RDLETGEIAVKAAQTGHMVLSTLHTNDAPQTLTRMANMGIPPYNIASSVNLIMAQRLGRRLCDHCKEVEDLPRTALLEEG
FSEAEVASGLTIYKAVGCDKCNNGYKGRVGIFEILPVSEGIAQIILEEGNAMQLKDQALKEGYYYDLRTAGLLKVKNGIT
SLEEINRITKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=65075 TBH_RS02980 WP_041065306.1 608491..610206(-) (pilB) [Thiolapillus brandeum strain Hiromi]
ATGAATGAAGACTCAAACAAGAATCTGAAGGGATTTTCCCGCTATCTGGTGAATGCGGGACTGCTGGAACTCGAATCGGC
ACTGGAGGCCGAAAAAAAAGCCAAGGATGACGATGTACCGCTGATTACCCACGTTGTTCGAACAGGACTGGTTCCTTCCA
GAAAAATTGCCTTGTTGGCCCACCAGGAGTTTGGCCTGCCCCTGCTGGATCTCGATGCCCTAAGCAAAGACAGCCTCGCG
GTAAACGAAGTCGAACCTCAGCTTTTTAACAAGCATCATGTCCTGCCCCTGGTCCGACGCGGCAACCGGCTGTTCCTTGC
CGCCTCCGACCCCACCAATCAGGAAGCCATAAAGGAAATCCAGTTCAGCACCGGGCTAGCCATCGAAATCATCCTGGTTG
AGGAAGAGAAGCTGGCATCCGCCCTCGAAGACGCATCCAAGTCTCAGGAAGCAGCCATGGGCGCGATGCTGGAAGAAAAC
CTGGAGAACATCGATTTTGGCCTGGATGGTGAGGGCGACATAGATGAAGAAGGCCAGGCTGAAATCGACGAGGCTCCCGT
TGTTCGATTCGTCAACAAGATATTGCTGGACGCTATCAACAGCGGAGTTTCCGACATTCATTTCGAACCCTATGAAAAAA
GCTATCGCATACGCTATCGCCAGGATGGCTTGCTGTTTGAAAAAGCCAGCCCGCCTGTGAATATCGCCCAACGCCTTTCC
TCTCGCCTCAAGGTCATGGCGCGCCTGAACATTGCCGAACGCAGGGTTCCTCAGGATGGACGTATCAAGCTCAAGGTTTC
CAAACATCAGGCCATCGATTTCCGGGTCAACACCTGCCCGACGGTCTATGGAGAAAAAGTCGTTCTGCGTATTCTCGATG
CCTCAGCCGCCACTTTGGGCGTCGATGCCCTGGGTTTCGAGGAAGACCAGAAAGAGCTCTTTTTGAAGGCTATCAAGAAG
CCTTATGGCATGATTCTGGTTACCGGCCCGACCGGCAGTGGTAAAACCGTCTCCCTGTACACCGCTCTGAACATTCTCAA
CAGTCCTGAAGTCAACATATCCACAGCGGAAGACCCTGTTGAAATCCAGGTTGAAGGAATCAACCAGGTCAACATCAATA
CCGCCAGCGGATTGCATTTTGCCGATGCGCTGCGCGCTTTTCTGCGTCAGGATCCGGACATCATCATGGTGGGGGAGATA
CGTGACCTGGAAACCGGAGAAATCGCCGTCAAAGCCGCTCAGACAGGCCATATGGTTCTGTCCACCCTGCATACCAATGA
TGCCCCTCAAACACTGACGCGGATGGCCAACATGGGTATTCCCCCATACAACATCGCTTCTTCGGTCAATCTGATTATGG
CCCAGCGACTTGGGCGACGCCTGTGCGATCACTGCAAGGAAGTCGAAGATCTGCCTCGCACCGCCCTCCTGGAAGAGGGT
TTCTCGGAAGCAGAAGTCGCCTCAGGCCTTACCATCTACAAAGCAGTTGGTTGTGACAAGTGCAATAATGGCTATAAGGG
GCGGGTAGGTATCTTCGAAATTCTTCCGGTTTCCGAAGGCATAGCTCAGATCATTCTGGAAGAAGGAAATGCTATGCAGC
TCAAGGATCAGGCCTTGAAAGAAGGCTATTATTATGATCTGCGGACCGCGGGCCTGTTGAAAGTGAAGAACGGCATCACC
AGTCTGGAAGAAATCAACCGAATCACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U6JGI8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.833

100

0.541
  pilB Legionella pneumophila strain ERS1305867

53.968

99.299

0.536
  pilB Acinetobacter baylyi ADP1

51.295

100

0.52
  pilF Neisseria gonorrhoeae MS11

49.47

99.124

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

45.922

98.774

0.454
  pilB Vibrio cholerae strain A1552

50

89.667

0.448
  pilB Vibrio campbellii strain DS40M4

44.797

99.299

0.445
  pilF Thermus thermophilus HB27

38.272

99.299

0.38

Multiple sequence alignment