Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L1S45_RS03880 Genome accession   NZ_CP091091
Coordinates   787117..788226 (-) Length   369 a.a.
NCBI ID   WP_017780455.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain b2-100     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 782117..793226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1S45_RS03860 (L1S45_03860) brnQ 782575..783882 (-) 1308 WP_005305030.1 branched-chain amino acid transport system II carrier protein -
  L1S45_RS03865 (L1S45_03865) - 784073..784783 (-) 711 WP_252603848.1 methyltransferase -
  L1S45_RS03870 (L1S45_03870) srmB 784912..786135 (+) 1224 WP_252603851.1 ATP-dependent RNA helicase SrmB -
  L1S45_RS03875 (L1S45_03875) yaaA 786312..787085 (-) 774 WP_123246604.1 peroxide stress protein YaaA -
  L1S45_RS03880 (L1S45_03880) pilU 787117..788226 (-) 1110 WP_017780455.1 type IVa pilus ATPase TapU Machinery gene
  L1S45_RS03885 (L1S45_03885) pilT 788255..789289 (-) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  L1S45_RS03890 (L1S45_03890) - 789329..790030 (+) 702 WP_099359980.1 YggS family pyridoxal phosphate-dependent enzyme -
  L1S45_RS03895 (L1S45_03895) proC 790091..790915 (+) 825 WP_252603853.1 pyrroline-5-carboxylate reductase -
  L1S45_RS03900 (L1S45_03900) - 790956..791507 (+) 552 WP_005305000.1 YggT family protein -
  L1S45_RS03905 (L1S45_03905) yggU 791507..791806 (+) 300 WP_005304998.1 DUF167 family protein YggU -
  L1S45_RS03910 (L1S45_03910) - 791825..792244 (+) 420 WP_005304995.1 DUF4426 domain-containing protein -
  L1S45_RS03915 (L1S45_03915) - 792308..792619 (-) 312 WP_005304992.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41097.22 Da        Isoelectric Point: 6.5672

>NTDB_id=647597 L1S45_RS03880 WP_017780455.1 787117..788226(-) (pilU) [Aeromonas dhakensis strain b2-100]
MNLDALLTELVSRKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSPEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSPDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=647597 L1S45_RS03880 WP_017780455.1 787117..788226(-) (pilU) [Aeromonas dhakensis strain b2-100]
ATGAATCTGGATGCCCTGCTGACCGAACTGGTCTCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCATCTGCAGTCGCTGGCAGAAGCGCCCCTCGACAAGGCAAGGGCACTGGCGCTGGTGAAGGAGA
CCCTGAGCCCGGAGCACTTCGAGCGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCACTGCCCCCCATATTGCAGGAGATAGCCCTGGCCAAGCGCGGACTGGTGCTGTTCGTCGGCGCCACCGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCATCGTTCGATGT
GGCACTGAAGAGCTCGCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGTCACCTCTGCCTCGCCACCCTGCATGCCAACAACGCCAACCAGGCTCTGGATCGCATC
CTGCATCTGGTGCCCCAGGAGAAACACCGTCAGTTCCTGTTCGATCTCTCCTTCAATCTGCGGGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCCGGATGGACGGCGTCGTTTTGCCGCCTTCGAGGTGTTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAGATGCACCGCCTCAAGGAGGTGATGACCAAGTCCACCGAACTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGCCGAGAGCAGCTGGGTTCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.87

99.729

0.607
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363