Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   L0994_RS01220 Genome accession   NZ_CP090847
Coordinates   269520..271211 (+) Length   563 a.a.
NCBI ID   WP_016787694.1    Uniprot ID   A0AAN0LTR6
Organism   Vibrio cyclitrophicus strain WXL032     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 264520..276211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0994_RS01200 (L0994_01195) pilM 266710..267735 (+) 1026 WP_016787692.1 type IV pilus assembly protein PilM Machinery gene
  L0994_RS01205 (L0994_01200) pilN 267719..268333 (+) 615 WP_010435288.1 PilN domain-containing protein Machinery gene
  L0994_RS01210 (L0994_01205) pilO 268326..268955 (+) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  L0994_RS01215 (L0994_01210) pilP 268942..269460 (+) 519 WP_016787693.1 pilus assembly protein PilP Machinery gene
  L0994_RS01220 (L0994_01215) pilQ 269520..271211 (+) 1692 WP_016787694.1 type IV pilus secretin PilQ Machinery gene
  L0994_RS01225 (L0994_01220) aroK 271388..271906 (+) 519 WP_009848907.1 shikimate kinase AroK -
  L0994_RS01230 (L0994_01225) aroB 271931..273019 (+) 1089 WP_010435273.1 3-dehydroquinate synthase -
  L0994_RS01235 (L0994_01230) - 273100..274617 (+) 1518 WP_016787695.1 SPOR domain-containing protein -
  L0994_RS01240 (L0994_01235) - 274687..275532 (+) 846 WP_010435266.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 61435.94 Da        Isoelectric Point: 4.3414

>NTDB_id=645202 L0994_RS01220 WP_016787694.1 269520..271211(+) (pilQ) [Vibrio cyclitrophicus strain WXL032]
MNRGLQGIVLSVVLFFTVMSHAETSNKLENIDFRVNKDKEAVIIIELATTTAVVDVQRVQEGLSIELINTEVDDDKLYLL
DVKDFATLVEGIEVFRELPSTRLLANISNDYQYEYNLKGRFIEVVVSQPKIDEAAIEKSILEKEGKLISINFQDIPVRNV
LQLIADYNDFNLVVSDSVAGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKAELDLREQQALEKSRLEEELGE
LKSEIIKINFAKATDIADMIGGEGAVSMLSDRGSITIDERTNSMLIRELEENIAVIRGIIESLDIPVKQVQIEARIVTVT
EGNLDELGVRWGVSSNNGSFTVGGSIEGNHPSQITPYDDNGGNSAIDDYLNVNLGATSPNASSIAFQVAKLGSDTLLDLE
LSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPG
QVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPVLGALFRRSYENVGKSELLIFVTPKV
VIQ

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=645202 L0994_RS01220 WP_016787694.1 269520..271211(+) (pilQ) [Vibrio cyclitrophicus strain WXL032]
ATGAACAGAGGGCTGCAAGGCATTGTGTTGTCGGTGGTGTTGTTTTTTACGGTGATGAGTCATGCAGAGACCTCCAACAA
GCTGGAGAATATTGATTTCAGGGTCAACAAGGACAAAGAAGCGGTCATCATTATTGAACTGGCAACGACTACTGCGGTTG
TCGATGTTCAGCGAGTGCAAGAAGGGTTGAGTATTGAACTGATCAATACTGAGGTTGACGACGATAAACTGTACCTACTG
GACGTAAAGGACTTTGCGACTTTAGTTGAGGGGATTGAAGTCTTTAGGGAGTTGCCGAGCACTCGGCTGTTGGCAAACAT
CTCCAATGACTACCAATATGAGTACAACCTCAAAGGACGTTTTATTGAGGTTGTTGTGAGTCAGCCTAAGATCGATGAAG
CTGCGATTGAGAAAAGTATCTTAGAAAAAGAGGGCAAACTGATATCGATTAACTTCCAAGATATCCCGGTACGAAATGTC
TTGCAGCTGATTGCCGATTACAATGATTTCAATCTAGTCGTATCTGATTCGGTGGCAGGTAACCTAACTTTACGTTTAGA
TGGCGTACCTTGGCAGCAAGTTCTCGATATTATCTTGCAAGTTAAAGGCTTAGATAAACGTGTTGATGGCAACGTTATCT
TGGTCGCACCGAAAGCGGAACTCGATCTTCGAGAGCAGCAAGCGCTCGAGAAATCGCGTTTAGAAGAGGAGTTGGGTGAA
CTCAAATCTGAAATCATCAAAATCAACTTTGCTAAAGCGACCGACATTGCCGACATGATTGGCGGTGAAGGTGCGGTGAG
TATGTTGTCTGATCGAGGTTCAATTACCATTGATGAACGAACCAACTCAATGCTCATTCGTGAGTTGGAAGAAAACATTG
CGGTGATCCGAGGCATCATTGAATCGCTAGATATTCCGGTGAAGCAAGTTCAGATAGAGGCGCGCATCGTAACTGTCACC
GAGGGTAACCTGGATGAGCTTGGTGTGCGCTGGGGCGTTTCCTCAAACAACGGAAGCTTCACCGTTGGCGGTTCAATTGA
AGGCAACCACCCATCGCAAATTACGCCGTATGACGATAATGGTGGGAATAGTGCGATTGATGATTATCTCAACGTCAATT
TAGGGGCAACATCGCCGAACGCATCGAGTATTGCGTTTCAGGTTGCTAAGTTGGGTTCGGATACGCTGCTCGATCTTGAA
TTATCAGCGCTACAACAAGAATCTAAAGCCGAAATAATTTCGAGCCCACGCTTAATCACCACCAATAAAAAACCGGCTTA
TATTGAGCAAGGTACGGAAATTCCTTATTTGGAGTCCTCTTCAAGTGGTGCAACGTCGGTCGCATTTAAGAAAGCGGTAT
TAAGTTTGAAGGTGACACCGCAGATCACGCCAGATAACCGCTTAGTGTTGGACTTGAGTGTGACTCAAGATAGACCTGGG
CAGGTAGTGAAAACAGGAACGGGCGAAGCAGTGGCCATTGATACTCAACGAATAGGAACGCAAGTGCTTGTTAATAATGG
TGAAACAGTCGTACTTGGCGGGATTTTTCAACATAGTGTCAGCAGTACTGTCGACAAAGTTCCGCTGTTGGGCGACCTGC
CAGTTTTAGGCGCATTGTTCCGTCGCAGCTATGAAAATGTAGGCAAAAGTGAACTGCTTATTTTTGTTACACCTAAAGTT
GTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(244-309)

Bacterial type II and III secretion system protein (Secretin)

(403-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

81.101

96.803

0.785
  pilQ Vibrio cholerae strain A1552

79.963

96.625

0.773
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

79.963

96.625

0.773
  pilQ Legionella pneumophila strain ERS1305867

42.857

96.98

0.416
  pilQ Legionella pneumophila strain Lp02

42.857

96.98

0.416
  pilQ Pseudomonas aeruginosa PAK

40.248

100

0.403
  pilQ Acinetobacter baumannii strain A118

37.167

100

0.396
  pilQ Acinetobacter baumannii D1279779

37.167

100

0.396
  comQ Acinetobacter baylyi ADP1

39.046

100

0.393