Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   L0993_RS13360 Genome accession   NZ_CP090845
Coordinates   2970509..2972200 (-) Length   563 a.a.
NCBI ID   WP_186704436.1    Uniprot ID   -
Organism   Vibrio kanaloae strain WXL161     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2965509..2977200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0993_RS13340 (L0993_13325) - 2966173..2967033 (-) 861 WP_046224649.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  L0993_RS13345 (L0993_13330) - 2967103..2968620 (-) 1518 WP_137008137.1 SPOR domain-containing protein -
  L0993_RS13350 (L0993_13335) aroB 2968700..2969788 (-) 1089 WP_146441603.1 3-dehydroquinate synthase -
  L0993_RS13355 (L0993_13340) aroK 2969814..2970332 (-) 519 WP_017061125.1 shikimate kinase AroK -
  L0993_RS13360 (L0993_13345) pilQ 2970509..2972200 (-) 1692 WP_186704436.1 type IV pilus secretin PilQ Machinery gene
  L0993_RS13365 (L0993_13350) pilP 2972260..2972778 (-) 519 WP_077680947.1 pilus assembly protein PilP Machinery gene
  L0993_RS13370 (L0993_13355) pilO 2972765..2973376 (-) 612 WP_146441597.1 type 4a pilus biogenesis protein PilO Machinery gene
  L0993_RS13375 (L0993_13360) pilN 2973369..2973968 (-) 600 WP_146441594.1 PilN domain-containing protein Machinery gene
  L0993_RS13380 (L0993_13365) pilM 2973952..2974977 (-) 1026 WP_373937899.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 61318.90 Da        Isoelectric Point: 4.4300

>NTDB_id=645182 L0993_RS13360 WP_186704436.1 2970509..2972200(-) (pilQ) [Vibrio kanaloae strain WXL161]
MNKGLQGLIVSVMLIFSVLSHAEDLPNKLENIDFRVNKNKDAVLIIELATSTVVVDVQRAQEGLSIELINTHVDDNKLQL
LDVKDFATLVEGVEVYKETPSTRLLATISKDYQYEYNLKGRFIEVVISKPAIEEAVTEKSILEKEGKLISINFQDIPVRN
VLQLIADYNDFNLVVSDSVTGSLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREQQALEKSRLEEELG
ELKSEIIKIHFAKATDIADMIGGDGTVSMLSDRGSITIDERTNSLLIRELEENIAVIRSIIESLDVPVKQVQIEARIVTV
TEGNLDELGVRWGASSTNGSFTVGGSIEGNYPSIAPYDSNGGNSVIDEYLNVNLGATSSNASSIAFQVAKLGSDTLLDLE
LSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPG
QVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPVLGALFRRSYENVGKSELLIFVTPKV
VIQ

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=645182 L0993_RS13360 WP_186704436.1 2970509..2972200(-) (pilQ) [Vibrio kanaloae strain WXL161]
ATGAACAAAGGGTTACAAGGCTTGATTGTGTCTGTGATGTTGATCTTTAGTGTGCTCAGTCACGCAGAGGACTTACCTAA
TAAGTTAGAAAATATTGATTTTAGGGTGAACAAAAATAAAGACGCCGTCCTAATTATCGAGCTCGCAACCAGCACGGTTG
TGGTGGATGTTCAACGTGCACAAGAGGGGCTAAGCATAGAGCTGATCAATACTCATGTTGATGACAACAAACTTCAGCTT
CTTGATGTGAAAGATTTCGCTACCTTGGTTGAAGGCGTGGAAGTGTATAAAGAGACCCCAAGCACTCGGTTGTTAGCGAC
AATCAGCAAGGATTACCAATACGAATACAATCTCAAAGGCCGTTTTATTGAGGTGGTGATCAGTAAACCTGCTATAGAAG
AAGCGGTAACAGAGAAAAGTATACTCGAGAAAGAGGGTAAGCTGATATCGATTAACTTCCAAGATATTCCAGTGCGAAAT
GTCCTGCAGCTTATCGCTGACTATAACGACTTTAACTTAGTCGTGTCTGATTCAGTAACCGGAAGCCTAACTTTACGTTT
AGATGGTGTGCCTTGGCAGCAAGTTCTAGATATCATCTTACAGGTCAAAGGGTTAGATAAGCGGGTAGATGGCAATGTCA
TCTTGGTGGCGCCAAAAGATGAACTCGACCTTCGAGAGCAGCAAGCGTTAGAGAAGTCGCGTTTAGAAGAGGAATTGGGT
GAGCTTAAGTCGGAAATCATTAAGATTCACTTTGCTAAAGCGACCGACATTGCCGACATGATTGGTGGTGATGGGACGGT
GAGCATGTTGTCTGATCGGGGCTCGATTACTATTGATGAGCGCACTAATTCATTGCTTATTCGTGAACTGGAGGAAAACA
TTGCGGTGATACGAAGCATTATTGAATCTTTAGATGTCCCTGTGAAGCAAGTTCAGATAGAGGCGCGCATCGTGACTGTG
ACTGAGGGGAACCTTGATGAGCTAGGAGTACGTTGGGGAGCTTCTTCAACAAACGGAAGCTTCACGGTTGGTGGTTCTAT
CGAAGGCAACTACCCGTCTATTGCACCTTACGATAGTAATGGAGGGAATAGCGTCATTGATGAGTACCTCAACGTTAATT
TAGGTGCGACATCTTCAAATGCTTCGAGTATCGCATTTCAGGTTGCTAAACTGGGCTCTGATACTCTGCTCGACCTTGAG
TTATCAGCGTTACAACAAGAGTCAAAAGCTGAAATTATTTCGAGTCCACGTTTGATTACTACCAATAAAAAGCCCGCTTA
TATTGAGCAAGGTACTGAAATTCCTTATTTAGAGTCATCTTCAAGTGGTGCCACGTCAGTCGCATTTAAGAAAGCGGTAT
TGAGCCTTAAGGTGACGCCGCAGATCACGCCAGACAATCGTTTGGTGTTGGATTTGAGCGTAACTCAAGATAGGCCCGGA
CAAGTGGTAAAAACCGGAACTGGGGAAGCTGTTGCAATTGATACCCAGCGCATTGGTACGCAAGTGCTTGTTAATAATGG
TGAAACGGTTGTTCTTGGTGGGATATTTCAGCACAGTGTGAGTAGCACCGTCGATAAAGTTCCTCTATTGGGAGACCTGC
CTGTATTAGGGGCTTTGTTCCGCAGAAGCTACGAAAATGTAGGGAAAAGTGAGTTGTTGATCTTTGTTACACCGAAAGTT
GTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(245-310)

Secretin and TonB N terminus short domain (STN)

(171-219)

Bacterial type II and III secretion system protein (Secretin)

(403-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

79.006

96.448

0.762
  pilQ Vibrio cholerae strain A1552

78.571

96.98

0.762
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

78.571

96.98

0.762
  pilQ Legionella pneumophila strain Lp02

42.308

96.98

0.41
  pilQ Legionella pneumophila strain ERS1305867

42.308

96.98

0.41
  pilQ Pseudomonas aeruginosa PAK

40.727

97.691

0.398
  comQ Acinetobacter baylyi ADP1

39.107

99.467

0.389
  pilQ Acinetobacter baumannii D1279779

37.197

100

0.382
  pilQ Acinetobacter baumannii strain A118

37.197

100

0.382