Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   L0993_RS13380 Genome accession   NZ_CP090845
Coordinates   2973952..2974977 (-) Length   341 a.a.
NCBI ID   WP_373937899.1    Uniprot ID   -
Organism   Vibrio kanaloae strain WXL161     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2968952..2979977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0993_RS13355 (L0993_13340) aroK 2969814..2970332 (-) 519 WP_017061125.1 shikimate kinase AroK -
  L0993_RS13360 (L0993_13345) pilQ 2970509..2972200 (-) 1692 WP_186704436.1 type IV pilus secretin PilQ Machinery gene
  L0993_RS13365 (L0993_13350) pilP 2972260..2972778 (-) 519 WP_077680947.1 pilus assembly protein PilP Machinery gene
  L0993_RS13370 (L0993_13355) pilO 2972765..2973376 (-) 612 WP_146441597.1 type 4a pilus biogenesis protein PilO Machinery gene
  L0993_RS13375 (L0993_13360) pilN 2973369..2973968 (-) 600 WP_146441594.1 PilN domain-containing protein Machinery gene
  L0993_RS13380 (L0993_13365) pilM 2973952..2974977 (-) 1026 WP_373937899.1 type IV pilus assembly protein PilM Machinery gene
  L0993_RS13385 (L0993_13370) - 2975129..2977690 (+) 2562 WP_373937901.1 penicillin-binding protein 1A -
  L0993_RS13390 (L0993_13375) oxyR 2977790..2978680 (-) 891 WP_046224655.1 DNA-binding transcriptional regulator OxyR -
  L0993_RS13395 (L0993_13380) - 2978900..2979628 (+) 729 WP_373937902.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38003.59 Da        Isoelectric Point: 7.2208

>NTDB_id=645186 L0993_RS13380 WP_373937899.1 2973952..2974977(-) (pilM) [Vibrio kanaloae strain WXL161]
MGSSLITGIDINHHSIKAVVLKPVGELYAIVGYKELPISDDIFTANHTLEYQKTVKKLKELKKGLPFGSRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRANALSKAGFKP
IVVDTQAHGLLNIWQLASYLSPEKKNWLLVDVGIDQTSLGVIPQNSAPFYKDIAFGTQELLGTGSPSDMEGVFGTTQDTH
RFIVNLIEKLKRQLQLYSSVNVLQPISGIWLMGEGASIPMVVEELERHFQMSCESLNPMSLFENKVAQKRRSSLNCQHFG
IAAGMAMRGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=645186 L0993_RS13380 WP_373937899.1 2973952..2974977(-) (pilM) [Vibrio kanaloae strain WXL161]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGTATCAAAGCTGTGGTACTAAAACCCGTTGGGGAGTT
GTATGCCATCGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAAGAAAGGACTGCCTTTTGGAAGTCGTAACGTCGCTATTTCAGTACCAGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGATAGAGAGAAAGAGTTTTCTATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAAGAGCTGAGTTTAGATTTTGTAAAGCTCGAAGACAAACGGTTTGGTAAAGGATCGACAA
GTAGTTATCAGGTGTACGCGACTCGCAAGGAAGTTGTGGATAGTCGAGCCAATGCATTAAGCAAAGCCGGGTTTAAACCT
ATCGTCGTTGATACCCAAGCACATGGACTTCTCAATATTTGGCAATTGGCTTCGTATTTGTCCCCAGAAAAGAAGAATTG
GCTGCTGGTGGACGTTGGAATCGACCAAACCTCGTTAGGGGTTATCCCGCAGAATTCTGCGCCTTTTTATAAAGACATCG
CCTTTGGTACTCAAGAGCTGTTAGGCACAGGCAGTCCAAGTGATATGGAAGGCGTGTTCGGCACCACTCAGGATACACAT
CGGTTTATTGTTAATCTTATTGAAAAGCTCAAGCGTCAACTGCAACTCTATTCGTCGGTAAATGTGCTTCAACCTATCTC
AGGGATTTGGCTAATGGGCGAAGGAGCGAGCATACCAATGGTCGTTGAGGAGTTAGAACGTCATTTTCAGATGAGTTGTG
AGTCACTGAACCCTATGTCTTTGTTTGAGAATAAGGTGGCCCAGAAACGCCGATCGTCACTGAATTGTCAGCATTTTGGT
ATTGCGGCGGGGATGGCAATGCGTGGTCTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(242-329)

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

53.392

99.413

0.531
  pilM Vibrio campbellii strain DS40M4

47.076

100

0.472