Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LZI70_RS09630 Genome accession   NZ_CP090614
Coordinates   2118691..2120376 (-) Length   561 a.a.
NCBI ID   WP_255229934.1    Uniprot ID   -
Organism   Vibrio pelagius strain WXL662     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2113691..2125376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZI70_RS09600 (LZI70_09585) rplS 2114375..2114728 (+) 354 WP_032549758.1 50S ribosomal protein L19 -
  LZI70_RS09605 (LZI70_09590) yacG 2114812..2115009 (-) 198 WP_255229929.1 DNA gyrase inhibitor YacG -
  LZI70_RS09610 (LZI70_09595) zapD 2115097..2115837 (-) 741 WP_255229930.1 cell division protein ZapD -
  LZI70_RS09615 (LZI70_09600) coaE 2115870..2116478 (-) 609 WP_255229931.1 dephospho-CoA kinase -
  LZI70_RS09620 (LZI70_09605) pilD 2116483..2117352 (-) 870 WP_255229932.1 prepilin peptidase Machinery gene
  LZI70_RS09625 (LZI70_09610) pilC 2117415..2118644 (-) 1230 WP_255229933.1 type II secretion system F family protein Machinery gene
  LZI70_RS09630 (LZI70_09615) pilB 2118691..2120376 (-) 1686 WP_255229934.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LZI70_RS09635 (LZI70_09620) pilA 2120376..2120831 (-) 456 WP_255229935.1 pilin Machinery gene
  LZI70_RS09640 (LZI70_09625) nadC 2121084..2121974 (-) 891 WP_255229936.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LZI70_RS09645 (LZI70_09630) ampD 2122121..2122669 (+) 549 WP_255229937.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LZI70_RS09650 (LZI70_09635) pdhR 2123117..2123884 (+) 768 WP_255229938.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62375.21 Da        Isoelectric Point: 5.5013

>NTDB_id=644008 LZI70_RS09630 WP_255229934.1 2118691..2120376(-) (pilB) [Vibrio pelagius strain WXL662]
MLTNLPTVLRQANLLSLAQEQVVVEHLQASGGSTPEALITHGLFSADSLAEQTAHLFGLPLISLTDYDYEPLCEQLGLRE
LITQHRVIPIAKSASTLTLASADPTDLYAEDDFRFATGLQIDSVIANYRTLESAIRRLYGKAITNGDSNRKEITQDELAH
LVEVSDDEVSTVEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEYYRVRLRCDGILIETQQPAAHLSRRLSARLKI
IAKLDIAERRLPQDGRIKLRLNQEIAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDTLGYSEQQKLLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTLERNISTAEDPVEINLCGVNQVQVQPKIGFGFAEALRSFLRQDPDIVMVGEIRDIET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCPHCKIKDDDQTIYHRHAIDANHT
FFKANPNGCNECNQGYAGRVGIYEVMPFTDKLKASLISRPNAIEIETLAKQEGMQTLRDSGLEKLLLGITSYQELQRVLY
I

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=644008 LZI70_RS09630 WP_255229934.1 2118691..2120376(-) (pilB) [Vibrio pelagius strain WXL662]
ATGCTAACCAACCTACCCACCGTTCTGCGTCAGGCTAACTTACTTAGCCTGGCTCAAGAACAAGTCGTAGTGGAACACTT
ACAAGCTTCTGGTGGCTCAACACCGGAAGCTTTAATCACACATGGCTTGTTCAGTGCCGATAGCCTTGCCGAACAGACCG
CACACCTCTTTGGCTTACCGTTAATATCACTCACTGATTACGACTATGAACCTCTGTGTGAACAACTTGGGCTGCGCGAA
CTCATCACTCAACACCGAGTCATACCGATCGCTAAATCAGCATCTACGCTTACCTTGGCTTCTGCTGACCCAACCGACCT
GTATGCTGAAGATGATTTTCGTTTTGCCACTGGACTGCAAATTGACTCAGTAATTGCAAACTATCGTACTCTTGAAAGCG
CAATTCGGCGACTTTATGGCAAAGCAATTACCAATGGTGATAGCAATCGCAAAGAGATAACCCAAGACGAACTCGCCCAT
TTGGTGGAGGTTTCTGATGATGAAGTCAGTACCGTCGAAGATCTTAGCCAAGATGACTCGCCCGTCAGCCGCTTCATCAA
TCAAATCCTAGTCGATGCAGTGCGTAAAGGCGCATCAGATATCCATTTCGAACCTTATGAGGAATATTACCGAGTCCGCC
TACGTTGCGATGGCATCTTAATCGAAACCCAACAACCGGCAGCACATCTGAGTCGGAGACTATCAGCACGTTTAAAGATC
ATTGCTAAGCTTGATATTGCGGAGCGCCGTTTACCTCAAGATGGTCGAATTAAGCTGAGGCTTAATCAAGAGATCGCCAT
CGATATGCGTGTCTCTACTCTGCCGACACTTTGGGGAGAGAAGATTGTATTGCGTCTGCTCGATAGCAGTGCAGCCAACC
TTGATATTGACACTCTTGGCTACAGTGAACAGCAGAAATTACTTTATCTCAACGCGCTAAAACGTCCACAAGGCATGATT
CTTATGACGGGCCCAACCGGCAGCGGTAAAACCGTCTCTCTTTACACAGGGCTTCGCATCCTCAACACCTTAGAAAGAAA
CATCTCTACCGCTGAAGATCCTGTAGAGATTAACCTTTGTGGCGTCAATCAAGTCCAGGTTCAACCCAAAATAGGCTTTG
GCTTTGCTGAAGCGCTGCGATCTTTTCTGCGCCAAGATCCAGATATCGTGATGGTCGGTGAAATACGCGATATTGAAACC
GCTGAAATTGCGATAAAAGCCTCCCAAACCGGCCACTTGGTGCTCTCGACACTGCACACTAACTCAGCTGCAGAAACAGT
CACACGCCTTGCACATATGGGGATTGAACCTTTTAACTTGGCTTCATCATTGAGCCTCATCATTGCTCAACGTCTCGCAA
GGCGACTATGCCCACATTGCAAAATTAAAGACGATGACCAAACCATTTATCACCGTCATGCCATTGATGCTAACCACACG
TTTTTTAAAGCGAATCCGAACGGCTGCAATGAGTGCAATCAAGGTTATGCCGGCCGTGTGGGTATCTATGAAGTGATGCC
GTTTACTGACAAGCTCAAAGCCAGTCTCATCTCCCGCCCAAACGCGATCGAGATAGAAACTCTCGCTAAACAAGAAGGTA
TGCAGACTCTCCGAGACTCTGGTTTAGAGAAGCTGTTATTGGGTATCACAAGCTACCAAGAGCTGCAGCGTGTCCTTTAT
ATCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

71.886

100

0.72
  pilB Vibrio parahaemolyticus RIMD 2210633

69.573

100

0.697
  pilB Vibrio campbellii strain DS40M4

68.984

100

0.69
  pilB Acinetobacter baumannii D1279779

49.273

98.039

0.483
  pilB Legionella pneumophila strain ERS1305867

49.716

94.296

0.469
  pilB Acinetobacter baylyi ADP1

48.864

94.118

0.46
  pilF Neisseria gonorrhoeae MS11

42.553

100

0.428
  pilF Thermus thermophilus HB27

38.309

99.109

0.38
  pilB/pilB1 Synechocystis sp. PCC 6803

37.03

99.643

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.899

95.009

0.36