Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IHC91_RS01945 Genome accession   NZ_CP090489
Coordinates   391533..393224 (-) Length   563 a.a.
NCBI ID   WP_237029686.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain AS-15-3942-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 386533..398224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHC91_RS01915 (IHC91_01915) mutT 387037..387459 (-) 423 WP_068968360.1 8-oxo-dGTP diphosphatase MutT -
  IHC91_RS01920 (IHC91_01920) yacG 387554..387763 (-) 210 WP_005297592.1 DNA gyrase inhibitor YacG -
  IHC91_RS01925 (IHC91_01925) zapD 387772..388509 (-) 738 WP_106261927.1 cell division protein ZapD -
  IHC91_RS01930 (IHC91_01930) coaE 388577..389179 (-) 603 WP_138222159.1 dephospho-CoA kinase -
  IHC91_RS01935 (IHC91_01935) pilD 389181..390059 (-) 879 WP_068945537.1 prepilin peptidase Machinery gene
  IHC91_RS01940 (IHC91_01940) pilC 390241..391467 (-) 1227 WP_106338472.1 type II secretion system F family protein Machinery gene
  IHC91_RS01945 (IHC91_01945) pilB 391533..393224 (-) 1692 WP_237029686.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IHC91_RS01950 (IHC91_01950) - 393225..393719 (-) 495 WP_237029687.1 pilin -
  IHC91_RS01955 (IHC91_01955) ampD 394238..394816 (+) 579 WP_138222126.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IHC91_RS01960 (IHC91_01960) pdhR 395176..395949 (+) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62525.17 Da        Isoelectric Point: 5.4754

>NTDB_id=642829 IHC91_RS01945 WP_237029686.1 391533..393224(-) (pilB) [Photobacterium damselae subsp. damselae strain AS-15-3942-8]
MHTTLASILHQADLISSVQQQQVEDSVSMEGITVPSALIHLGIFSDRELAQHLSHIFGLTLVSVHDYDFEACCKAVYQRD
LVLRHRALPLCQSANTLFLGVSDPCNIDGQDEFRFATGRNIEPLLLENKQLESAIRRIYGRELGETTNMASRDVSDDDLN
NLVDIGDEERDQATDLGQENEPVTRYINQVLLDAVRKKASDIHFEPYETSYRIRFRCDGILHQHATPPAHLSRRLSTRLK
VMSRLNIAERRQPQDGRIKLKLTANLAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDILGYNQQQKAAYLNALQQPQGM
ILMTGPTGSGKTVSLYTGLSILNTEERNISTAEDPVEINLPGINQVHINNQAGLGFAEALRSFLRQDPDVVMVGEIRDLE
TASIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIEDFNLASSLTLIIAQRLGRRLCPHCKQVDEIDGFMQDKLRIPAG
TTLYKANPEGCDDCNKGYLGRVGIYEVMPFSRRLAQALVDGATTLELENIAVEEGMLTLQQSGIEKLCLGITSLKELQRV
LHF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=642829 IHC91_RS01945 WP_237029686.1 391533..393224(-) (pilB) [Photobacterium damselae subsp. damselae strain AS-15-3942-8]
ATGCACACCACTCTTGCCTCGATTCTGCACCAGGCTGATCTGATCAGCTCGGTACAGCAACAACAAGTCGAAGACAGTGT
CAGTATGGAGGGGATCACGGTTCCCTCCGCACTGATCCATCTGGGCATTTTTAGCGATCGCGAACTGGCCCAACACCTTA
GCCACATATTTGGTCTCACCCTCGTTTCAGTCCATGACTACGATTTTGAAGCTTGCTGTAAAGCGGTCTATCAACGCGAT
TTAGTATTACGTCATCGTGCGCTACCGTTATGCCAAAGCGCCAATACGTTATTTTTAGGGGTCAGCGATCCATGCAATAT
CGACGGGCAAGATGAGTTCCGTTTTGCCACTGGCCGCAATATTGAACCGTTACTGCTAGAAAACAAACAGCTCGAATCAG
CTATCCGTCGTATTTATGGTCGTGAGTTAGGCGAAACAACCAATATGGCCAGTCGAGATGTCAGCGACGATGATCTGAAT
AACTTAGTCGATATTGGTGATGAAGAGCGGGATCAAGCGACAGATCTAGGTCAAGAAAACGAGCCTGTTACTCGTTACAT
CAACCAAGTACTGCTCGATGCGGTACGTAAAAAAGCCTCCGATATCCATTTTGAACCTTATGAGACCAGTTATCGGATCC
GCTTTCGTTGTGATGGGATCTTGCACCAACACGCAACACCACCAGCCCATCTTTCTCGCCGACTCTCAACCCGACTCAAA
GTGATGTCGCGCCTAAATATTGCCGAACGACGCCAACCACAAGATGGACGTATAAAACTCAAGCTCACAGCCAATTTAGC
CGTAGATCTGCGGGTTTCTACCCTGCCCACTTTATGGGGGGAAAAAGTGGTGCTGCGTATTTTAGACAGCTCCAGTGCCA
ACTTAGACATTGATATTTTAGGTTACAACCAGCAACAAAAAGCCGCTTATCTTAATGCTCTGCAACAACCGCAAGGAATG
ATCTTAATGACAGGGCCTACGGGTAGCGGCAAAACCGTTTCGCTTTATACCGGCCTTAGCATTTTAAATACCGAAGAGCG
CAATATTTCTACCGCTGAAGATCCGGTAGAGATCAACTTACCCGGCATTAATCAGGTTCACATTAACAATCAAGCAGGAC
TGGGATTTGCTGAAGCGCTGCGCTCATTTTTACGTCAAGACCCTGATGTGGTGATGGTGGGGGAAATCCGCGATCTGGAA
ACCGCCTCTATTGCCGTTAAAGCAGCGCAAACTGGACATTTAGTACTATCGACCTTGCACACCAATTCTGCCGCCGAAAC
CATTACCCGTTTAACCAATATGGGTATTGAAGACTTTAACCTAGCCTCATCACTGACGCTGATTATTGCTCAGCGTTTAG
GTCGCCGGCTCTGCCCTCACTGTAAACAAGTAGATGAGATTGACGGCTTTATGCAAGACAAACTGCGCATTCCAGCTGGC
ACTACCTTGTATAAAGCCAATCCAGAAGGGTGTGATGACTGCAATAAAGGCTATTTAGGTCGTGTCGGTATCTATGAAGT
GATGCCATTTAGTCGTCGTCTGGCTCAAGCTCTGGTTGATGGCGCAACTACCTTAGAGCTGGAAAATATCGCAGTGGAAG
AAGGGATGCTAACGCTACAACAATCGGGAATAGAAAAGCTCTGTCTTGGTATCACCAGTTTAAAAGAATTACAGCGCGTC
CTGCATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

61.634

100

0.616
  pilB Vibrio parahaemolyticus RIMD 2210633

60.924

100

0.609
  pilB Vibrio campbellii strain DS40M4

60.391

100

0.604
  pilB Legionella pneumophila strain ERS1305867

50

96.27

0.481
  pilB Acinetobacter baylyi ADP1

48.905

97.336

0.476
  pilB Acinetobacter baumannii D1279779

50.958

92.718

0.472
  pilF Neisseria gonorrhoeae MS11

44.366

100

0.448
  pilB/pilB1 Synechocystis sp. PCC 6803

39.499

92.185

0.364
  pilB Haemophilus influenzae 86-028NP

41.856

86.146

0.361
  pilB Haemophilus influenzae Rd KW20

41.856

86.146

0.361