Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   N5938_RS10195 Genome accession   NZ_CP107257
Coordinates   2188560..2189024 (+) Length   154 a.a.
NCBI ID   WP_023129290.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2019CK-00034     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2183560..2194024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5938_RS10170 (N5938_10170) yacG 2183727..2183927 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  N5938_RS10175 (N5938_10175) coaE 2183924..2184535 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  N5938_RS10180 (N5938_10180) pilD 2184532..2185404 (-) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  N5938_RS10185 (N5938_10185) - 2185407..2186625 (-) 1219 Protein_2000 type II secretion system F family protein -
  N5938_RS10190 (N5938_10190) pilB 2186629..2188329 (-) 1701 WP_263901898.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N5938_RS10195 (N5938_10195) pilA/pilAI 2188560..2189024 (+) 465 WP_023129290.1 pilin Machinery gene
  N5938_RS10200 (N5938_10200) - 2189339..2189620 (+) 282 WP_263901899.1 hypothetical protein -
  N5938_RS10205 (N5938_10205) - 2189630..2190532 (+) 903 WP_263901900.1 O-antigen ligase family protein -
  N5938_RS10215 (N5938_10215) nadC 2190676..2191524 (-) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16148.47 Da        Isoelectric Point: 8.9986

>NTDB_id=642737 N5938_RS10195 WP_023129290.1 2188560..2189024(+) (pilA/pilAI) [Pseudomonas aeruginosa strain 2019CK-00034]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGDGKGQIKIDKADTATPEISGTLGRSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=642737 N5938_RS10195 WP_023129290.1 2188560..2189024(+) (pilA/pilAI) [Pseudomonas aeruginosa strain 2019CK-00034]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTGACGGTAAGGGGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCCGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.516

100

0.448
  pilA Acinetobacter baumannii strain A118

42.857

100

0.429
  pilA Pseudomonas aeruginosa PAK

41.139

100

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

37.059

100

0.409
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.073

98.052

0.383
  pilA Vibrio cholerae strain A1552

39.073

98.052

0.383
  pilA Vibrio cholerae C6706

39.073

98.052

0.383
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364