Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LZ605_RS10915 Genome accession   NZ_CP090423
Coordinates   2315200..2316294 (-) Length   364 a.a.
NCBI ID   WP_057497269.1    Uniprot ID   A0A0R0BAJ7
Organism   Stenotrophomonas maltophilia strain GYH     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2310200..2321294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZ605_RS10885 (LZ605_10885) - 2310432..2310977 (+) 546 WP_249844826.1 DNA-3-methyladenine glycosylase I -
  LZ605_RS10890 (LZ605_10890) - 2311012..2311719 (-) 708 WP_029379885.1 YitT family protein -
  LZ605_RS10895 (LZ605_10895) - 2311831..2312043 (-) 213 WP_249844827.1 DUF4287 domain-containing protein -
  LZ605_RS10900 (LZ605_10900) - 2312386..2313282 (+) 897 WP_249844828.1 LysR family transcriptional regulator -
  LZ605_RS10905 (LZ605_10905) - 2313341..2314324 (-) 984 WP_249844829.1 aldo/keto reductase -
  LZ605_RS10910 (LZ605_10910) - 2314436..2315113 (-) 678 WP_249844830.1 hypothetical protein -
  LZ605_RS10915 (LZ605_10915) pilU 2315200..2316294 (-) 1095 WP_057497269.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LZ605_RS10920 (LZ605_10920) pilT 2316442..2317479 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  LZ605_RS10925 (LZ605_10925) - 2317567..2318244 (+) 678 WP_249844831.1 YggS family pyridoxal phosphate-dependent enzyme -
  LZ605_RS10930 (LZ605_10930) proC 2318263..2319084 (+) 822 WP_249844832.1 pyrroline-5-carboxylate reductase -
  LZ605_RS10935 (LZ605_10935) - 2319091..2319966 (-) 876 WP_249844833.1 LysR family transcriptional regulator -
  LZ605_RS10940 (LZ605_10940) - 2320064..2320666 (+) 603 WP_249844834.1 short chain dehydrogenase -
  LZ605_RS10945 (LZ605_10945) soxR 2320709..2321143 (-) 435 WP_107230054.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40592.65 Da        Isoelectric Point: 6.8284

>NTDB_id=642285 LZ605_RS10915 WP_057497269.1 2315200..2316294(-) (pilU) [Stenotrophomonas maltophilia strain GYH]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNHNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIINFFPEDRR
NQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=642285 LZ605_RS10915 WP_057497269.1 2315200..2316294(-) (pilU) [Stenotrophomonas maltophilia strain GYH]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACCGCCGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCC
GATCACGCAGACGCCGCTGACGCCGCAGCAGAGCCGCGACCTGGTCCTCAATGTGATGACCCCGGCGCAGCGCGAGGAGT
TCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGTGTCAGCTGCTTCTACCAGCGC
AACCAGGTCGGCATGGTGCTGCGTCGCATCGAGACGCGCATCCCGACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCTACCGGTACCGGCAAGTCGACGTCGCTGGCGGCGA
TGATCGGCTATCGCAACCATAATTCGACCGGCCATATCATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAA
GGCTGCATCATCACCCAGCGCGAAGTGGGCATTGATACCGACAGCTGGGAAGCGGCGTTGAAGAACACCCTGCGCCAGGC
GCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCATGGACCATGCCATCGCCTTCGCCGAAACCGGCCATC
TGGTGCTGTGCACGCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGC
AACCAGCTGCTGATGGATCTTTCGCTGAACCTCAAGGGTGTGGTGGCGCAGCAGCTGGTGCCTTCGCCCGATGGTCGTTC
GCGCAAGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGATTACATCCGCGATGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGATTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAG
ATCAGCTACGAGGACGCATTGCGCTACGCCGATTCGCAGAACGAGGTACGCCTGCGCATCAAGCTCAGCCAGGGCGGCGA
CGCGCGCACCCTGTCGCAGGGGCTGGACGGCGTGGAGATTTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0R0BAJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.994

98.352

0.679
  pilU Acinetobacter baylyi ADP1

64.08

95.604

0.613
  pilU Vibrio cholerae strain A1552

54.179

95.33

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.609

94.78

0.404
  pilT Acinetobacter baumannii strain A118

40.606

90.659

0.368
  pilT Acinetobacter nosocomialis M2

40.606

90.659

0.368
  pilT Acinetobacter baumannii D1279779

40.606

90.659

0.368
  pilT Legionella pneumophila strain ERS1305867

40.867

88.736

0.363
  pilT Legionella pneumophila strain Lp02

40.867

88.736

0.363