Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LQE85_RS05175 Genome accession   NZ_CP088000
Coordinates   1088895..1090037 (+) Length   380 a.a.
NCBI ID   WP_068849744.1    Uniprot ID   -
Organism   Stenotrophomonas rhizophila strain Bl2-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1083895..1095037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQE85_RS05150 (LQE85_05150) - 1084212..1085570 (-) 1359 WP_249833853.1 MFS transporter -
  LQE85_RS05155 (LQE85_05155) soxR 1085660..1086094 (+) 435 WP_176472643.1 redox-sensitive transcriptional activator SoxR -
  LQE85_RS05160 (LQE85_05160) proC 1086101..1086919 (-) 819 WP_095363720.1 pyrroline-5-carboxylate reductase -
  LQE85_RS05165 (LQE85_05165) - 1086984..1087655 (-) 672 WP_249833854.1 YggS family pyridoxal phosphate-dependent enzyme -
  LQE85_RS05170 (LQE85_05170) pilT 1087771..1088808 (+) 1038 WP_068849741.1 type IV pilus twitching motility protein PilT Machinery gene
  LQE85_RS05175 (LQE85_05175) pilU 1088895..1090037 (+) 1143 WP_068849744.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LQE85_RS05180 (LQE85_05180) - 1090153..1090737 (+) 585 WP_249833855.1 hypothetical protein -
  LQE85_RS05185 (LQE85_05185) - 1090868..1091851 (+) 984 WP_249833856.1 aldo/keto reductase -
  LQE85_RS05190 (LQE85_05190) - 1091916..1092131 (+) 216 WP_068854565.1 DUF4287 domain-containing protein -
  LQE85_RS05195 (LQE85_05195) - 1092226..1092933 (+) 708 WP_184125063.1 YitT family protein -
  LQE85_RS05200 (LQE85_05200) - 1092938..1093465 (-) 528 WP_068849753.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42315.70 Da        Isoelectric Point: 6.7684

>NTDB_id=632231 LQE85_RS05175 WP_068849744.1 1088895..1090037(+) (pilU) [Stenotrophomonas rhizophila strain Bl2-2]
MNTTSTTIDFTSFLKLMAHQKASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNLNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNA
NQAMDRIINFFPEDRRSQLLMDLSLNLKGVVAQQLVPSPDGKSRKVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESVQLG
MRTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=632231 LQE85_RS05175 WP_068849744.1 1088895..1090037(+) (pilU) [Stenotrophomonas rhizophila strain Bl2-2]
ATGAATACCACCAGCACCACCATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTTTTCAT
CACCGCGGGCATGCCGCCTGCGATCAAGGTCAACGGCAAGATCTCGCCCATCACCCAGACCCCGCTCACGCCGCAGCAGA
GCCGCGACCTGGTGCTGAACGTGATGACGCCGGCGCAGCGCGAAGAGTTCGAAAAAACCCACGAGTGCAACTTCGCCATC
GGCCTGTCCGGCGTGGGCCGGTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTCCTGCGCCGCATTGA
AACCCGAATTCCCACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCC
TGTTCGTGGGTGCGACCGGTACCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGTTACCGCAACCTGAACTCCACCGGG
CACATCATCACCATCGAAGACCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATTATCACCCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCGGCGTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAAGTGCGTA
CCCGCGAGGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGTACGCTGCATGCCAACAACGCC
AACCAGGCGATGGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAGCCAGCTGCTGATGGATCTGTCGCTGAACCT
CAAGGGCGTGGTCGCCCAACAGCTGGTGCCCTCGCCGGATGGCAAGTCGCGCAAGGTGGCGATGGAAATCATGCTGGGCA
CGCCGCTGGTGCAGGATTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAATCGGTGCAGCTGGGC
ATGCGCACCTTCGACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAGATCAGCTACGAAGACGCGCTGCGCTACGCCGA
TTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCTCGCAGGGCGGTGACGCACGCACGCTGTCGCAGGGCCTGGACGGCG
TGGAGATTTCCGAAGTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

96.579

0.653
  pilU Acinetobacter baylyi ADP1

63.585

93.947

0.597
  pilU Vibrio cholerae strain A1552

53.371

93.684

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.179

94.211

0.397
  pilT Acinetobacter baumannii strain A118

40.407

90.526

0.366
  pilT Acinetobacter nosocomialis M2

40.407

90.526

0.366
  pilT Acinetobacter baumannii D1279779

40.407

90.526

0.366
  pilT Legionella pneumophila strain ERS1305867

41.194

88.158

0.363
  pilT Legionella pneumophila strain Lp02

41.194

88.158

0.363