Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LPH35_RS07700 Genome accession   NZ_CP087711
Coordinates   1688690..1689109 (-) Length   139 a.a.
NCBI ID   WP_230433054.1    Uniprot ID   -
Organism   Plesiomonas shigelloides strain 7A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1685026..1693182 1688690..1689109 within 0


Gene organization within MGE regions


Location: 1685026..1693182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPH35_RS07685 - 1685026..1685730 (-) 705 WP_230433051.1 pilin glycosylation ligase domain-containing protein -
  LPH35_RS07690 - 1685894..1687129 (-) 1236 WP_230433052.1 type II secretion system F family protein -
  LPH35_RS07695 pilB 1687131..1688639 (-) 1509 WP_230433053.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LPH35_RS07700 pilA 1688690..1689109 (-) 420 WP_230433054.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LPH35_RS07705 nadC 1689881..1690807 (-) 927 WP_230433055.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LPH35_RS07710 ampD 1691242..1691847 (+) 606 WP_230433056.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LPH35_RS07715 ampE 1692048..1692890 (+) 843 WP_116545976.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14556.75 Da        Isoelectric Point: 4.5066

>NTDB_id=631156 LPH35_RS07700 WP_230433054.1 1688690..1689109(-) (pilA) [Plesiomonas shigelloides strain 7A]
MKAVNKGFTLIELMIVVAIIGILAAIAIPAYQNYTKKAKFTEVVVATGPYKTAVELCTYDKGDVADCEEGETGSGYTITG
QASAIGLVNEIKVEANGVITATAVSNQGLEGETYILTPTLTGEKVTWQATGTCKDKGLC

Nucleotide


Download         Length: 420 bp        

>NTDB_id=631156 LPH35_RS07700 WP_230433054.1 1688690..1689109(-) (pilA) [Plesiomonas shigelloides strain 7A]
ATGAAAGCAGTGAACAAGGGTTTTACCCTGATTGAATTGATGATTGTGGTAGCGATTATTGGTATTTTGGCCGCAATTGC
CATACCAGCCTATCAGAACTACACCAAAAAAGCTAAGTTTACTGAGGTTGTAGTCGCTACAGGTCCATACAAAACTGCGG
TTGAATTGTGTACCTATGATAAAGGTGACGTTGCTGATTGTGAAGAAGGCGAAACGGGTTCTGGATATACAATTACAGGC
CAAGCTTCAGCAATTGGTTTAGTCAATGAAATTAAAGTTGAAGCCAACGGTGTAATTACTGCAACAGCAGTAAGTAACCA
AGGTTTGGAAGGCGAAACGTATATTCTAACACCAACACTTACCGGCGAAAAAGTAACATGGCAAGCAACTGGAACCTGTA
AAGACAAGGGACTTTGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-27)

Pilin (bacterial filament) (Pilin)

(37-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

48.905

98.561

0.482
  pilA Vibrio campbellii strain DS40M4

44.203

99.281

0.439
  pilA2 Legionella pneumophila strain ERS1305867

46.875

92.086

0.432
  pilA Haemophilus influenzae Rd KW20

44.444

97.122

0.432
  pilA2 Legionella pneumophila str. Paris

46.094

92.086

0.424
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.105

95.683

0.403
  pilA/pilA1 Eikenella corrodens VA1

36.735

100

0.388
  pilA Glaesserella parasuis strain SC1401

39.695

94.245

0.374
  comP Acinetobacter baylyi ADP1

37.778

97.122

0.367