Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   N4P55_RS04225 Genome accession   NZ_CP104408
Coordinates   978946..979359 (+) Length   137 a.a.
NCBI ID   WP_316963854.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain Ant01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 973946..984359
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4P55_RS04190 (N4P55_04200) - 974068..974697 (-) 630 WP_053254334.1 DUF1780 domain-containing protein -
  N4P55_RS04195 (N4P55_04205) - 974889..975578 (+) 690 WP_064450649.1 energy-coupling factor ABC transporter permease -
  N4P55_RS04200 (N4P55_04210) - 975643..975858 (+) 216 WP_064450650.1 hypothetical protein -
  N4P55_RS04205 (N4P55_04215) yacG 975855..976055 (-) 201 WP_064450651.1 DNA gyrase inhibitor YacG -
  N4P55_RS04210 (N4P55_04220) coaE 976052..976675 (-) 624 WP_064450652.1 dephospho-CoA kinase -
  N4P55_RS04215 (N4P55_04225) pilD 976672..977538 (-) 867 WP_316963683.1 A24 family peptidase Machinery gene
  N4P55_RS04220 (N4P55_04230) pilC 977535..978743 (-) 1209 WP_262667007.1 type II secretion system F family protein Machinery gene
  N4P55_RS04225 (N4P55_04235) pilA/pilAI 978946..979359 (+) 414 WP_316963854.1 pilin Machinery gene
  N4P55_RS04235 (N4P55_04245) - 979651..979956 (+) 306 WP_221293915.1 DUF6388 family protein -
  N4P55_RS04240 (N4P55_04250) nadC 980017..980865 (-) 849 WP_064450657.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N4P55_RS04245 (N4P55_04255) - 981110..983284 (+) 2175 WP_262667009.1 DUF1631 domain-containing protein -
  N4P55_RS04250 (N4P55_04260) ampD 983328..983891 (+) 564 WP_064450659.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 13909.16 Da        Isoelectric Point: 7.6356

>NTDB_id=629296 N4P55_RS04225 WP_316963854.1 978946..979359(+) (pilA/pilAI) [Pseudomonas fluorescens strain Ant01]
MRQKGFTLIELLIVVAIIGILATIGLPMYTKHQAKAKFTAGLAEVSALKAGFEDTLNQGTVPTLALIGGTSPTANCTIEV
AGDVATGTGSIRCEILDAPAPVLGKTITLTRSATTGWKCATTADAQYVAKGCGADGA

Nucleotide


Download         Length: 414 bp        

>NTDB_id=629296 N4P55_RS04225 WP_316963854.1 978946..979359(+) (pilA/pilAI) [Pseudomonas fluorescens strain Ant01]
ATGCGTCAGAAAGGCTTCACCTTGATCGAGTTGTTGATCGTCGTGGCAATCATCGGCATTTTGGCCACGATCGGTTTGCC
CATGTATACCAAGCACCAGGCCAAGGCCAAGTTTACGGCGGGGTTGGCTGAAGTCTCCGCGTTGAAGGCCGGTTTCGAAG
ACACCCTTAACCAGGGCACCGTGCCGACACTGGCCTTGATCGGTGGCACCAGCCCAACCGCCAACTGCACGATCGAGGTG
GCAGGCGATGTGGCCACCGGCACCGGTTCCATCCGTTGTGAAATTCTCGATGCGCCCGCTCCCGTGCTGGGCAAGACCAT
CACGTTGACGCGCAGTGCCACCACCGGCTGGAAGTGTGCAACCACGGCTGACGCTCAATACGTTGCCAAGGGCTGCGGCG
CCGACGGAGCATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)

Pilin (bacterial filament) (Pilin)

(34-132)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.588

99.27

0.453
  pilA Pseudomonas aeruginosa PAK

41.497

100

0.445
  comP Acinetobacter baylyi ADP1

38.776

100

0.416
  pilA Acinetobacter baumannii strain A118

41.912

99.27

0.416
  pilA Vibrio cholerae C6706

39.583

100

0.416
  pilA Vibrio cholerae strain A1552

39.583

100

0.416
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.583

100

0.416
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40

98.54

0.394
  pilA Vibrio parahaemolyticus RIMD 2210633

37.594

97.08

0.365