Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY53_RS25085 Genome accession   NZ_CP087181
Coordinates   5631988..5633256 (-) Length   422 a.a.
NCBI ID   WP_252826126.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-031     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5626988..5638256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY53_RS25070 (LOY53_25055) - 5628630..5630255 (-) 1626 WP_252826125.1 AAA family ATPase -
  LOY53_RS25075 (LOY53_25060) aroB 5630271..5631368 (-) 1098 WP_099455610.1 3-dehydroquinate synthase -
  LOY53_RS25080 (LOY53_25065) aroK 5631465..5631983 (-) 519 WP_027592357.1 shikimate kinase AroK -
  LOY53_RS25085 (LOY53_25070) pilQ 5631988..5633256 (-) 1269 WP_252826126.1 type IV pilus secretin PilQ Machinery gene
  LOY53_RS25090 (LOY53_25075) - 5633180..5634163 (-) 984 WP_252826127.1 pilus assembly protein PilP -
  LOY53_RS25095 (LOY53_25080) - 5634193..5634729 (-) 537 WP_252826128.1 PilN domain-containing protein -
  LOY53_RS25100 (LOY53_25085) pilM 5634726..5635589 (-) 864 WP_252826129.1 type IV pilus biogenesis protein PilM -
  LOY53_RS25105 (LOY53_25090) - 5635771..5638221 (+) 2451 WP_371918104.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45670.40 Da        Isoelectric Point: 5.2046

>NTDB_id=627486 LOY53_RS25085 WP_252826126.1 5631988..5633256(-) (pilQ) [Pseudomonas sp. B21-031]
MKRLWIGAAAFVLSIPLATAVPTRGEPLSLNFQDVQVRAVLQVLADHAGVNLVASDSVQGNITLRLQDVPWEQALDLVLR
SKGLARREEGNVLLVAPAAELAGQSLDARLGQALDAQLQPLQRELMPIHHAVASELAELLLATLADDGILTGRGSLSVDA
RTNTLVAYQPADRLAELRHLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGENVRPGKELFVDLGVERAGSSV
GLGLLRGGILLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVSFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDVLQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=627486 LOY53_RS25085 WP_252826126.1 5631988..5633256(-) (pilQ) [Pseudomonas sp. B21-031]
ATGAAAAGGCTATGGATAGGGGCGGCGGCGTTCGTCTTGTCGATCCCGCTAGCGACGGCAGTGCCCACTCGGGGTGAGCC
ACTGTCGCTGAACTTTCAGGATGTGCAGGTACGCGCCGTATTGCAGGTGCTGGCAGACCATGCCGGCGTGAACCTGGTCG
CCAGCGATTCGGTGCAGGGCAATATCACCTTGCGGCTTCAGGATGTGCCCTGGGAGCAGGCGCTGGACCTGGTACTGCGC
AGCAAGGGCCTGGCCCGCCGCGAGGAGGGCAACGTGCTGTTGGTGGCGCCGGCGGCGGAGCTTGCTGGGCAATCGCTCGA
TGCCCGACTAGGCCAGGCACTGGATGCGCAATTGCAACCGCTGCAGCGTGAACTGATGCCGATCCACCATGCCGTAGCCT
CCGAGCTCGCGGAGCTGTTGCTGGCAACCCTGGCGGACGACGGCATCCTCACCGGGCGCGGCAGTTTGAGCGTTGATGCG
CGCACCAATACCCTGGTGGCCTACCAGCCGGCTGACCGCCTTGCCGAGCTGCGCCACCTCGTGGCGCAGCTGGATGTGCC
GGTGCGTCAGGTGGCAATTGAGGCACGTATCGTCGAGGCCAATGTCGACTACGAAAAGAGCCTTGGGGTGCGTTGGGGTG
GGCCCTTGTACGGTGAGAACGTAAGGCCAGGCAAGGAGCTGTTCGTCGACCTTGGGGTGGAACGTGCCGGCTCCAGTGTT
GGCCTGGGCCTGCTGCGTGGCGGCATCTTGCTAGACCTTGAGCTCAGCGCCATGGAAAAGAGCGGCAATGGCGAAATTAT
CTCGCAGCCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTCAAGGGTACCGAGGTGCCTTACCAGGAAACCA
GCAAGAGCGGCGCCACGTCCGTGTCGTTTCGCGAAGCGTCCCTGTCGCTTGAAGTCACCCCGCAGATTACCCCGGACAAC
AAGGTCATCATGACGGTCAGGGTGACCAAGGACGAACCTGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAA
GAACGAGGTCAATGCCAAGGTTCGCGTGGCGGACGGCGAGACGATCGTGATCGGTGGCGTCTACTCGACCACGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGTATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.677

100

0.614
  comQ Acinetobacter baylyi ADP1

42.857

100

0.455
  pilQ Acinetobacter baumannii strain A118

42.105

100

0.455
  pilQ Acinetobacter baumannii D1279779

42.105

100

0.455
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.518

99.763

0.424
  pilQ Vibrio cholerae strain A1552

42.518

99.763

0.424
  pilQ Legionella pneumophila strain ERS1305867

42.788

98.578

0.422
  pilQ Legionella pneumophila strain Lp02

42.788

98.578

0.422
  pilQ Vibrio campbellii strain DS40M4

40.278

100

0.412
  pilQ Neisseria meningitidis MC58

36.364

99.052

0.36